The protective effects of lycopus lucidus turcz in diabetic retinopathy and its possible mechanisms
The aim of the present study was to investigate the effect of Lycopus lucidus Turcz (LT) on diabetic retinopathy (DR) and its underlying mechanisms. SD rats and human retinal microvascular endothelial cells (HRECs) were applied for establishment DR model. HE and TUNEL staining were used to evaluate...
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| Format: | Article |
| Language: | English |
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Taylor & Francis Group
2019-12-01
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| Series: | Artificial Cells, Nanomedicine, and Biotechnology |
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| Online Access: | https://www.tandfonline.com/doi/10.1080/21691401.2019.1640230 |
| _version_ | 1811291875422765056 |
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| author | Jinlu Liu Sai Bhuvanagiri Xiaohan Qu |
| author_facet | Jinlu Liu Sai Bhuvanagiri Xiaohan Qu |
| author_sort | Jinlu Liu |
| collection | DOAJ |
| description | The aim of the present study was to investigate the effect of Lycopus lucidus Turcz (LT) on diabetic retinopathy (DR) and its underlying mechanisms. SD rats and human retinal microvascular endothelial cells (HRECs) were applied for establishment DR model. HE and TUNEL staining were used to evaluate the pathological changes and apoptosis of retinal ganglion cells. Additionally, retinal vessels were detected by immunofluorescence staining with CD31 and VEGF. The function of BRB was observed using Evans blue. Moreover, the oxidative stress, inflammation and angiogenesis associated factors were measured respectively. The expression of p38-MAPK/NF-κB signalling proteins were detected by Western blot. The results demonstrated that pathological changes and retinal optic disc cells apoptosis in retinas of diabetic rats, both of which were reduced in the LT-treated group. And LT treatment attenuated the levels of oxidative stress, inflammation and angiogenesis factors. Importantly, the expression levels of p-p38, p-ERK, p-JNK and NF-κB were decreased. After treatment with TNF-α combined with LT, the levels of inflammatory factors were decreased but higher than the negative control. Taken together, the results suggested that LT treatment is of therapeutic benefit by ameliorating oxidative stress, inflammation and angiogenesis of DR via p38-MAPK/NF-κB signaling pathway. |
| first_indexed | 2024-04-13T04:35:56Z |
| format | Article |
| id | doaj.art-81d9b3508af949cba7c96e53c294723c |
| institution | Directory Open Access Journal |
| issn | 2169-1401 2169-141X |
| language | English |
| last_indexed | 2024-04-13T04:35:56Z |
| publishDate | 2019-12-01 |
| publisher | Taylor & Francis Group |
| record_format | Article |
| series | Artificial Cells, Nanomedicine, and Biotechnology |
| spelling | doaj.art-81d9b3508af949cba7c96e53c294723c2022-12-22T03:02:09ZengTaylor & Francis GroupArtificial Cells, Nanomedicine, and Biotechnology2169-14012169-141X2019-12-014712900290810.1080/21691401.2019.1640230The protective effects of lycopus lucidus turcz in diabetic retinopathy and its possible mechanismsJinlu Liu0Sai Bhuvanagiri1Xiaohan Qu2Department of Ophthalmology, The Fourth Affiliated Hospital of China Medical University, Shenyang, ChinaQueens Hospital Center, Mt. Sinai, Icahn School of Medicine, Jamaica, NY, USADepartment of Thoracic Surgery, The First Hospital of China Medical University, Shenyang, ChinaThe aim of the present study was to investigate the effect of Lycopus lucidus Turcz (LT) on diabetic retinopathy (DR) and its underlying mechanisms. SD rats and human retinal microvascular endothelial cells (HRECs) were applied for establishment DR model. HE and TUNEL staining were used to evaluate the pathological changes and apoptosis of retinal ganglion cells. Additionally, retinal vessels were detected by immunofluorescence staining with CD31 and VEGF. The function of BRB was observed using Evans blue. Moreover, the oxidative stress, inflammation and angiogenesis associated factors were measured respectively. The expression of p38-MAPK/NF-κB signalling proteins were detected by Western blot. The results demonstrated that pathological changes and retinal optic disc cells apoptosis in retinas of diabetic rats, both of which were reduced in the LT-treated group. And LT treatment attenuated the levels of oxidative stress, inflammation and angiogenesis factors. Importantly, the expression levels of p-p38, p-ERK, p-JNK and NF-κB were decreased. After treatment with TNF-α combined with LT, the levels of inflammatory factors were decreased but higher than the negative control. Taken together, the results suggested that LT treatment is of therapeutic benefit by ameliorating oxidative stress, inflammation and angiogenesis of DR via p38-MAPK/NF-κB signaling pathway.https://www.tandfonline.com/doi/10.1080/21691401.2019.1640230Diabetic retinopathyinflammationangiogenesisp38NF-κB |
| spellingShingle | Jinlu Liu Sai Bhuvanagiri Xiaohan Qu The protective effects of lycopus lucidus turcz in diabetic retinopathy and its possible mechanisms Artificial Cells, Nanomedicine, and Biotechnology Diabetic retinopathy inflammation angiogenesis p38 NF-κB |
| title | The protective effects of lycopus lucidus turcz in diabetic retinopathy and its possible mechanisms |
| title_full | The protective effects of lycopus lucidus turcz in diabetic retinopathy and its possible mechanisms |
| title_fullStr | The protective effects of lycopus lucidus turcz in diabetic retinopathy and its possible mechanisms |
| title_full_unstemmed | The protective effects of lycopus lucidus turcz in diabetic retinopathy and its possible mechanisms |
| title_short | The protective effects of lycopus lucidus turcz in diabetic retinopathy and its possible mechanisms |
| title_sort | protective effects of lycopus lucidus turcz in diabetic retinopathy and its possible mechanisms |
| topic | Diabetic retinopathy inflammation angiogenesis p38 NF-κB |
| url | https://www.tandfonline.com/doi/10.1080/21691401.2019.1640230 |
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