3D Hierarchical, Nanostructured Chitosan/PLA/HA Scaffolds Doped with TiO<sub>2</sub>/Au/Pt NPs with Tunable Properties for Guided Bone Tissue Engineering
Bone tissue is the second tissue to be replaced. Annually, over four million surgical treatments are performed. Tissue engineering constitutes an alternative to autologous grafts. Its application requires three-dimensional scaffolds, which mimic human body environment. Bone tissue has a highly organ...
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| Format: | Article |
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MDPI AG
2020-04-01
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| Series: | Polymers |
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| Online Access: | https://www.mdpi.com/2073-4360/12/4/792 |
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| author | Julia Radwan-Pragłowska Łukasz Janus Marek Piątkowski Dariusz Bogdał Dalibor Matysek |
| author_facet | Julia Radwan-Pragłowska Łukasz Janus Marek Piątkowski Dariusz Bogdał Dalibor Matysek |
| author_sort | Julia Radwan-Pragłowska |
| collection | DOAJ |
| description | Bone tissue is the second tissue to be replaced. Annually, over four million surgical treatments are performed. Tissue engineering constitutes an alternative to autologous grafts. Its application requires three-dimensional scaffolds, which mimic human body environment. Bone tissue has a highly organized structure and contains mostly inorganic components. The scaffolds of the latest generation should not only be biocompatible but also promote osteoconduction. Poly (lactic acid) nanofibers are commonly used for this purpose; however, they lack bioactivity and do not provide good cell adhesion. Chitosan is a commonly used biopolymer which positively affects osteoblasts’ behavior. The aim of this article was to prepare novel hybrid 3D scaffolds containing nanohydroxyapatite capable of cell-response stimulation. The matrixes were successfully obtained by PLA electrospinning and microwave-assisted chitosan crosslinking, followed by doping with three types of metallic nanoparticles (Au, Pt, and TiO<sub>2</sub>). The products and semi-components were characterized over their physicochemical properties, such as chemical structure, crystallinity, and swelling degree. Nanoparticles’ and ready biomaterials’ morphologies were investigated by SEM and TEM methods. Finally, the scaffolds were studied over bioactivity on MG-63 and effect on current-stimulated biomineralization. Obtained results confirmed preparation of tunable biomimicking matrixes which may be used as a promising tool for bone-tissue engineering. |
| first_indexed | 2024-03-10T20:44:00Z |
| format | Article |
| id | doaj.art-81dd911c43a84e3d9c931e056d45bcd1 |
| institution | Directory Open Access Journal |
| issn | 2073-4360 |
| language | English |
| last_indexed | 2024-03-10T20:44:00Z |
| publishDate | 2020-04-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Polymers |
| spelling | doaj.art-81dd911c43a84e3d9c931e056d45bcd12023-11-19T20:28:47ZengMDPI AGPolymers2073-43602020-04-0112479210.3390/polym120407923D Hierarchical, Nanostructured Chitosan/PLA/HA Scaffolds Doped with TiO<sub>2</sub>/Au/Pt NPs with Tunable Properties for Guided Bone Tissue EngineeringJulia Radwan-Pragłowska0Łukasz Janus1Marek Piątkowski2Dariusz Bogdał3Dalibor Matysek4Department of Physical Chemistry, Faculty of Chemical Engineering and Technology, Cracow University of Technology, 31–155 Cracow, PolandDepartment of Physical Chemistry, Faculty of Chemical Engineering and Technology, Cracow University of Technology, 31–155 Cracow, PolandDepartment of Physical Chemistry, Faculty of Chemical Engineering and Technology, Cracow University of Technology, 31–155 Cracow, PolandDepartment of Physical Chemistry, Faculty of Chemical Engineering and Technology, Cracow University of Technology, 31–155 Cracow, PolandFaculty of Mining and Geology, Technical University of Ostrava; 708 00 Ostrava, Czech RepublicBone tissue is the second tissue to be replaced. Annually, over four million surgical treatments are performed. Tissue engineering constitutes an alternative to autologous grafts. Its application requires three-dimensional scaffolds, which mimic human body environment. Bone tissue has a highly organized structure and contains mostly inorganic components. The scaffolds of the latest generation should not only be biocompatible but also promote osteoconduction. Poly (lactic acid) nanofibers are commonly used for this purpose; however, they lack bioactivity and do not provide good cell adhesion. Chitosan is a commonly used biopolymer which positively affects osteoblasts’ behavior. The aim of this article was to prepare novel hybrid 3D scaffolds containing nanohydroxyapatite capable of cell-response stimulation. The matrixes were successfully obtained by PLA electrospinning and microwave-assisted chitosan crosslinking, followed by doping with three types of metallic nanoparticles (Au, Pt, and TiO<sub>2</sub>). The products and semi-components were characterized over their physicochemical properties, such as chemical structure, crystallinity, and swelling degree. Nanoparticles’ and ready biomaterials’ morphologies were investigated by SEM and TEM methods. Finally, the scaffolds were studied over bioactivity on MG-63 and effect on current-stimulated biomineralization. Obtained results confirmed preparation of tunable biomimicking matrixes which may be used as a promising tool for bone-tissue engineering.https://www.mdpi.com/2073-4360/12/4/792smart hybrid materialsproperties of nanoparticles–reinforced polymersbiotechnology |
| spellingShingle | Julia Radwan-Pragłowska Łukasz Janus Marek Piątkowski Dariusz Bogdał Dalibor Matysek 3D Hierarchical, Nanostructured Chitosan/PLA/HA Scaffolds Doped with TiO<sub>2</sub>/Au/Pt NPs with Tunable Properties for Guided Bone Tissue Engineering Polymers smart hybrid materials properties of nanoparticles–reinforced polymers biotechnology |
| title | 3D Hierarchical, Nanostructured Chitosan/PLA/HA Scaffolds Doped with TiO<sub>2</sub>/Au/Pt NPs with Tunable Properties for Guided Bone Tissue Engineering |
| title_full | 3D Hierarchical, Nanostructured Chitosan/PLA/HA Scaffolds Doped with TiO<sub>2</sub>/Au/Pt NPs with Tunable Properties for Guided Bone Tissue Engineering |
| title_fullStr | 3D Hierarchical, Nanostructured Chitosan/PLA/HA Scaffolds Doped with TiO<sub>2</sub>/Au/Pt NPs with Tunable Properties for Guided Bone Tissue Engineering |
| title_full_unstemmed | 3D Hierarchical, Nanostructured Chitosan/PLA/HA Scaffolds Doped with TiO<sub>2</sub>/Au/Pt NPs with Tunable Properties for Guided Bone Tissue Engineering |
| title_short | 3D Hierarchical, Nanostructured Chitosan/PLA/HA Scaffolds Doped with TiO<sub>2</sub>/Au/Pt NPs with Tunable Properties for Guided Bone Tissue Engineering |
| title_sort | 3d hierarchical nanostructured chitosan pla ha scaffolds doped with tio sub 2 sub au pt nps with tunable properties for guided bone tissue engineering |
| topic | smart hybrid materials properties of nanoparticles–reinforced polymers biotechnology |
| url | https://www.mdpi.com/2073-4360/12/4/792 |
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