Pathogenicity of Isolates of Serratia Marcescens towards Larvae of the Scarab Phyllophaga Blanchardi (Coleoptera)
Serratia marcescens is a Gram negative bacterium (Enterobacteriaceae) often associated with infection of insects. In order to find pathogenic bacteria with the potential to control scarab larvae, several bacterial strains were isolated from the hemocoel of diseased Phyllophaga spp (Coleoptera:Scarab...
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MDPI AG
2015-05-01
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| Series: | Pathogens |
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| Online Access: | http://www.mdpi.com/2076-0817/4/2/210 |
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| author | Mónica L. Pineda-Castellanos Zitlhally Rodríguez-Segura Francisco J. Villalobos Luciano Hernández Laura Lina M. Eugenia Nuñez-Valdez |
| author_facet | Mónica L. Pineda-Castellanos Zitlhally Rodríguez-Segura Francisco J. Villalobos Luciano Hernández Laura Lina M. Eugenia Nuñez-Valdez |
| author_sort | Mónica L. Pineda-Castellanos |
| collection | DOAJ |
| description | Serratia marcescens is a Gram negative bacterium (Enterobacteriaceae) often associated with infection of insects. In order to find pathogenic bacteria with the potential to control scarab larvae, several bacterial strains were isolated from the hemocoel of diseased Phyllophaga spp (Coleoptera:Scarabaeidae) larvae collected from cornfields in Mexico. Five isolates were identified as Serratia marcescens by 16S rRNA gene sequencing and biochemical tests. Oral and injection bioassays using healthy Phyllophaga blanchardi larvae fed with the S. marcescens isolates showed different degrees of antifeeding effect and mortality. No insecticidal activity was observed for Spodoptera frugiperda larvae (Lepidoptera: Noctuidae) by oral inoculation. S. marcescens (Sm81) cell-free culture supernatant caused significant antifeeding effect and mortality to P. blanchardi larvae by oral bioassay and also mortality by injection bioassay. Heat treated culture broths lost the ability to cause disease symptoms, suggesting the involvement of proteins in the toxic activity. A protein of 50.2 kDa was purified from the cell-free broth and showed insecticidal activity by injection bioassay towards P. blanchardi. Analysis of the insecticidal protein by tandem- mass spectrometry (LC-MS/MS) showed similarity to a Serralysin-like protein from S. marcescens spp. This insecticidal protein could have applications in agricultural biotechnology. |
| first_indexed | 2024-04-11T12:39:51Z |
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| id | doaj.art-81e61ff5f34e428eb3f2bcfc124b1b3d |
| institution | Directory Open Access Journal |
| issn | 2076-0817 |
| language | English |
| last_indexed | 2024-04-11T12:39:51Z |
| publishDate | 2015-05-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Pathogens |
| spelling | doaj.art-81e61ff5f34e428eb3f2bcfc124b1b3d2022-12-22T04:23:32ZengMDPI AGPathogens2076-08172015-05-014221022810.3390/pathogens4020210pathogens4020210Pathogenicity of Isolates of Serratia Marcescens towards Larvae of the Scarab Phyllophaga Blanchardi (Coleoptera)Mónica L. Pineda-Castellanos0Zitlhally Rodríguez-Segura1Francisco J. Villalobos2Luciano Hernández3Laura Lina4M. Eugenia Nuñez-Valdez5Centro de Investigación en Dinámica Celular, Instituto de Ciencias Básicas y Aplicadas, Universidad Autónoma del Estado de Morelos, Av. Universidad 1001, Col. Chamilpa, CP 62209, Cuernavaca, Morelos, MexicoCentro de Investigación en Biotecnología, Universidad Autónoma del Estado de Morelos, Av. Universidad 1001, Col. Chamilpa, 62209, Cuernavaca, Morelos, MexicoEl Colegio de la Frontera Sur, Carretera Panamericana y Periférico Sur s/n Barrio María Auxiliadora, CP 29290, San Cristóbal de Las Casas, Chiapas, MexicoFacultad de Química, Universidad Nacional Autónoma de Mexico, Mexico D.F., MexicoCentro de Investigación en Biotecnología, Universidad Autónoma del Estado de Morelos, Av. Universidad 1001, Col. Chamilpa, 62209, Cuernavaca, Morelos, MexicoCentro de Investigación en Dinámica Celular, Instituto de Ciencias Básicas y Aplicadas, Universidad Autónoma del Estado de Morelos, Av. Universidad 1001, Col. Chamilpa, CP 62209, Cuernavaca, Morelos, MexicoSerratia marcescens is a Gram negative bacterium (Enterobacteriaceae) often associated with infection of insects. In order to find pathogenic bacteria with the potential to control scarab larvae, several bacterial strains were isolated from the hemocoel of diseased Phyllophaga spp (Coleoptera:Scarabaeidae) larvae collected from cornfields in Mexico. Five isolates were identified as Serratia marcescens by 16S rRNA gene sequencing and biochemical tests. Oral and injection bioassays using healthy Phyllophaga blanchardi larvae fed with the S. marcescens isolates showed different degrees of antifeeding effect and mortality. No insecticidal activity was observed for Spodoptera frugiperda larvae (Lepidoptera: Noctuidae) by oral inoculation. S. marcescens (Sm81) cell-free culture supernatant caused significant antifeeding effect and mortality to P. blanchardi larvae by oral bioassay and also mortality by injection bioassay. Heat treated culture broths lost the ability to cause disease symptoms, suggesting the involvement of proteins in the toxic activity. A protein of 50.2 kDa was purified from the cell-free broth and showed insecticidal activity by injection bioassay towards P. blanchardi. Analysis of the insecticidal protein by tandem- mass spectrometry (LC-MS/MS) showed similarity to a Serralysin-like protein from S. marcescens spp. This insecticidal protein could have applications in agricultural biotechnology.http://www.mdpi.com/2076-0817/4/2/210Serratia marcescenswhite grubpathogenicityPhyllophaga scarabbiological controlinsecticidal proteinserralysin |
| spellingShingle | Mónica L. Pineda-Castellanos Zitlhally Rodríguez-Segura Francisco J. Villalobos Luciano Hernández Laura Lina M. Eugenia Nuñez-Valdez Pathogenicity of Isolates of Serratia Marcescens towards Larvae of the Scarab Phyllophaga Blanchardi (Coleoptera) Pathogens Serratia marcescens white grub pathogenicity Phyllophaga scarab biological control insecticidal protein serralysin |
| title | Pathogenicity of Isolates of Serratia Marcescens towards Larvae of the Scarab Phyllophaga Blanchardi (Coleoptera) |
| title_full | Pathogenicity of Isolates of Serratia Marcescens towards Larvae of the Scarab Phyllophaga Blanchardi (Coleoptera) |
| title_fullStr | Pathogenicity of Isolates of Serratia Marcescens towards Larvae of the Scarab Phyllophaga Blanchardi (Coleoptera) |
| title_full_unstemmed | Pathogenicity of Isolates of Serratia Marcescens towards Larvae of the Scarab Phyllophaga Blanchardi (Coleoptera) |
| title_short | Pathogenicity of Isolates of Serratia Marcescens towards Larvae of the Scarab Phyllophaga Blanchardi (Coleoptera) |
| title_sort | pathogenicity of isolates of serratia marcescens towards larvae of the scarab phyllophaga blanchardi coleoptera |
| topic | Serratia marcescens white grub pathogenicity Phyllophaga scarab biological control insecticidal protein serralysin |
| url | http://www.mdpi.com/2076-0817/4/2/210 |
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