Gut microbiota composition and changes in patients with sepsis: potential markers for predicting survival
Abstract Background Sepsis can cause immune dysregulation and multiple organ failure in patients and eventually lead to death. The gut microbiota has demonstrated its precise therapeutic potential in the treatment of various diseases. This study aimed to discuss the structural changes of the gut mic...
| Main Authors: | , , , , , , , , , , , , , , |
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| Format: | Article |
| Language: | English |
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BMC
2024-02-01
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| Series: | BMC Microbiology |
| Subjects: | |
| Online Access: | https://doi.org/10.1186/s12866-024-03188-6 |
| _version_ | 1797275623849197568 |
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| author | Feiyu Luan Yang Zhou Xiaohui Ma Yue Li Yahui Peng Xiaonan Jia Nana Li Xibo Wang Yinghao Luo Mingyin Man Qianqian Zhang Chunying Wang Kaijiang Yu Mingyan Zhao Changsong Wang |
| author_facet | Feiyu Luan Yang Zhou Xiaohui Ma Yue Li Yahui Peng Xiaonan Jia Nana Li Xibo Wang Yinghao Luo Mingyin Man Qianqian Zhang Chunying Wang Kaijiang Yu Mingyan Zhao Changsong Wang |
| author_sort | Feiyu Luan |
| collection | DOAJ |
| description | Abstract Background Sepsis can cause immune dysregulation and multiple organ failure in patients and eventually lead to death. The gut microbiota has demonstrated its precise therapeutic potential in the treatment of various diseases. This study aimed to discuss the structural changes of the gut microbiota in patients with sepsis and to analyze the differences in the gut microbiota of patients with different prognoses. Methods We conducted a multicenter study in which rectal swab specimens were collected on the first and third days of sepsis diagnosis. A total of 70 specimens were collected, and gut microbiota information was obtained by 16S rRNA analysis. Results The relative abundance of Enterococcus decreased in rectal swab specimens during the first three days of diagnosis in patients with sepsis, while the relative abundance of inflammation-associated Bacillus species such as Escherichia coli, Enterobacteriaceae, and Bacteroidetes increased. By comparing the differences in the flora of the survival group and the death group, we found that the abundance of Veillonella and Ruminococcus in the death group showed an increasing trend (p < 0.05), while the abundance of Prevotella_6 and Prevotella_sp_S4_BM14 was increased in surviving patients (p < 0.05). Conclusions The Firmicutes/Bacteroidetes ratio, reflecting overall gut microbial composition, was significantly lower on day three of sepsis diagnosis. Changes in the abundance of specific gut microbiota may serve as prognostic markers in patients with sepsis. |
| first_indexed | 2024-03-07T15:17:07Z |
| format | Article |
| id | doaj.art-81d42b2bf2a945af886e340d838a3b9e |
| institution | Directory Open Access Journal |
| issn | 1471-2180 |
| language | English |
| last_indexed | 2024-03-07T15:17:07Z |
| publishDate | 2024-02-01 |
| publisher | BMC |
| record_format | Article |
| series | BMC Microbiology |
| spelling | doaj.art-81d42b2bf2a945af886e340d838a3b9e2024-03-05T17:51:47ZengBMCBMC Microbiology1471-21802024-02-0124111110.1186/s12866-024-03188-6Gut microbiota composition and changes in patients with sepsis: potential markers for predicting survivalFeiyu Luan0Yang Zhou1Xiaohui Ma2Yue Li3Yahui Peng4Xiaonan Jia5Nana Li6Xibo Wang7Yinghao Luo8Mingyin Man9Qianqian Zhang10Chunying Wang11Kaijiang Yu12Mingyan Zhao13Changsong Wang14Departments of Critical Care Medicine, the First Affiliated Hospital of Harbin Medical University, Harbin Medical UniversityDepartments of Critical Care Medicine, the First Affiliated Hospital of Harbin Medical University, Harbin Medical UniversityDepartments of Critical Care Medicine, the First Affiliated Hospital of Harbin Medical University, Harbin Medical UniversityDepartments of Critical Care Medicine, the Second Affiliated Hospital of Harbin Medical University, Harbin Medical UniversityDepartments of Critical Care Medicine, the First Affiliated Hospital of Harbin Medical University, Harbin Medical UniversityDepartments of Critical Care Medicine, the First Affiliated Hospital of Harbin Medical University, Harbin Medical UniversityDepartments of Critical Care Medicine, the First Affiliated Hospital of Harbin Medical University, Harbin Medical UniversityDepartments of Critical Care Medicine, the First Affiliated Hospital of Harbin Medical University, Harbin Medical UniversityDepartments of Critical Care Medicine, the First Affiliated Hospital of Harbin Medical University, Harbin Medical UniversityDepartments of Critical Care Medicine, the First Affiliated Hospital of Harbin Medical University, Harbin Medical UniversityDepartments of Critical Care Medicine, the First Affiliated Hospital of Harbin Medical University, Harbin Medical UniversityDepartments of Critical Care Medicine, the First Affiliated Hospital of Harbin Medical University, Harbin Medical UniversityDepartments of Critical Care Medicine, the First Affiliated Hospital of Harbin Medical University, Harbin Medical UniversityDepartments of Critical Care Medicine, the First Affiliated Hospital of Harbin Medical University, Harbin Medical UniversityDepartments of Critical Care Medicine, the First Affiliated Hospital of Harbin Medical University, Harbin Medical UniversityAbstract Background Sepsis can cause immune dysregulation and multiple organ failure in patients and eventually lead to death. The gut microbiota has demonstrated its precise therapeutic potential in the treatment of various diseases. This study aimed to discuss the structural changes of the gut microbiota in patients with sepsis and to analyze the differences in the gut microbiota of patients with different prognoses. Methods We conducted a multicenter study in which rectal swab specimens were collected on the first and third days of sepsis diagnosis. A total of 70 specimens were collected, and gut microbiota information was obtained by 16S rRNA analysis. Results The relative abundance of Enterococcus decreased in rectal swab specimens during the first three days of diagnosis in patients with sepsis, while the relative abundance of inflammation-associated Bacillus species such as Escherichia coli, Enterobacteriaceae, and Bacteroidetes increased. By comparing the differences in the flora of the survival group and the death group, we found that the abundance of Veillonella and Ruminococcus in the death group showed an increasing trend (p < 0.05), while the abundance of Prevotella_6 and Prevotella_sp_S4_BM14 was increased in surviving patients (p < 0.05). Conclusions The Firmicutes/Bacteroidetes ratio, reflecting overall gut microbial composition, was significantly lower on day three of sepsis diagnosis. Changes in the abundance of specific gut microbiota may serve as prognostic markers in patients with sepsis.https://doi.org/10.1186/s12866-024-03188-6SepsisGut microbiotaMortalityIntensive careOutcomePrediction |
| spellingShingle | Feiyu Luan Yang Zhou Xiaohui Ma Yue Li Yahui Peng Xiaonan Jia Nana Li Xibo Wang Yinghao Luo Mingyin Man Qianqian Zhang Chunying Wang Kaijiang Yu Mingyan Zhao Changsong Wang Gut microbiota composition and changes in patients with sepsis: potential markers for predicting survival BMC Microbiology Sepsis Gut microbiota Mortality Intensive care Outcome Prediction |
| title | Gut microbiota composition and changes in patients with sepsis: potential markers for predicting survival |
| title_full | Gut microbiota composition and changes in patients with sepsis: potential markers for predicting survival |
| title_fullStr | Gut microbiota composition and changes in patients with sepsis: potential markers for predicting survival |
| title_full_unstemmed | Gut microbiota composition and changes in patients with sepsis: potential markers for predicting survival |
| title_short | Gut microbiota composition and changes in patients with sepsis: potential markers for predicting survival |
| title_sort | gut microbiota composition and changes in patients with sepsis potential markers for predicting survival |
| topic | Sepsis Gut microbiota Mortality Intensive care Outcome Prediction |
| url | https://doi.org/10.1186/s12866-024-03188-6 |
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