Bacteriophage-loaded functional nanofibers for treatment of P. aeruginosa and S. aureus wound infections
Abstract The increasing incidence of infected skin wounds poses a major challenge in clinical practice, especially when conventional antibiotic therapy fails. In this context, bacteriophages emerged as promising alternatives for the treatment of antibiotic-resistant bacteria. However, clinical imple...
| Main Authors: | , , , |
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| Format: | Article |
| Language: | English |
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Nature Portfolio
2023-05-01
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| Series: | Scientific Reports |
| Online Access: | https://doi.org/10.1038/s41598-023-35364-5 |
| _version_ | 1797818072580489216 |
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| author | Tobias Kielholz Felix Rohde Nathalie Jung Maike Windbergs |
| author_facet | Tobias Kielholz Felix Rohde Nathalie Jung Maike Windbergs |
| author_sort | Tobias Kielholz |
| collection | DOAJ |
| description | Abstract The increasing incidence of infected skin wounds poses a major challenge in clinical practice, especially when conventional antibiotic therapy fails. In this context, bacteriophages emerged as promising alternatives for the treatment of antibiotic-resistant bacteria. However, clinical implementation remains hampered by the lack of efficient delivery approaches to infected wound tissue. In this study, bacteriophage-loaded electrospun fiber mats were successfully developed as next-generation wound dressings for the treatment of infected wounds. We employed a coaxial electrospinning approach, creating fibers with a protective polymer shell, enveloping bacteriophages in the core while maintaining their antimicrobial activity. The novel fibers exhibited a reproducible fiber diameter range and morphology, while the mechanical fiber properties were ideal for application onto wounds. Further, immediate release kinetics for the phages were confirmed as well as the biocompatibility of the fibers with human skin cells. Antimicrobial activity was demonstrated against Staphylococcus aureus and Pseudomonas aeruginosa and the core/shell formulation maintained the bacteriophage activity for 4 weeks when stored at − 20 °C. Based on these promising characteristics, our approach holds great potential as a platform technology for the encapsulation of bioactive bacteriophages to enable the translation of phage therapy into clinical application. |
| first_indexed | 2024-03-13T09:02:45Z |
| format | Article |
| id | doaj.art-81debe7208b64b05ad6144ec64ccc8cc |
| institution | Directory Open Access Journal |
| issn | 2045-2322 |
| language | English |
| last_indexed | 2024-03-13T09:02:45Z |
| publishDate | 2023-05-01 |
| publisher | Nature Portfolio |
| record_format | Article |
| series | Scientific Reports |
| spelling | doaj.art-81debe7208b64b05ad6144ec64ccc8cc2023-05-28T11:14:17ZengNature PortfolioScientific Reports2045-23222023-05-0113111310.1038/s41598-023-35364-5Bacteriophage-loaded functional nanofibers for treatment of P. aeruginosa and S. aureus wound infectionsTobias Kielholz0Felix Rohde1Nathalie Jung2Maike Windbergs3Institute of Pharmaceutical Technology and Buchmann Institute for Molecular Life Sciences, Goethe University FrankfurtInstitute of Pharmaceutical Technology and Buchmann Institute for Molecular Life Sciences, Goethe University FrankfurtInstitute of Pharmaceutical Technology and Buchmann Institute for Molecular Life Sciences, Goethe University FrankfurtInstitute of Pharmaceutical Technology and Buchmann Institute for Molecular Life Sciences, Goethe University FrankfurtAbstract The increasing incidence of infected skin wounds poses a major challenge in clinical practice, especially when conventional antibiotic therapy fails. In this context, bacteriophages emerged as promising alternatives for the treatment of antibiotic-resistant bacteria. However, clinical implementation remains hampered by the lack of efficient delivery approaches to infected wound tissue. In this study, bacteriophage-loaded electrospun fiber mats were successfully developed as next-generation wound dressings for the treatment of infected wounds. We employed a coaxial electrospinning approach, creating fibers with a protective polymer shell, enveloping bacteriophages in the core while maintaining their antimicrobial activity. The novel fibers exhibited a reproducible fiber diameter range and morphology, while the mechanical fiber properties were ideal for application onto wounds. Further, immediate release kinetics for the phages were confirmed as well as the biocompatibility of the fibers with human skin cells. Antimicrobial activity was demonstrated against Staphylococcus aureus and Pseudomonas aeruginosa and the core/shell formulation maintained the bacteriophage activity for 4 weeks when stored at − 20 °C. Based on these promising characteristics, our approach holds great potential as a platform technology for the encapsulation of bioactive bacteriophages to enable the translation of phage therapy into clinical application.https://doi.org/10.1038/s41598-023-35364-5 |
| spellingShingle | Tobias Kielholz Felix Rohde Nathalie Jung Maike Windbergs Bacteriophage-loaded functional nanofibers for treatment of P. aeruginosa and S. aureus wound infections Scientific Reports |
| title | Bacteriophage-loaded functional nanofibers for treatment of P. aeruginosa and S. aureus wound infections |
| title_full | Bacteriophage-loaded functional nanofibers for treatment of P. aeruginosa and S. aureus wound infections |
| title_fullStr | Bacteriophage-loaded functional nanofibers for treatment of P. aeruginosa and S. aureus wound infections |
| title_full_unstemmed | Bacteriophage-loaded functional nanofibers for treatment of P. aeruginosa and S. aureus wound infections |
| title_short | Bacteriophage-loaded functional nanofibers for treatment of P. aeruginosa and S. aureus wound infections |
| title_sort | bacteriophage loaded functional nanofibers for treatment of p aeruginosa and s aureus wound infections |
| url | https://doi.org/10.1038/s41598-023-35364-5 |
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