The Effects of Apelin on Mesenteric Ischemia and Reperfusion Damage in an Experimental Rat Model
Objective: Intestinal ischemia-reperfusion (I/R) injury is associated with high morbidity and mortality rates. There is ongoing research to find an effective preventive or treatment agent. We aimed to evaluate the effects of apelin 13 (AP) on intestinal I/R injury in a rat model. Material and Method...
| Main Authors: | , , , , , , |
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| Format: | Article |
| Language: | English |
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Galenos Publishing House
2012-06-01
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| Series: | Balkan Medical Journal |
| Subjects: | |
| Online Access: | http://balkanmedicaljournal.org/text.php3?id=852 |
| _version_ | 1797915800181407744 |
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| author | Mustafa Burak Sayhan Tulin Yalta Elif Çopuroğlu Gönül Sağıroğlu Serhat Oğuz Tamer Sağıroğlu Mehmet Ali Yağcı |
| author_facet | Mustafa Burak Sayhan Tulin Yalta Elif Çopuroğlu Gönül Sağıroğlu Serhat Oğuz Tamer Sağıroğlu Mehmet Ali Yağcı |
| author_sort | Mustafa Burak Sayhan |
| collection | DOAJ |
| description | Objective: Intestinal ischemia-reperfusion (I/R) injury is associated with high morbidity and mortality rates. There is ongoing research to find an effective preventive or treatment agent. We aimed to evaluate the effects of apelin 13 (AP) on intestinal I/R injury in a rat model. Material and Methods: Twenty-four male Sprague-Dawley rats aged 6-8 weeks and weighing 280±20 g were equally divided into three groups (control, I/R and I/R+AP). The control group underwent superior mesenteric artery (SMA) mobilization alone without any clamping. In the I/R and I/R+AP groups, an atraumatic microvascular bulldog clamp was placed across the SMA at its point of origin from the aorta. In the I/R+AP group, 2 µg/kg/d apelin was administered intraperitoneally. After 60 minutes of ischemia, relaparotomy was performed to remove the microvascular clamp on the SMA for 3 hours of reperfusion. After 3 hours, tissue samples were obtained for biochemical [malondialdehyde (MDA) and glutathione (GSH) levels] and histopathological analyses.Results: MDA levels were significantly higher in the I/R group compared to the control group. Although MDA levels were lower in the I/R+AP group compared tothe I/R group, the difference was not statistically significant. There was also no significant difference between the I/R+AP and I/R groups regarding GSH levels. The median histopathological grade was significantly lower in the I/R+AP group compared to the I/R group (p=0.001).Conclusion: Apelin appeared to have a positive effect on oxidative injury; this did not reach statistical significance. Thus, the role of apelin and associated findings in the initial treatment of intestinal ischemia needs further large-scale animal studies before human use. |
| first_indexed | 2024-04-10T12:47:31Z |
| format | Article |
| id | doaj.art-81d40811b6564b20b68a7cb3c1c2f815 |
| institution | Directory Open Access Journal |
| issn | 2146-3123 2146-3131 |
| language | English |
| last_indexed | 2024-04-10T12:47:31Z |
| publishDate | 2012-06-01 |
| publisher | Galenos Publishing House |
| record_format | Article |
| series | Balkan Medical Journal |
| spelling | doaj.art-81d40811b6564b20b68a7cb3c1c2f8152023-02-15T16:14:00ZengGalenos Publishing HouseBalkan Medical Journal2146-31232146-31312012-06-01292148152The Effects of Apelin on Mesenteric Ischemia and Reperfusion Damage in an Experimental Rat ModelMustafa Burak SayhanTulin YaltaElif ÇopuroğluGönül SağıroğluSerhat OğuzTamer SağıroğluMehmet Ali YağcıObjective: Intestinal ischemia-reperfusion (I/R) injury is associated with high morbidity and mortality rates. There is ongoing research to find an effective preventive or treatment agent. We aimed to evaluate the effects of apelin 13 (AP) on intestinal I/R injury in a rat model. Material and Methods: Twenty-four male Sprague-Dawley rats aged 6-8 weeks and weighing 280±20 g were equally divided into three groups (control, I/R and I/R+AP). The control group underwent superior mesenteric artery (SMA) mobilization alone without any clamping. In the I/R and I/R+AP groups, an atraumatic microvascular bulldog clamp was placed across the SMA at its point of origin from the aorta. In the I/R+AP group, 2 µg/kg/d apelin was administered intraperitoneally. After 60 minutes of ischemia, relaparotomy was performed to remove the microvascular clamp on the SMA for 3 hours of reperfusion. After 3 hours, tissue samples were obtained for biochemical [malondialdehyde (MDA) and glutathione (GSH) levels] and histopathological analyses.Results: MDA levels were significantly higher in the I/R group compared to the control group. Although MDA levels were lower in the I/R+AP group compared tothe I/R group, the difference was not statistically significant. There was also no significant difference between the I/R+AP and I/R groups regarding GSH levels. The median histopathological grade was significantly lower in the I/R+AP group compared to the I/R group (p=0.001).Conclusion: Apelin appeared to have a positive effect on oxidative injury; this did not reach statistical significance. Thus, the role of apelin and associated findings in the initial treatment of intestinal ischemia needs further large-scale animal studies before human use.http://balkanmedicaljournal.org/text.php3?id=852Apelinmesenteric ischemiareperfusion injury |
| spellingShingle | Mustafa Burak Sayhan Tulin Yalta Elif Çopuroğlu Gönül Sağıroğlu Serhat Oğuz Tamer Sağıroğlu Mehmet Ali Yağcı The Effects of Apelin on Mesenteric Ischemia and Reperfusion Damage in an Experimental Rat Model Balkan Medical Journal Apelin mesenteric ischemia reperfusion injury |
| title | The Effects of Apelin on Mesenteric Ischemia and Reperfusion Damage in an Experimental Rat Model |
| title_full | The Effects of Apelin on Mesenteric Ischemia and Reperfusion Damage in an Experimental Rat Model |
| title_fullStr | The Effects of Apelin on Mesenteric Ischemia and Reperfusion Damage in an Experimental Rat Model |
| title_full_unstemmed | The Effects of Apelin on Mesenteric Ischemia and Reperfusion Damage in an Experimental Rat Model |
| title_short | The Effects of Apelin on Mesenteric Ischemia and Reperfusion Damage in an Experimental Rat Model |
| title_sort | effects of apelin on mesenteric ischemia and reperfusion damage in an experimental rat model |
| topic | Apelin mesenteric ischemia reperfusion injury |
| url | http://balkanmedicaljournal.org/text.php3?id=852 |
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