Ultrasensitive Electrochemical Detection of Clostridium perfringens DNA Based Morphology-Dependent DNA Adsorption Properties of CeO2 Nanorods in Dairy Products
Foodborne pathogens such as Clostridium perfringens can cause diverse illnesses and seriously threaten to human health, yet far less attention has been given to detecting these pathogenic bacteria. Herein, two morphologies of nanoceria were synthesized via adjusting the concentration of NaOH, and Ce...
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MDPI AG
2018-06-01
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| Series: | Sensors |
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| Online Access: | http://www.mdpi.com/1424-8220/18/6/1878 |
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| author | Xingcan Qian Qing Qu Lei Li Xin Ran Limei Zuo Rui Huang Qiang Wang |
| author_facet | Xingcan Qian Qing Qu Lei Li Xin Ran Limei Zuo Rui Huang Qiang Wang |
| author_sort | Xingcan Qian |
| collection | DOAJ |
| description | Foodborne pathogens such as Clostridium perfringens can cause diverse illnesses and seriously threaten to human health, yet far less attention has been given to detecting these pathogenic bacteria. Herein, two morphologies of nanoceria were synthesized via adjusting the concentration of NaOH, and CeO2 nanorod has been utilized as sensing material to achieve sensitive and selective detection of C. perfringens DNA sequence due to its strong adsorption ability towards DNA compared to nanoparticle. The DNA probe was tightly immobilized on CeO2/chitosan modified electrode surface via metal coordination, and the DNA surface density was 2.51 × 10−10 mol/cm2. Under optimal experimental conditions, the electrochemical impedance biosensor displays favorable selectivity toward target DNA in comparison with base-mismatched and non-complementary DNA. The dynamic linear range of the proposed biosensor for detecting oligonucleotide sequence of Clostridium perfringens was from 1.0 × 10−14 to 1.0 × 10−7 mol/L. The detection limit was 7.06 × 10−15 mol/L. In comparison, differential pulse voltammetry (DPV) method quantified the target DNA with a detection limit of 1.95 × 10−15 mol/L. Moreover, the DNA biosensor could detect C. perfringens extracted DNA in dairy products and provided a potential application in food quality control. |
| first_indexed | 2024-12-10T08:06:41Z |
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| institution | Directory Open Access Journal |
| issn | 1424-8220 |
| language | English |
| last_indexed | 2024-12-10T08:06:41Z |
| publishDate | 2018-06-01 |
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| spelling | doaj.art-81d84e9329b04271aa43246e994685792022-12-22T01:56:39ZengMDPI AGSensors1424-82202018-06-01186187810.3390/s18061878s18061878Ultrasensitive Electrochemical Detection of Clostridium perfringens DNA Based Morphology-Dependent DNA Adsorption Properties of CeO2 Nanorods in Dairy ProductsXingcan Qian0Qing Qu1Lei Li2Xin Ran3Limei Zuo4Rui Huang5Qiang Wang6School of Chemical Science and Technology, Yunnan University, Kunming 650091, ChinaSchool of Chemical Science and Technology, Yunnan University, Kunming 650091, ChinaLaboratory for Conservation and Utilization of Bio-Resources, Yunnan University, Kunming 650091, ChinaSchool of Chemical Science and Technology, Yunnan University, Kunming 650091, ChinaSchool of Chemical Science and Technology, Yunnan University, Kunming 650091, ChinaSino-Pipeline International Company Limited, Beijing 100000, ChinaSino-Pipeline International Company Limited, Beijing 100000, ChinaFoodborne pathogens such as Clostridium perfringens can cause diverse illnesses and seriously threaten to human health, yet far less attention has been given to detecting these pathogenic bacteria. Herein, two morphologies of nanoceria were synthesized via adjusting the concentration of NaOH, and CeO2 nanorod has been utilized as sensing material to achieve sensitive and selective detection of C. perfringens DNA sequence due to its strong adsorption ability towards DNA compared to nanoparticle. The DNA probe was tightly immobilized on CeO2/chitosan modified electrode surface via metal coordination, and the DNA surface density was 2.51 × 10−10 mol/cm2. Under optimal experimental conditions, the electrochemical impedance biosensor displays favorable selectivity toward target DNA in comparison with base-mismatched and non-complementary DNA. The dynamic linear range of the proposed biosensor for detecting oligonucleotide sequence of Clostridium perfringens was from 1.0 × 10−14 to 1.0 × 10−7 mol/L. The detection limit was 7.06 × 10−15 mol/L. In comparison, differential pulse voltammetry (DPV) method quantified the target DNA with a detection limit of 1.95 × 10−15 mol/L. Moreover, the DNA biosensor could detect C. perfringens extracted DNA in dairy products and provided a potential application in food quality control.http://www.mdpi.com/1424-8220/18/6/1878label-freeCeO2 nanorodselectrochemical DNA biosensorClostridium perfringens |
| spellingShingle | Xingcan Qian Qing Qu Lei Li Xin Ran Limei Zuo Rui Huang Qiang Wang Ultrasensitive Electrochemical Detection of Clostridium perfringens DNA Based Morphology-Dependent DNA Adsorption Properties of CeO2 Nanorods in Dairy Products Sensors label-free CeO2 nanorods electrochemical DNA biosensor Clostridium perfringens |
| title | Ultrasensitive Electrochemical Detection of Clostridium perfringens DNA Based Morphology-Dependent DNA Adsorption Properties of CeO2 Nanorods in Dairy Products |
| title_full | Ultrasensitive Electrochemical Detection of Clostridium perfringens DNA Based Morphology-Dependent DNA Adsorption Properties of CeO2 Nanorods in Dairy Products |
| title_fullStr | Ultrasensitive Electrochemical Detection of Clostridium perfringens DNA Based Morphology-Dependent DNA Adsorption Properties of CeO2 Nanorods in Dairy Products |
| title_full_unstemmed | Ultrasensitive Electrochemical Detection of Clostridium perfringens DNA Based Morphology-Dependent DNA Adsorption Properties of CeO2 Nanorods in Dairy Products |
| title_short | Ultrasensitive Electrochemical Detection of Clostridium perfringens DNA Based Morphology-Dependent DNA Adsorption Properties of CeO2 Nanorods in Dairy Products |
| title_sort | ultrasensitive electrochemical detection of clostridium perfringens dna based morphology dependent dna adsorption properties of ceo2 nanorods in dairy products |
| topic | label-free CeO2 nanorods electrochemical DNA biosensor Clostridium perfringens |
| url | http://www.mdpi.com/1424-8220/18/6/1878 |
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