Effects of dietary supplementation of bacteriophage cocktail on health status of weanling pigs in a non-sanitary environment
Abstract Background The study evaluated the effects of bacteriophage cocktail (BP) and ZnO administered during weaning time for piglets exposed to a non-sanitary environment. The bacteriophages were designed to eliminate Escherichia coli (K88, K99 and F41), Salmonella (typhimurium and enteritidis),...
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| Format: | Article |
| Language: | English |
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BMC
2023-05-01
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| Series: | Journal of Animal Science and Biotechnology |
| Subjects: | |
| Online Access: | https://doi.org/10.1186/s40104-023-00869-6 |
| _version_ | 1797827384571854848 |
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| author | YoHan Choi Abdolreza Hosseindoust Sang Hun Ha Joeun Kim YeJin Min YongDae Jeong JunYoung Mun SooJin Sa JinSoo Kim |
| author_facet | YoHan Choi Abdolreza Hosseindoust Sang Hun Ha Joeun Kim YeJin Min YongDae Jeong JunYoung Mun SooJin Sa JinSoo Kim |
| author_sort | YoHan Choi |
| collection | DOAJ |
| description | Abstract Background The study evaluated the effects of bacteriophage cocktail (BP) and ZnO administered during weaning time for piglets exposed to a non-sanitary environment. The bacteriophages were designed to eliminate Escherichia coli (K88, K99 and F41), Salmonella (typhimurium and enteritidis), and Clostridium perfreingens (types A and C). Forty 21-day-old crossbreed piglets were assigned to four treatments, including the PC (sanitary environment), NC (non-sanitary environment), BP (NC plus 108 pfu/kg BP), and ZO (NC plus 2,500 mg/kg ZnO). Piglets in the NC, BP and ZO were kept in a non-sanitary environment for 14 d, which was contaminated with the feces of infected pigs. Results Pigs in the BP and ZO treatments had a higher final body weight compared with the NC. The NC treatment showed the highest concentration of inflammatory cytokines including interleukin (IL)-1β, IL-6 and tumor necrosis factor-α in the plasma. The administration of BP and ZO showed lower myeloperoxidase concentrations compared with the NC. The NC treatment showed a lower concentration of superoxide dismutase in serum compared with the PC. Among the treatments in non-sanitary environment, the NC treatment showed a higher concentration of malondialdehyde compared with the ZO. The PC treatment showed a lower concentration of butyric acid in the feces compared with the BP treatment. Among non-sanitary treatments, the villus height in the duodenum was greater in the BP and ZO compared with the NC. The lower abundance of Proteobacteria phylum was observed in the BP and PC treatments compared with the NC. The highest relative abundance of Eubacterium was recorded in the BP treatment. The abundance of Megasphaera and Schwartzia was higher in the NC pigs compared with the BP piglets. The abundance of Desulfovibrio was lower in the supplemented treatments (BP and ZO) compared with non-supplemented (NC and PC). The abundance of Cellulosilyticum genera was higher in the BP and ZO treatments rather than in the NC. The piglets in the NC treatment had the highest abundance of Escherichia-Shigella, followed by the PC and ZO treatments. Conclusion In conclusion, these results suggest that the supplementation of bacteriophage cocktail could effectively control Proteobacteria phylum, Clostridium spp. and coliforms population and mitigated the adverse influences of weaning stress in piglets. |
| first_indexed | 2024-04-09T12:47:25Z |
| format | Article |
| id | doaj.art-81e4b288cd824b99934b7435dd03d52f |
| institution | Directory Open Access Journal |
| issn | 2049-1891 |
| language | English |
| last_indexed | 2024-04-09T12:47:25Z |
| publishDate | 2023-05-01 |
| publisher | BMC |
| record_format | Article |
| series | Journal of Animal Science and Biotechnology |
| spelling | doaj.art-81e4b288cd824b99934b7435dd03d52f2023-05-14T11:23:48ZengBMCJournal of Animal Science and Biotechnology2049-18912023-05-0114111710.1186/s40104-023-00869-6Effects of dietary supplementation of bacteriophage cocktail on health status of weanling pigs in a non-sanitary environmentYoHan Choi0Abdolreza Hosseindoust1Sang Hun Ha2Joeun Kim3YeJin Min4YongDae Jeong5JunYoung Mun6SooJin Sa7JinSoo Kim8Swine Science Division, National Institute of Animal Science, Rural Development AdministrationDepartment of Animal Industry Convergence, Kangwon National UniversityDepartment of Animal Industry Convergence, Kangwon National UniversitySwine Science Division, National Institute of Animal Science, Rural Development AdministrationSwine Science Division, National Institute of Animal Science, Rural Development AdministrationSwine Science Division, National Institute of Animal Science, Rural Development AdministrationDepartment of Animal Industry Convergence, Kangwon National UniversitySwine Science Division, National Institute of Animal Science, Rural Development AdministrationDepartment of Animal Industry Convergence, Kangwon National UniversityAbstract Background The study evaluated the effects of bacteriophage cocktail (BP) and ZnO administered during weaning time for piglets exposed to a non-sanitary environment. The bacteriophages were designed to eliminate Escherichia coli (K88, K99 and F41), Salmonella (typhimurium and enteritidis), and Clostridium perfreingens (types A and C). Forty 21-day-old crossbreed piglets were assigned to four treatments, including the PC (sanitary environment), NC (non-sanitary environment), BP (NC plus 108 pfu/kg BP), and ZO (NC plus 2,500 mg/kg ZnO). Piglets in the NC, BP and ZO were kept in a non-sanitary environment for 14 d, which was contaminated with the feces of infected pigs. Results Pigs in the BP and ZO treatments had a higher final body weight compared with the NC. The NC treatment showed the highest concentration of inflammatory cytokines including interleukin (IL)-1β, IL-6 and tumor necrosis factor-α in the plasma. The administration of BP and ZO showed lower myeloperoxidase concentrations compared with the NC. The NC treatment showed a lower concentration of superoxide dismutase in serum compared with the PC. Among the treatments in non-sanitary environment, the NC treatment showed a higher concentration of malondialdehyde compared with the ZO. The PC treatment showed a lower concentration of butyric acid in the feces compared with the BP treatment. Among non-sanitary treatments, the villus height in the duodenum was greater in the BP and ZO compared with the NC. The lower abundance of Proteobacteria phylum was observed in the BP and PC treatments compared with the NC. The highest relative abundance of Eubacterium was recorded in the BP treatment. The abundance of Megasphaera and Schwartzia was higher in the NC pigs compared with the BP piglets. The abundance of Desulfovibrio was lower in the supplemented treatments (BP and ZO) compared with non-supplemented (NC and PC). The abundance of Cellulosilyticum genera was higher in the BP and ZO treatments rather than in the NC. The piglets in the NC treatment had the highest abundance of Escherichia-Shigella, followed by the PC and ZO treatments. Conclusion In conclusion, these results suggest that the supplementation of bacteriophage cocktail could effectively control Proteobacteria phylum, Clostridium spp. and coliforms population and mitigated the adverse influences of weaning stress in piglets.https://doi.org/10.1186/s40104-023-00869-6AntioxidantCytokinesDiarrheaFecal scoreInflammationMicrobiota |
| spellingShingle | YoHan Choi Abdolreza Hosseindoust Sang Hun Ha Joeun Kim YeJin Min YongDae Jeong JunYoung Mun SooJin Sa JinSoo Kim Effects of dietary supplementation of bacteriophage cocktail on health status of weanling pigs in a non-sanitary environment Journal of Animal Science and Biotechnology Antioxidant Cytokines Diarrhea Fecal score Inflammation Microbiota |
| title | Effects of dietary supplementation of bacteriophage cocktail on health status of weanling pigs in a non-sanitary environment |
| title_full | Effects of dietary supplementation of bacteriophage cocktail on health status of weanling pigs in a non-sanitary environment |
| title_fullStr | Effects of dietary supplementation of bacteriophage cocktail on health status of weanling pigs in a non-sanitary environment |
| title_full_unstemmed | Effects of dietary supplementation of bacteriophage cocktail on health status of weanling pigs in a non-sanitary environment |
| title_short | Effects of dietary supplementation of bacteriophage cocktail on health status of weanling pigs in a non-sanitary environment |
| title_sort | effects of dietary supplementation of bacteriophage cocktail on health status of weanling pigs in a non sanitary environment |
| topic | Antioxidant Cytokines Diarrhea Fecal score Inflammation Microbiota |
| url | https://doi.org/10.1186/s40104-023-00869-6 |
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