<i>Bna.EPF2</i> Enhances Drought Tolerance by Regulating Stomatal Development and Stomatal Size in <i>Brassica napus</i>
Drought stress severely affects global plant growth and production. The enhancement of plant water-use efficiency (WUE) and drought tolerance by the manipulation of the stomata is an effective strategy to deal with water shortage. However, increasing the WUE and drought tolerance by manipulation on...
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| Format: | Article |
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MDPI AG
2023-04-01
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| Series: | International Journal of Molecular Sciences |
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| Online Access: | https://www.mdpi.com/1422-0067/24/9/8007 |
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| author | Peipei Jiao Yuanlin Liang Shaoping Chen Yang Yuan Yongqiang Chen Honghong Hu |
| author_facet | Peipei Jiao Yuanlin Liang Shaoping Chen Yang Yuan Yongqiang Chen Honghong Hu |
| author_sort | Peipei Jiao |
| collection | DOAJ |
| description | Drought stress severely affects global plant growth and production. The enhancement of plant water-use efficiency (WUE) and drought tolerance by the manipulation of the stomata is an effective strategy to deal with water shortage. However, increasing the WUE and drought tolerance by manipulation on the stomata has rarely been tested in <i>Brassica napus</i>. Here, we isolated <i>Bna.EPF2</i>, an epidermal patterning factor (<i>EPF</i>) in <i>Brassica napus</i> (ecotype Westar), and identified its role in drought performance. <i>Bna.EPF2</i> overexpression lines had a reduction average of 19.02% in abaxial stomatal density and smaller stomatal pore size, leading to approximately 25% lower transpiration, which finally resulted in greater instantaneous WUE and enhanced drought tolerance. Interestingly, the reduction in stomatal density did not affect the CO<sub>2</sub> assimilation or yield-related agronomic traits in <i>Bna.EPF2</i> overexpression plants. Together with the complementation of <i>Bna.EPF2</i> significantly decreasing the stomatal density of Arabidopsis <i>epf2</i>, and <i>Bna.EPF2</i> being expressed in mature guard cells, these results suggest that <i>Bna.EPF2</i> not only functions in stomatal density development, but also in stomatal dimension in Brassicas. Taken together, our results suggest that <i>Bna.EPF2</i> improves WUE and drought tolerance by the regulation of stomatal density and stomatal size in Brassica without growth and yield penalty, and provide insight into the manipulation of this gene in the breeding of drought tolerant plants with increased production under water deficit conditions. |
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| issn | 1661-6596 1422-0067 |
| language | English |
| last_indexed | 2024-03-11T04:17:11Z |
| publishDate | 2023-04-01 |
| publisher | MDPI AG |
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| series | International Journal of Molecular Sciences |
| spelling | doaj.art-81e47b1d365247f7b7511981c6537e862023-11-17T23:03:38ZengMDPI AGInternational Journal of Molecular Sciences1661-65961422-00672023-04-01249800710.3390/ijms24098007<i>Bna.EPF2</i> Enhances Drought Tolerance by Regulating Stomatal Development and Stomatal Size in <i>Brassica napus</i>Peipei Jiao0Yuanlin Liang1Shaoping Chen2Yang Yuan3Yongqiang Chen4Honghong Hu5Key Laboratory of Crop Genetic Improvement, Hubei Hongshan Laboratory, Huazhong Agricultural University, Wuhan 430070, ChinaKey Laboratory of Crop Genetic Improvement, Hubei Hongshan Laboratory, Huazhong Agricultural University, Wuhan 430070, ChinaKey Laboratory of Crop Genetic Improvement, Hubei Hongshan Laboratory, Huazhong Agricultural University, Wuhan 430070, ChinaKey Laboratory of Crop Genetic Improvement, Hubei Hongshan Laboratory, Huazhong Agricultural University, Wuhan 430070, ChinaKey Laboratory of Crop Genetic Improvement, Hubei Hongshan Laboratory, Huazhong Agricultural University, Wuhan 430070, ChinaKey Laboratory of Crop Genetic Improvement, Hubei Hongshan Laboratory, Huazhong Agricultural University, Wuhan 430070, ChinaDrought stress severely affects global plant growth and production. The enhancement of plant water-use efficiency (WUE) and drought tolerance by the manipulation of the stomata is an effective strategy to deal with water shortage. However, increasing the WUE and drought tolerance by manipulation on the stomata has rarely been tested in <i>Brassica napus</i>. Here, we isolated <i>Bna.EPF2</i>, an epidermal patterning factor (<i>EPF</i>) in <i>Brassica napus</i> (ecotype Westar), and identified its role in drought performance. <i>Bna.EPF2</i> overexpression lines had a reduction average of 19.02% in abaxial stomatal density and smaller stomatal pore size, leading to approximately 25% lower transpiration, which finally resulted in greater instantaneous WUE and enhanced drought tolerance. Interestingly, the reduction in stomatal density did not affect the CO<sub>2</sub> assimilation or yield-related agronomic traits in <i>Bna.EPF2</i> overexpression plants. Together with the complementation of <i>Bna.EPF2</i> significantly decreasing the stomatal density of Arabidopsis <i>epf2</i>, and <i>Bna.EPF2</i> being expressed in mature guard cells, these results suggest that <i>Bna.EPF2</i> not only functions in stomatal density development, but also in stomatal dimension in Brassicas. Taken together, our results suggest that <i>Bna.EPF2</i> improves WUE and drought tolerance by the regulation of stomatal density and stomatal size in Brassica without growth and yield penalty, and provide insight into the manipulation of this gene in the breeding of drought tolerant plants with increased production under water deficit conditions.https://www.mdpi.com/1422-0067/24/9/8007<i>Bna.EPF2</i><i>Brassica napus</i>drought tolerancestomatal densitystomatal dimensionwater use efficiency |
| spellingShingle | Peipei Jiao Yuanlin Liang Shaoping Chen Yang Yuan Yongqiang Chen Honghong Hu <i>Bna.EPF2</i> Enhances Drought Tolerance by Regulating Stomatal Development and Stomatal Size in <i>Brassica napus</i> International Journal of Molecular Sciences <i>Bna.EPF2</i> <i>Brassica napus</i> drought tolerance stomatal density stomatal dimension water use efficiency |
| title | <i>Bna.EPF2</i> Enhances Drought Tolerance by Regulating Stomatal Development and Stomatal Size in <i>Brassica napus</i> |
| title_full | <i>Bna.EPF2</i> Enhances Drought Tolerance by Regulating Stomatal Development and Stomatal Size in <i>Brassica napus</i> |
| title_fullStr | <i>Bna.EPF2</i> Enhances Drought Tolerance by Regulating Stomatal Development and Stomatal Size in <i>Brassica napus</i> |
| title_full_unstemmed | <i>Bna.EPF2</i> Enhances Drought Tolerance by Regulating Stomatal Development and Stomatal Size in <i>Brassica napus</i> |
| title_short | <i>Bna.EPF2</i> Enhances Drought Tolerance by Regulating Stomatal Development and Stomatal Size in <i>Brassica napus</i> |
| title_sort | i bna epf2 i enhances drought tolerance by regulating stomatal development and stomatal size in i brassica napus i |
| topic | <i>Bna.EPF2</i> <i>Brassica napus</i> drought tolerance stomatal density stomatal dimension water use efficiency |
| url | https://www.mdpi.com/1422-0067/24/9/8007 |
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