Expression and function of myelin expression factor 2 in hepatocellular carcinoma
Abstract Introduction Hepatocellular carcinoma (HCC) is one of the most common malignant tumours in the world and has a high mortality rate. However, the pathogenesis of HCC remains unclear. This study aimed to investigate the potential biomarkers of HCC. Methods ONCOMINE, HCCDB and THE HUMAN PROTEI...
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| Format: | Article |
| Language: | English |
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BMC
2023-01-01
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| Series: | BMC Gastroenterology |
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| Online Access: | https://doi.org/10.1186/s12876-023-02644-3 |
| _version_ | 1797945885171122176 |
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| author | Peng Zhang Jiang-Hua Zhao Lin Chen Zhao-Lian Bian Lin-Ling Ju Hui-Xuan Wang Wei-Hua Cai |
| author_facet | Peng Zhang Jiang-Hua Zhao Lin Chen Zhao-Lian Bian Lin-Ling Ju Hui-Xuan Wang Wei-Hua Cai |
| author_sort | Peng Zhang |
| collection | DOAJ |
| description | Abstract Introduction Hepatocellular carcinoma (HCC) is one of the most common malignant tumours in the world and has a high mortality rate. However, the pathogenesis of HCC remains unclear. This study aimed to investigate the potential biomarkers of HCC. Methods ONCOMINE, HCCDB and THE HUMAN PROTEIN ATLAS were used to identify myelin expression factor 2 (MYEF2) as a potential biomarker for HCC. The Cancer Genome Atlas database was used to further validate and analyse the value of MYEF2. Kaplan–Meier Plotter was used for the prognostic analysis. The COX regression model and Kaplan–Meier method were used to investigate the clinical value of MYEF2 in the prognosis of HCC by reviewing the survival status of patients. Fluorescent quantitative polymerase chain reaction (qPCR) and immunohistochemistry were used to detect the expressions of the MYEF2 mRNA and protein in HCC tissues and cell lines. qPCR and Western blotting were used to validate the efficiency of MYEF2 knockout and overexpression in HCC cells. The invasion and migration abilities regulated by MYEF2 were detected by performing transwell and wound healing assays. Results MYEF2 is significantly upregulated in HCC and is mainly located in the nucleus of HCC cells. MYEF2 expression is significantly associated with the tumour stage, histological grade and TNM stage. High MYEF2 expression is an independent prognostic factor for patients with HCC. Functionally, elevated MYEF2 facilitated cell migration and invasion in vitro. In contrast, decreased MYEF2 inhibited cell migration and invasion. Conclusions MYEF2 may be a novel biomarker with potential diagnosis and prognosis values and as a potential therapeutic target for HCC. |
| first_indexed | 2024-04-10T21:02:13Z |
| format | Article |
| id | doaj.art-81e2820fdbb449fe8f45158889de8db7 |
| institution | Directory Open Access Journal |
| issn | 1471-230X |
| language | English |
| last_indexed | 2024-04-10T21:02:13Z |
| publishDate | 2023-01-01 |
| publisher | BMC |
| record_format | Article |
| series | BMC Gastroenterology |
| spelling | doaj.art-81e2820fdbb449fe8f45158889de8db72023-01-22T12:15:26ZengBMCBMC Gastroenterology1471-230X2023-01-0123111310.1186/s12876-023-02644-3Expression and function of myelin expression factor 2 in hepatocellular carcinomaPeng Zhang0Jiang-Hua Zhao1Lin Chen2Zhao-Lian Bian3Lin-Ling Ju4Hui-Xuan Wang5Wei-Hua Cai6Medical School of Nantong University, Nantong Third People’s HospitalMedical School of Nantong University, Nantong Third People’s HospitalNantong Institute of Liver Diseases, Nantong Third People’s Hospital, Nantong UniversityNantong Institute of Liver Diseases, Nantong Third People’s Hospital, Nantong UniversityNantong Institute of Liver Diseases, Nantong Third People’s Hospital, Nantong UniversityNantong Institute of Liver Diseases, Nantong Third People’s Hospital, Nantong UniversityNantong Institute of Liver Disease, Department of Hepatobiliary Surgery, Nantong Third People’s Hospital, Nantong UniversityAbstract Introduction Hepatocellular carcinoma (HCC) is one of the most common malignant tumours in the world and has a high mortality rate. However, the pathogenesis of HCC remains unclear. This study aimed to investigate the potential biomarkers of HCC. Methods ONCOMINE, HCCDB and THE HUMAN PROTEIN ATLAS were used to identify myelin expression factor 2 (MYEF2) as a potential biomarker for HCC. The Cancer Genome Atlas database was used to further validate and analyse the value of MYEF2. Kaplan–Meier Plotter was used for the prognostic analysis. The COX regression model and Kaplan–Meier method were used to investigate the clinical value of MYEF2 in the prognosis of HCC by reviewing the survival status of patients. Fluorescent quantitative polymerase chain reaction (qPCR) and immunohistochemistry were used to detect the expressions of the MYEF2 mRNA and protein in HCC tissues and cell lines. qPCR and Western blotting were used to validate the efficiency of MYEF2 knockout and overexpression in HCC cells. The invasion and migration abilities regulated by MYEF2 were detected by performing transwell and wound healing assays. Results MYEF2 is significantly upregulated in HCC and is mainly located in the nucleus of HCC cells. MYEF2 expression is significantly associated with the tumour stage, histological grade and TNM stage. High MYEF2 expression is an independent prognostic factor for patients with HCC. Functionally, elevated MYEF2 facilitated cell migration and invasion in vitro. In contrast, decreased MYEF2 inhibited cell migration and invasion. Conclusions MYEF2 may be a novel biomarker with potential diagnosis and prognosis values and as a potential therapeutic target for HCC.https://doi.org/10.1186/s12876-023-02644-3Hepatocellular carcinomaMYEF2PrognosisMigrationInvasion |
| spellingShingle | Peng Zhang Jiang-Hua Zhao Lin Chen Zhao-Lian Bian Lin-Ling Ju Hui-Xuan Wang Wei-Hua Cai Expression and function of myelin expression factor 2 in hepatocellular carcinoma BMC Gastroenterology Hepatocellular carcinoma MYEF2 Prognosis Migration Invasion |
| title | Expression and function of myelin expression factor 2 in hepatocellular carcinoma |
| title_full | Expression and function of myelin expression factor 2 in hepatocellular carcinoma |
| title_fullStr | Expression and function of myelin expression factor 2 in hepatocellular carcinoma |
| title_full_unstemmed | Expression and function of myelin expression factor 2 in hepatocellular carcinoma |
| title_short | Expression and function of myelin expression factor 2 in hepatocellular carcinoma |
| title_sort | expression and function of myelin expression factor 2 in hepatocellular carcinoma |
| topic | Hepatocellular carcinoma MYEF2 Prognosis Migration Invasion |
| url | https://doi.org/10.1186/s12876-023-02644-3 |
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