IrrE, a global regulator of extreme radiation resistance in Deinococcus radiodurans, enhances salt tolerance in Escherichia coli and Brassica napus.
BACKGROUND: Globally, about 20% of cultivated land is now affected by salinity. Salt tolerance is a trait of importance to all crops in saline soils. Previous efforts to improve salt tolerance in crop plants have met with only limited success. Bacteria of the genus Deinococcus are known for their ab...
| Main Authors: | , , , , , , , , , , , , , , , , , |
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| Format: | Article |
| Language: | English |
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Public Library of Science (PLoS)
2009-01-01
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| Series: | PLoS ONE |
| Online Access: | http://europepmc.org/articles/PMC2635966?pdf=render |
| _version_ | 1811269814256140288 |
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| author | Jie Pan Jin Wang Zhengfu Zhou Yongliang Yan Wei Zhang Wei Lu Shuzhen Ping Qilin Dai Menglong Yuan Bin Feng Xiaoguang Hou Ying Zhang Ruiqiang Ma Tingting Liu Lu Feng Lei Wang Ming Chen Min Lin |
| author_facet | Jie Pan Jin Wang Zhengfu Zhou Yongliang Yan Wei Zhang Wei Lu Shuzhen Ping Qilin Dai Menglong Yuan Bin Feng Xiaoguang Hou Ying Zhang Ruiqiang Ma Tingting Liu Lu Feng Lei Wang Ming Chen Min Lin |
| author_sort | Jie Pan |
| collection | DOAJ |
| description | BACKGROUND: Globally, about 20% of cultivated land is now affected by salinity. Salt tolerance is a trait of importance to all crops in saline soils. Previous efforts to improve salt tolerance in crop plants have met with only limited success. Bacteria of the genus Deinococcus are known for their ability to survive highly stressful conditions, and therefore possess a unique pool of genes conferring extreme resistance. In Deinococcus radiodurans, the irrE gene encodes a global regulator responsible for extreme radioresistance. METHODOLOGY/PRINCIPAL FINDINGS: Using plate assays, we showed that IrrE protected E. coli cells against salt shock and other abiotic stresses such as oxidative, osmotic and thermal shocks. Comparative proteomic analysis revealed that IrrE functions as a switch to regulate different sets of proteins such as stress responsive proteins, protein kinases, glycerol-degrading enzymes, detoxification proteins, and growth-related proteins in E. coli. We also used quantitative RT-PCR to investigate expression of nine selected stress-responsive genes in transgenic and wild-type Brassica napus plants. Transgenic B. napus plants expressing the IrrE protein can tolerate 350 mM NaCl, a concentration that inhibits the growth of almost all crop plants. CONCLUSIONS: Expression of IrrE, a global regulator for extreme radiation resistance in D. radiodurans, confers significantly enhanced salt tolerance in both E. coli and B. napus. We thus propose that the irrE gene might be used as a potentially promising transgene to improve abiotic stress tolerances in crop plants. |
| first_indexed | 2024-04-12T21:48:42Z |
| format | Article |
| id | doaj.art-81dee1fa0b68496680d3cba2ec64eab6 |
| institution | Directory Open Access Journal |
| issn | 1932-6203 |
| language | English |
| last_indexed | 2024-04-12T21:48:42Z |
| publishDate | 2009-01-01 |
| publisher | Public Library of Science (PLoS) |
| record_format | Article |
| series | PLoS ONE |
| spelling | doaj.art-81dee1fa0b68496680d3cba2ec64eab62022-12-22T03:15:33ZengPublic Library of Science (PLoS)PLoS ONE1932-62032009-01-0142e442210.1371/journal.pone.0004422IrrE, a global regulator of extreme radiation resistance in Deinococcus radiodurans, enhances salt tolerance in Escherichia coli and Brassica napus.Jie PanJin WangZhengfu ZhouYongliang YanWei ZhangWei LuShuzhen PingQilin DaiMenglong YuanBin FengXiaoguang HouYing ZhangRuiqiang MaTingting LiuLu FengLei WangMing ChenMin LinBACKGROUND: Globally, about 20% of cultivated land is now affected by salinity. Salt tolerance is a trait of importance to all crops in saline soils. Previous efforts to improve salt tolerance in crop plants have met with only limited success. Bacteria of the genus Deinococcus are known for their ability to survive highly stressful conditions, and therefore possess a unique pool of genes conferring extreme resistance. In Deinococcus radiodurans, the irrE gene encodes a global regulator responsible for extreme radioresistance. METHODOLOGY/PRINCIPAL FINDINGS: Using plate assays, we showed that IrrE protected E. coli cells against salt shock and other abiotic stresses such as oxidative, osmotic and thermal shocks. Comparative proteomic analysis revealed that IrrE functions as a switch to regulate different sets of proteins such as stress responsive proteins, protein kinases, glycerol-degrading enzymes, detoxification proteins, and growth-related proteins in E. coli. We also used quantitative RT-PCR to investigate expression of nine selected stress-responsive genes in transgenic and wild-type Brassica napus plants. Transgenic B. napus plants expressing the IrrE protein can tolerate 350 mM NaCl, a concentration that inhibits the growth of almost all crop plants. CONCLUSIONS: Expression of IrrE, a global regulator for extreme radiation resistance in D. radiodurans, confers significantly enhanced salt tolerance in both E. coli and B. napus. We thus propose that the irrE gene might be used as a potentially promising transgene to improve abiotic stress tolerances in crop plants.http://europepmc.org/articles/PMC2635966?pdf=render |
| spellingShingle | Jie Pan Jin Wang Zhengfu Zhou Yongliang Yan Wei Zhang Wei Lu Shuzhen Ping Qilin Dai Menglong Yuan Bin Feng Xiaoguang Hou Ying Zhang Ruiqiang Ma Tingting Liu Lu Feng Lei Wang Ming Chen Min Lin IrrE, a global regulator of extreme radiation resistance in Deinococcus radiodurans, enhances salt tolerance in Escherichia coli and Brassica napus. PLoS ONE |
| title | IrrE, a global regulator of extreme radiation resistance in Deinococcus radiodurans, enhances salt tolerance in Escherichia coli and Brassica napus. |
| title_full | IrrE, a global regulator of extreme radiation resistance in Deinococcus radiodurans, enhances salt tolerance in Escherichia coli and Brassica napus. |
| title_fullStr | IrrE, a global regulator of extreme radiation resistance in Deinococcus radiodurans, enhances salt tolerance in Escherichia coli and Brassica napus. |
| title_full_unstemmed | IrrE, a global regulator of extreme radiation resistance in Deinococcus radiodurans, enhances salt tolerance in Escherichia coli and Brassica napus. |
| title_short | IrrE, a global regulator of extreme radiation resistance in Deinococcus radiodurans, enhances salt tolerance in Escherichia coli and Brassica napus. |
| title_sort | irre a global regulator of extreme radiation resistance in deinococcus radiodurans enhances salt tolerance in escherichia coli and brassica napus |
| url | http://europepmc.org/articles/PMC2635966?pdf=render |
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