Observation of the influences of diosgenin on aging ovarian reserve and function in a mouse model
Abstract Background The aim of this study was to investigate the impact of diosgenin, an important monomer of sapogenins in yams, on ovarian reserve in a natural aging mice model. Study design This randomized controlled trial included 60 9-month-old C57 naturally aging female mice. Twenty-one mice w...
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| Format: | Article |
| Language: | English |
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BMC
2017-10-01
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| Series: | European Journal of Medical Research |
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| Online Access: | http://link.springer.com/article/10.1186/s40001-017-0285-6 |
| _version_ | 1818285123587538944 |
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| author | Mingjie Shen Cong Qi Yan-Ping Kuang Yang Yang Qi-Feng Lyu Hui Long Zhi-Guang Yan Ying-Yu Lu |
| author_facet | Mingjie Shen Cong Qi Yan-Ping Kuang Yang Yang Qi-Feng Lyu Hui Long Zhi-Guang Yan Ying-Yu Lu |
| author_sort | Mingjie Shen |
| collection | DOAJ |
| description | Abstract Background The aim of this study was to investigate the impact of diosgenin, an important monomer of sapogenins in yams, on ovarian reserve in a natural aging mice model. Study design This randomized controlled trial included 60 9-month-old C57 naturally aging female mice. Twenty-one mice were assigned to the dio group and were fed a single dose of diosgenin (200 mg/kg/day) suspended in 0.3% CMC. Twenty mice were assigned to the DHEA group and were fed a single dose of DHEA (1.25 mg/kg/day) suspended in 0.3% CMC. The remaining 20 mice were assigned to the old control group and were fed a single dose of 0.3% CMC. Three months later, the reproductive performance of these female mice was determined by evaluating ovarian follicles and oocyte number and quality in IVF and comparing age-matched and young controls. The impact of NOBOX, GDF9 and BMP15 mRNA expression was also evaluated. Results Diosgenin improves ovarian reserve in naturally aging mice in terms of increasing the number of primary follicles (P < 0.05) and serum levels of AMH (P < 0.05). Conclusions Diosgenin could counteract age-associated ovarian dysfunction by improving the ovarian reserve in a natural aging mice model. |
| first_indexed | 2024-12-13T01:03:42Z |
| format | Article |
| id | doaj.art-81cf25c50c4545b883ce3bef776d711c |
| institution | Directory Open Access Journal |
| issn | 2047-783X |
| language | English |
| last_indexed | 2024-12-13T01:03:42Z |
| publishDate | 2017-10-01 |
| publisher | BMC |
| record_format | Article |
| series | European Journal of Medical Research |
| spelling | doaj.art-81cf25c50c4545b883ce3bef776d711c2022-12-22T00:04:36ZengBMCEuropean Journal of Medical Research2047-783X2017-10-012211710.1186/s40001-017-0285-6Observation of the influences of diosgenin on aging ovarian reserve and function in a mouse modelMingjie Shen0Cong Qi1Yan-Ping Kuang2Yang Yang3Qi-Feng Lyu4Hui Long5Zhi-Guang Yan6Ying-Yu Lu7Department of Gynecology and Obstetrics, Shu Guang Hospital Affiliated to Shanghai University of Traditional Chinese MedicineDepartment of Gynecology and Obstetrics, Shu Guang Hospital Affiliated to Shanghai University of Traditional Chinese MedicineDepartment of Assisted Reproduction, Shanghai Ninth People’s Hospital Affiliated to Shanghai Jiao Tong University of MedicineLaboratory of Immunology and Virology, Experiment Center for Science and Technology, Shanghai University of Traditional Chinese MedicineDepartment of Assisted Reproduction, Shanghai Ninth People’s Hospital Affiliated to Shanghai Jiao Tong University of MedicineDepartment of Assisted Reproduction, Shanghai Ninth People’s Hospital Affiliated to Shanghai Jiao Tong University of MedicineDepartment of Assisted Reproduction, Shanghai Ninth People’s Hospital Affiliated to Shanghai Jiao Tong University of MedicineDepartment of Gynecology and Obstetrics, Shu Guang Hospital Affiliated to Shanghai University of Traditional Chinese MedicineAbstract Background The aim of this study was to investigate the impact of diosgenin, an important monomer of sapogenins in yams, on ovarian reserve in a natural aging mice model. Study design This randomized controlled trial included 60 9-month-old C57 naturally aging female mice. Twenty-one mice were assigned to the dio group and were fed a single dose of diosgenin (200 mg/kg/day) suspended in 0.3% CMC. Twenty mice were assigned to the DHEA group and were fed a single dose of DHEA (1.25 mg/kg/day) suspended in 0.3% CMC. The remaining 20 mice were assigned to the old control group and were fed a single dose of 0.3% CMC. Three months later, the reproductive performance of these female mice was determined by evaluating ovarian follicles and oocyte number and quality in IVF and comparing age-matched and young controls. The impact of NOBOX, GDF9 and BMP15 mRNA expression was also evaluated. Results Diosgenin improves ovarian reserve in naturally aging mice in terms of increasing the number of primary follicles (P < 0.05) and serum levels of AMH (P < 0.05). Conclusions Diosgenin could counteract age-associated ovarian dysfunction by improving the ovarian reserve in a natural aging mice model.http://link.springer.com/article/10.1186/s40001-017-0285-6DiosgeninOvarian reserveAgingMouse model |
| spellingShingle | Mingjie Shen Cong Qi Yan-Ping Kuang Yang Yang Qi-Feng Lyu Hui Long Zhi-Guang Yan Ying-Yu Lu Observation of the influences of diosgenin on aging ovarian reserve and function in a mouse model European Journal of Medical Research Diosgenin Ovarian reserve Aging Mouse model |
| title | Observation of the influences of diosgenin on aging ovarian reserve and function in a mouse model |
| title_full | Observation of the influences of diosgenin on aging ovarian reserve and function in a mouse model |
| title_fullStr | Observation of the influences of diosgenin on aging ovarian reserve and function in a mouse model |
| title_full_unstemmed | Observation of the influences of diosgenin on aging ovarian reserve and function in a mouse model |
| title_short | Observation of the influences of diosgenin on aging ovarian reserve and function in a mouse model |
| title_sort | observation of the influences of diosgenin on aging ovarian reserve and function in a mouse model |
| topic | Diosgenin Ovarian reserve Aging Mouse model |
| url | http://link.springer.com/article/10.1186/s40001-017-0285-6 |
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