<i>Listeria monocytogenes</i> Wall Teichoic Acid Glycosylation Promotes Surface Anchoring of Virulence Factors, Resistance to Antimicrobial Peptides, and Decreased Susceptibility to Antibiotics
The cell wall of <i>Listeria monocytogenes</i> (<i>Lm</i>)<i>,</i> a major intracellular foodborne bacterial pathogen, comprises a thick peptidoglycan layer that serves as a scaffold for glycopolymers such as wall teichoic acids (WTAs). WTAs contain non-essential...
| Main Authors: | , , , , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
MDPI AG
2020-04-01
|
| Series: | Pathogens |
| Subjects: | |
| Online Access: | https://www.mdpi.com/2076-0817/9/4/290 |
| _version_ | 1797570487355703296 |
|---|---|
| author | Diana Meireles Rita Pombinho Filipe Carvalho Sandra Sousa Didier Cabanes |
| author_facet | Diana Meireles Rita Pombinho Filipe Carvalho Sandra Sousa Didier Cabanes |
| author_sort | Diana Meireles |
| collection | DOAJ |
| description | The cell wall of <i>Listeria monocytogenes</i> (<i>Lm</i>)<i>,</i> a major intracellular foodborne bacterial pathogen, comprises a thick peptidoglycan layer that serves as a scaffold for glycopolymers such as wall teichoic acids (WTAs). WTAs contain non-essential sugar substituents whose absence prevents bacteriophage binding and impacts antigenicity, sensitivity to antimicrobials, and virulence. Here, we demonstrated, for the first time, the triple function of <i>Lm</i> WTA glycosylations in the following: (1) supporting the correct anchoring of major <i>Lm</i> virulence factors at the bacterial surface, namely Ami and InlB; (2) promoting <i>Lm</i> resistance to antimicrobial peptides (AMPs); and (3) decreasing <i>Lm</i> sensitivity to some antibiotics. We showed that while the decoration of WTAs by rhamnose in <i>Lm</i> serovar 1/2a and by galactose in serovar 4b are important for the surface anchoring of Ami and InlB, N-acetylglucosamine in serovar 1/2a and glucose in serovar 4b are dispensable for the surface association of InlB or InlB/Ami. We found that the absence of a single glycosylation only had a slight impact on the sensibility of <i>Lm</i> to AMPs and antibiotics, however the concomitant deficiency of both glycosylations (rhamnose and <i>N</i>-acetylglucosamine in serovar 1/2a, and galactose and glucose in serovar 4b) significantly impaired the <i>Lm</i> capacity to overcome the action of antimicrobials. We propose WTA glycosylation as a broad mechanism used by <i>Lm,</i> not only to properly anchor surface virulence factors, but also to resist AMPs and antibiotics. WTA glycosyltransferases thus emerge as promising drug targets to attenuate the virulence of bacterial pathogens, while increasing their susceptibility to host immune defenses and potentiating the action of antibiotics. |
| first_indexed | 2024-03-10T20:26:14Z |
| format | Article |
| id | doaj.art-81e0030c1d994592a2ec85dda5c3dc40 |
| institution | Directory Open Access Journal |
| issn | 2076-0817 |
| language | English |
| last_indexed | 2024-03-10T20:26:14Z |
| publishDate | 2020-04-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Pathogens |
| spelling | doaj.art-81e0030c1d994592a2ec85dda5c3dc402023-11-19T21:46:53ZengMDPI AGPathogens2076-08172020-04-019429010.3390/pathogens9040290<i>Listeria monocytogenes</i> Wall Teichoic Acid Glycosylation Promotes Surface Anchoring of Virulence Factors, Resistance to Antimicrobial Peptides, and Decreased Susceptibility to AntibioticsDiana Meireles0Rita Pombinho1Filipe Carvalho2Sandra Sousa3Didier Cabanes4i3S–Instituto de Investigação e Inovação em Saúde, Universidade do Porto, 4200-135 Porto, Portugali3S–Instituto de Investigação e Inovação em Saúde, Universidade do Porto, 4200-135 Porto, Portugali3S–Instituto de Investigação e Inovação em Saúde, Universidade do Porto, 4200-135 Porto, Portugali3S–Instituto de Investigação e Inovação em Saúde, Universidade do Porto, 4200-135 Porto, Portugali3S–Instituto de Investigação e Inovação em Saúde, Universidade do Porto, 4200-135 Porto, PortugalThe cell wall of <i>Listeria monocytogenes</i> (<i>Lm</i>)<i>,</i> a major intracellular foodborne bacterial pathogen, comprises a thick peptidoglycan layer that serves as a scaffold for glycopolymers such as wall teichoic acids (WTAs). WTAs contain non-essential sugar substituents whose absence prevents bacteriophage binding and impacts antigenicity, sensitivity to antimicrobials, and virulence. Here, we demonstrated, for the first time, the triple function of <i>Lm</i> WTA glycosylations in the following: (1) supporting the correct anchoring of major <i>Lm</i> virulence factors at the bacterial surface, namely Ami and InlB; (2) promoting <i>Lm</i> resistance to antimicrobial peptides (AMPs); and (3) decreasing <i>Lm</i> sensitivity to some antibiotics. We showed that while the decoration of WTAs by rhamnose in <i>Lm</i> serovar 1/2a and by galactose in serovar 4b are important for the surface anchoring of Ami and InlB, N-acetylglucosamine in serovar 1/2a and glucose in serovar 4b are dispensable for the surface association of InlB or InlB/Ami. We found that the absence of a single glycosylation only had a slight impact on the sensibility of <i>Lm</i> to AMPs and antibiotics, however the concomitant deficiency of both glycosylations (rhamnose and <i>N</i>-acetylglucosamine in serovar 1/2a, and galactose and glucose in serovar 4b) significantly impaired the <i>Lm</i> capacity to overcome the action of antimicrobials. We propose WTA glycosylation as a broad mechanism used by <i>Lm,</i> not only to properly anchor surface virulence factors, but also to resist AMPs and antibiotics. WTA glycosyltransferases thus emerge as promising drug targets to attenuate the virulence of bacterial pathogens, while increasing their susceptibility to host immune defenses and potentiating the action of antibiotics.https://www.mdpi.com/2076-0817/9/4/290wall teichoic acid glycosylationglycosyltransferaseGram-positive pathogensantimicrobial peptidesantibiotics |
| spellingShingle | Diana Meireles Rita Pombinho Filipe Carvalho Sandra Sousa Didier Cabanes <i>Listeria monocytogenes</i> Wall Teichoic Acid Glycosylation Promotes Surface Anchoring of Virulence Factors, Resistance to Antimicrobial Peptides, and Decreased Susceptibility to Antibiotics Pathogens wall teichoic acid glycosylation glycosyltransferase Gram-positive pathogens antimicrobial peptides antibiotics |
| title | <i>Listeria monocytogenes</i> Wall Teichoic Acid Glycosylation Promotes Surface Anchoring of Virulence Factors, Resistance to Antimicrobial Peptides, and Decreased Susceptibility to Antibiotics |
| title_full | <i>Listeria monocytogenes</i> Wall Teichoic Acid Glycosylation Promotes Surface Anchoring of Virulence Factors, Resistance to Antimicrobial Peptides, and Decreased Susceptibility to Antibiotics |
| title_fullStr | <i>Listeria monocytogenes</i> Wall Teichoic Acid Glycosylation Promotes Surface Anchoring of Virulence Factors, Resistance to Antimicrobial Peptides, and Decreased Susceptibility to Antibiotics |
| title_full_unstemmed | <i>Listeria monocytogenes</i> Wall Teichoic Acid Glycosylation Promotes Surface Anchoring of Virulence Factors, Resistance to Antimicrobial Peptides, and Decreased Susceptibility to Antibiotics |
| title_short | <i>Listeria monocytogenes</i> Wall Teichoic Acid Glycosylation Promotes Surface Anchoring of Virulence Factors, Resistance to Antimicrobial Peptides, and Decreased Susceptibility to Antibiotics |
| title_sort | i listeria monocytogenes i wall teichoic acid glycosylation promotes surface anchoring of virulence factors resistance to antimicrobial peptides and decreased susceptibility to antibiotics |
| topic | wall teichoic acid glycosylation glycosyltransferase Gram-positive pathogens antimicrobial peptides antibiotics |
| url | https://www.mdpi.com/2076-0817/9/4/290 |
| work_keys_str_mv | AT dianameireles ilisteriamonocytogenesiwallteichoicacidglycosylationpromotessurfaceanchoringofvirulencefactorsresistancetoantimicrobialpeptidesanddecreasedsusceptibilitytoantibiotics AT ritapombinho ilisteriamonocytogenesiwallteichoicacidglycosylationpromotessurfaceanchoringofvirulencefactorsresistancetoantimicrobialpeptidesanddecreasedsusceptibilitytoantibiotics AT filipecarvalho ilisteriamonocytogenesiwallteichoicacidglycosylationpromotessurfaceanchoringofvirulencefactorsresistancetoantimicrobialpeptidesanddecreasedsusceptibilitytoantibiotics AT sandrasousa ilisteriamonocytogenesiwallteichoicacidglycosylationpromotessurfaceanchoringofvirulencefactorsresistancetoantimicrobialpeptidesanddecreasedsusceptibilitytoantibiotics AT didiercabanes ilisteriamonocytogenesiwallteichoicacidglycosylationpromotessurfaceanchoringofvirulencefactorsresistancetoantimicrobialpeptidesanddecreasedsusceptibilitytoantibiotics |