Detection of infectious tobamoviruses in irrigation and drainage canals in Greater Poland
Water samples were collected from irrigation ditches and drainage canals surrounding fields in southern Greater Poland. Initially, the samples were subjected to low and highspeed centrifugation and obtained pellets were used to perform biological assays. Viral identification involved biological,...
Main Authors: | , , |
---|---|
Format: | Article |
Language: | English |
Published: |
Polish Academy of Sciences
2018-06-01
|
Series: | Journal of Plant Protection Research |
Subjects: | |
Online Access: | https://doi.org/10.24425/119126 |
_version_ | 1798018329240141824 |
---|---|
author | Małgorzata Jeżewska Katarzyna Trzmiel Aleksandra Zarzyńska-Nowak |
author_facet | Małgorzata Jeżewska Katarzyna Trzmiel Aleksandra Zarzyńska-Nowak |
author_sort | Małgorzata Jeżewska |
collection | DOAJ |
description | Water samples were collected from irrigation ditches and drainage canals surrounding
fields in southern Greater Poland. Initially, the samples were subjected to low and highspeed
centrifugation and obtained pellets were used to perform biological assays. Viral
identification involved biological, electron microscopic as well as molecular methods. The
occurrence of Tobacco mosaic virus (TMV) and Tomato mosaic virus (ToMV) was demonstrated
in 12 of the 17 examined water sources. The molecular analysis results showed TMV
and ToMV co-infections in the analysed water samples. To our knowledge, this is the first
report of tobamoviruses being found in environmental water in Poland. |
first_indexed | 2024-04-11T16:22:18Z |
format | Article |
id | doaj.art-81cdbacbafb646f3bb69bf58a16bc39e |
institution | Directory Open Access Journal |
issn | 1899-007X 1899-007X |
language | English |
last_indexed | 2024-04-11T16:22:18Z |
publishDate | 2018-06-01 |
publisher | Polish Academy of Sciences |
record_format | Article |
series | Journal of Plant Protection Research |
spelling | doaj.art-81cdbacbafb646f3bb69bf58a16bc39e2022-12-22T04:14:18ZengPolish Academy of SciencesJournal of Plant Protection Research1899-007X1899-007X2018-06-0158220220510.24425/119126Detection of infectious tobamoviruses in irrigation and drainage canals in Greater PolandMałgorzata Jeżewska0Katarzyna Trzmiel1Aleksandra Zarzyńska-Nowak2Virology and Bacteriology, Institute of Plant Protection – National Research Institute, Władysława Węgorka 20, 60-318 Poznań, PolandVirology and Bacteriology, Institute of Plant Protection – National Research Institute, Władysława Węgorka 20, 60-318 Poznań, PolandVirology and Bacteriology, Institute of Plant Protection – National Research Institute, Władysława Węgorka 20, 60-318 Poznań, PolandWater samples were collected from irrigation ditches and drainage canals surrounding fields in southern Greater Poland. Initially, the samples were subjected to low and highspeed centrifugation and obtained pellets were used to perform biological assays. Viral identification involved biological, electron microscopic as well as molecular methods. The occurrence of Tobacco mosaic virus (TMV) and Tomato mosaic virus (ToMV) was demonstrated in 12 of the 17 examined water sources. The molecular analysis results showed TMV and ToMV co-infections in the analysed water samples. To our knowledge, this is the first report of tobamoviruses being found in environmental water in Poland.https://doi.org/10.24425/119126RT-PCRtobamoviruseswater-borne viruses |
spellingShingle | Małgorzata Jeżewska Katarzyna Trzmiel Aleksandra Zarzyńska-Nowak Detection of infectious tobamoviruses in irrigation and drainage canals in Greater Poland Journal of Plant Protection Research RT-PCR tobamoviruses water-borne viruses |
title | Detection of infectious tobamoviruses in irrigation and drainage canals in Greater Poland |
title_full | Detection of infectious tobamoviruses in irrigation and drainage canals in Greater Poland |
title_fullStr | Detection of infectious tobamoviruses in irrigation and drainage canals in Greater Poland |
title_full_unstemmed | Detection of infectious tobamoviruses in irrigation and drainage canals in Greater Poland |
title_short | Detection of infectious tobamoviruses in irrigation and drainage canals in Greater Poland |
title_sort | detection of infectious tobamoviruses in irrigation and drainage canals in greater poland |
topic | RT-PCR tobamoviruses water-borne viruses |
url | https://doi.org/10.24425/119126 |
work_keys_str_mv | AT małgorzatajezewska detectionofinfectioustobamovirusesinirrigationanddrainagecanalsingreaterpoland AT katarzynatrzmiel detectionofinfectioustobamovirusesinirrigationanddrainagecanalsingreaterpoland AT aleksandrazarzynskanowak detectionofinfectioustobamovirusesinirrigationanddrainagecanalsingreaterpoland |