First successful production of adult corals derived from cryopreserved larvae
Coral reefs worldwide are declining due to increasing concentrations of greenhouse gases, which, combined with local anthropogenic pressure, are exacerbating unprecedented mass coral bleaching. For corals to survive, restoring coral reefs through cryopreservation is crucial. The aim of this study wa...
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| Format: | Article |
| Language: | English |
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Frontiers Media S.A.
2023-05-01
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| Series: | Frontiers in Marine Science |
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| Online Access: | https://www.frontiersin.org/articles/10.3389/fmars.2023.1172102/full |
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| author | Arah Narida Arah Narida Sujune Tsai Wen-Chung Hsieh Zhi-Hong Wen Li-Hsueh Wang Li-Hsueh Wang Cheng-Liang Huang Chiahsin Lin Chiahsin Lin |
| author_facet | Arah Narida Arah Narida Sujune Tsai Wen-Chung Hsieh Zhi-Hong Wen Li-Hsueh Wang Li-Hsueh Wang Cheng-Liang Huang Chiahsin Lin Chiahsin Lin |
| author_sort | Arah Narida |
| collection | DOAJ |
| description | Coral reefs worldwide are declining due to increasing concentrations of greenhouse gases, which, combined with local anthropogenic pressure, are exacerbating unprecedented mass coral bleaching. For corals to survive, restoring coral reefs through cryopreservation is crucial. The aim of this study was to vitrify and laser-warm Stylophora pistillata planulae to allow for feasible settlement, post-settlement survival, and the production of adult corals. The no-observed-effect concentrations were used to determine the best cryoprotective agents for S. pistillata. The larvae were then subjected to cooling and nanolaser warming (300 V, 10 ms pulse width, 2 mm beam diameter) by using two vitrification solutions (VSs; VS1: 2 M dimethyl sulfoxide and 1 M ethylene glycol and VS2: 2M EG and 1M DMSO and gold nanoparticles. The results revealed that VS1-treated larvae had a higher vitrification rate (65%), swimming rate (23.1%), settlement rate (11.54%), and post settlement survival rate (11.54%) than those treated with VS2. Seasonal variations also affected the cryopreservation of the planulae; VS1 was more favorable for the planulae in spring than in fall. Although laser-warmed larvae developed slower morphologically than their controlled counterparts, the production of adult S. pistillata corals from cryopreserved larvae was achieved. The proposed technique can improve the cryopreservation of corals and advance efforts to protect endangered coral species. |
| first_indexed | 2024-03-13T07:03:29Z |
| format | Article |
| id | doaj.art-81e2caf19f6b49089e589d277a977db1 |
| institution | Directory Open Access Journal |
| issn | 2296-7745 |
| language | English |
| last_indexed | 2024-03-13T07:03:29Z |
| publishDate | 2023-05-01 |
| publisher | Frontiers Media S.A. |
| record_format | Article |
| series | Frontiers in Marine Science |
| spelling | doaj.art-81e2caf19f6b49089e589d277a977db12023-06-06T15:33:56ZengFrontiers Media S.A.Frontiers in Marine Science2296-77452023-05-011010.3389/fmars.2023.11721021172102First successful production of adult corals derived from cryopreserved larvaeArah Narida0Arah Narida1Sujune Tsai2Wen-Chung Hsieh3Zhi-Hong Wen4Li-Hsueh Wang5Li-Hsueh Wang6Cheng-Liang Huang7Chiahsin Lin8Chiahsin Lin9Department of Marine Biotechnology and Resources, National Sun Yat-sen University, Kaohsiung, TaiwanNational Museum of Marine Biology & Aquarium, Pingtung, TaiwanDepartment of Post Modern Agriculture, Mingdao University, Changhua, TaiwanHe Wei Precision Company Limited, Hsinchu, TaiwanDepartment of Marine Biotechnology and Resources, National Sun Yat-sen University, Kaohsiung, TaiwanDepartment of Marine Biotechnology and Resources, National Sun Yat-sen University, Kaohsiung, TaiwanNational Museum of Marine Biology & Aquarium, Pingtung, TaiwanDepartment of Applied Chemistry, National Chiayi University, Chiayi, TaiwanNational Museum of Marine Biology & Aquarium, Pingtung, TaiwanGraduate Institute of Marine Biology, National Dong Hwa University, Pingtung, TaiwanCoral reefs worldwide are declining due to increasing concentrations of greenhouse gases, which, combined with local anthropogenic pressure, are exacerbating unprecedented mass coral bleaching. For corals to survive, restoring coral reefs through cryopreservation is crucial. The aim of this study was to vitrify and laser-warm Stylophora pistillata planulae to allow for feasible settlement, post-settlement survival, and the production of adult corals. The no-observed-effect concentrations were used to determine the best cryoprotective agents for S. pistillata. The larvae were then subjected to cooling and nanolaser warming (300 V, 10 ms pulse width, 2 mm beam diameter) by using two vitrification solutions (VSs; VS1: 2 M dimethyl sulfoxide and 1 M ethylene glycol and VS2: 2M EG and 1M DMSO and gold nanoparticles. The results revealed that VS1-treated larvae had a higher vitrification rate (65%), swimming rate (23.1%), settlement rate (11.54%), and post settlement survival rate (11.54%) than those treated with VS2. Seasonal variations also affected the cryopreservation of the planulae; VS1 was more favorable for the planulae in spring than in fall. Although laser-warmed larvae developed slower morphologically than their controlled counterparts, the production of adult S. pistillata corals from cryopreserved larvae was achieved. The proposed technique can improve the cryopreservation of corals and advance efforts to protect endangered coral species.https://www.frontiersin.org/articles/10.3389/fmars.2023.1172102/fullStylophora pistillatacryopreservationlaser warminglarvaeseason |
| spellingShingle | Arah Narida Arah Narida Sujune Tsai Wen-Chung Hsieh Zhi-Hong Wen Li-Hsueh Wang Li-Hsueh Wang Cheng-Liang Huang Chiahsin Lin Chiahsin Lin First successful production of adult corals derived from cryopreserved larvae Frontiers in Marine Science Stylophora pistillata cryopreservation laser warming larvae season |
| title | First successful production of adult corals derived from cryopreserved larvae |
| title_full | First successful production of adult corals derived from cryopreserved larvae |
| title_fullStr | First successful production of adult corals derived from cryopreserved larvae |
| title_full_unstemmed | First successful production of adult corals derived from cryopreserved larvae |
| title_short | First successful production of adult corals derived from cryopreserved larvae |
| title_sort | first successful production of adult corals derived from cryopreserved larvae |
| topic | Stylophora pistillata cryopreservation laser warming larvae season |
| url | https://www.frontiersin.org/articles/10.3389/fmars.2023.1172102/full |
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