Detection of infectious tobamoviruses in irrigation and drainage canals in Greater Poland

Water samples were collected from irrigation ditches and drainage canals surrounding fields in southern Greater Poland. Initially, the samples were subjected to low and highspeed centrifugation and obtained pellets were used to perform biological assays. Viral identification involved biological,...

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Main Authors: Małgorzata Jeżewska, Katarzyna Trzmiel, Aleksandra Zarzyńska-Nowak
Format: Article
Language:English
Published: Polish Academy of Sciences 2018-06-01
Series:Journal of Plant Protection Research
Subjects:
Online Access:https://doi.org/10.24425/119126
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author Małgorzata Jeżewska
Katarzyna Trzmiel
Aleksandra Zarzyńska-Nowak
author_facet Małgorzata Jeżewska
Katarzyna Trzmiel
Aleksandra Zarzyńska-Nowak
author_sort Małgorzata Jeżewska
collection DOAJ
description Water samples were collected from irrigation ditches and drainage canals surrounding fields in southern Greater Poland. Initially, the samples were subjected to low and highspeed centrifugation and obtained pellets were used to perform biological assays. Viral identification involved biological, electron microscopic as well as molecular methods. The occurrence of Tobacco mosaic virus (TMV) and Tomato mosaic virus (ToMV) was demonstrated in 12 of the 17 examined water sources. The molecular analysis results showed TMV and ToMV co-infections in the analysed water samples. To our knowledge, this is the first report of tobamoviruses being found in environmental water in Poland.
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spelling doaj.art-81cdbacbafb646f3bb69bf58a16bc39e2022-12-22T04:14:18ZengPolish Academy of SciencesJournal of Plant Protection Research1899-007X1899-007X2018-06-0158220220510.24425/119126Detection of infectious tobamoviruses in irrigation and drainage canals in Greater PolandMałgorzata Jeżewska0Katarzyna Trzmiel1Aleksandra Zarzyńska-Nowak2Virology and Bacteriology, Institute of Plant Protection – National Research Institute, Władysława Węgorka 20, 60-318 Poznań, PolandVirology and Bacteriology, Institute of Plant Protection – National Research Institute, Władysława Węgorka 20, 60-318 Poznań, PolandVirology and Bacteriology, Institute of Plant Protection – National Research Institute, Władysława Węgorka 20, 60-318 Poznań, PolandWater samples were collected from irrigation ditches and drainage canals surrounding fields in southern Greater Poland. Initially, the samples were subjected to low and highspeed centrifugation and obtained pellets were used to perform biological assays. Viral identification involved biological, electron microscopic as well as molecular methods. The occurrence of Tobacco mosaic virus (TMV) and Tomato mosaic virus (ToMV) was demonstrated in 12 of the 17 examined water sources. The molecular analysis results showed TMV and ToMV co-infections in the analysed water samples. To our knowledge, this is the first report of tobamoviruses being found in environmental water in Poland.https://doi.org/10.24425/119126RT-PCRtobamoviruseswater-borne viruses
spellingShingle Małgorzata Jeżewska
Katarzyna Trzmiel
Aleksandra Zarzyńska-Nowak
Detection of infectious tobamoviruses in irrigation and drainage canals in Greater Poland
Journal of Plant Protection Research
RT-PCR
tobamoviruses
water-borne viruses
title Detection of infectious tobamoviruses in irrigation and drainage canals in Greater Poland
title_full Detection of infectious tobamoviruses in irrigation and drainage canals in Greater Poland
title_fullStr Detection of infectious tobamoviruses in irrigation and drainage canals in Greater Poland
title_full_unstemmed Detection of infectious tobamoviruses in irrigation and drainage canals in Greater Poland
title_short Detection of infectious tobamoviruses in irrigation and drainage canals in Greater Poland
title_sort detection of infectious tobamoviruses in irrigation and drainage canals in greater poland
topic RT-PCR
tobamoviruses
water-borne viruses
url https://doi.org/10.24425/119126
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AT katarzynatrzmiel detectionofinfectioustobamovirusesinirrigationanddrainagecanalsingreaterpoland
AT aleksandrazarzynskanowak detectionofinfectioustobamovirusesinirrigationanddrainagecanalsingreaterpoland