Effect of stem cell conditional medium-loading adhesive hydrogel on TGF-β1-induced endometrial stromal cell fibrosis
Introduction: Uterine adhesion (IUA) is a severe complication that results from uterine operations or uterine infections. Hysteroscopy is considered the gold standard for the diagnosis and treatment of uterine adhesions. Yet, this invasive procedure leads to re-adhesions after hysteroscopic treatmen...
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| Format: | Article |
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Frontiers Media S.A.
2023-05-01
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| Series: | Frontiers in Bioengineering and Biotechnology |
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| Online Access: | https://www.frontiersin.org/articles/10.3389/fbioe.2023.1168136/full |
| _version_ | 1797833576595587072 |
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| author | Yuan Zhu Yuan Zhu Ting Wang Ming-Jie Bao Xiao-Hui Qu Zeng-Ming Li |
| author_facet | Yuan Zhu Yuan Zhu Ting Wang Ming-Jie Bao Xiao-Hui Qu Zeng-Ming Li |
| author_sort | Yuan Zhu |
| collection | DOAJ |
| description | Introduction: Uterine adhesion (IUA) is a severe complication that results from uterine operations or uterine infections. Hysteroscopy is considered the gold standard for the diagnosis and treatment of uterine adhesions. Yet, this invasive procedure leads to re-adhesions after hysteroscopic treatment. Hydrogels loading functional additives (e.g., placental mesenchymal stem cells (PC-MSCs)) that can act as physical barriers and promote endometrium regeneration are a good solution. However, traditional hydrogels lack tissue adhesion which makes them unstable under a rapid turnover of the uterus, and PC-MSCs have biosafety risks when used as functional additives.Methods: In this study, we coupled an adhesive hydrogel with a PC-MSCs conditioned medium (CM) to form a hybrid of gel and functional additives (CM/Gel-MA).Results and Discussion: Our experiments show that CM/Gel-MA enhances the activity of endometrial stromal cells (ESCs), promotes cell proliferation, and reduces the expression of α-SMA, collagen I, CTGF, E-cadherin, and IL-6, which helps to reduce the inflammatory response and inhibit fibrosis. We conclude that CM/Gel-MA can more potentially prevent IUA by combining the physical barriers from adhesive hydrogel and functional promotion from CM. |
| first_indexed | 2024-04-09T14:26:10Z |
| format | Article |
| id | doaj.art-81e7aa79f0084fbb805febab9ef6b399 |
| institution | Directory Open Access Journal |
| issn | 2296-4185 |
| language | English |
| last_indexed | 2024-04-09T14:26:10Z |
| publishDate | 2023-05-01 |
| publisher | Frontiers Media S.A. |
| record_format | Article |
| series | Frontiers in Bioengineering and Biotechnology |
| spelling | doaj.art-81e7aa79f0084fbb805febab9ef6b3992023-05-04T04:34:33ZengFrontiers Media S.A.Frontiers in Bioengineering and Biotechnology2296-41852023-05-011110.3389/fbioe.2023.11681361168136Effect of stem cell conditional medium-loading adhesive hydrogel on TGF-β1-induced endometrial stromal cell fibrosisYuan Zhu0Yuan Zhu1Ting Wang2Ming-Jie Bao3Xiao-Hui Qu4Zeng-Ming Li5JXHC Key Laboratory of Fertility Preservation, Jiangxi Provincial Maternal and Child Health Hospital, Nanchang, Jiangxi, ChinaDepartment of Reproductive Health, Jiangxi Provincial Maternal and Child Health Hospital, Nanchang, Jiangxi, ChinaDepartment of Reproductive Health, Jiangxi Provincial Maternal and Child Health Hospital, Nanchang, Jiangxi, ChinaDepartment of Reproductive Health, Jiangxi Provincial Maternal and Child Health Hospital, Nanchang, Jiangxi, ChinaPathology Department, Jiangxi Provincial Maternal and Child Health Hospital, Nanchang, Jiangxi, ChinaJXHC Key Laboratory of Fertility Preservation, Jiangxi Provincial Maternal and Child Health Hospital, Nanchang, Jiangxi, ChinaIntroduction: Uterine adhesion (IUA) is a severe complication that results from uterine operations or uterine infections. Hysteroscopy is considered the gold standard for the diagnosis and treatment of uterine adhesions. Yet, this invasive procedure leads to re-adhesions after hysteroscopic treatment. Hydrogels loading functional additives (e.g., placental mesenchymal stem cells (PC-MSCs)) that can act as physical barriers and promote endometrium regeneration are a good solution. However, traditional hydrogels lack tissue adhesion which makes them unstable under a rapid turnover of the uterus, and PC-MSCs have biosafety risks when used as functional additives.Methods: In this study, we coupled an adhesive hydrogel with a PC-MSCs conditioned medium (CM) to form a hybrid of gel and functional additives (CM/Gel-MA).Results and Discussion: Our experiments show that CM/Gel-MA enhances the activity of endometrial stromal cells (ESCs), promotes cell proliferation, and reduces the expression of α-SMA, collagen I, CTGF, E-cadherin, and IL-6, which helps to reduce the inflammatory response and inhibit fibrosis. We conclude that CM/Gel-MA can more potentially prevent IUA by combining the physical barriers from adhesive hydrogel and functional promotion from CM.https://www.frontiersin.org/articles/10.3389/fbioe.2023.1168136/fulladhesive hydrogelstem cell conditional mediumendometrial stromal celluterine adhesionfibrosis |
| spellingShingle | Yuan Zhu Yuan Zhu Ting Wang Ming-Jie Bao Xiao-Hui Qu Zeng-Ming Li Effect of stem cell conditional medium-loading adhesive hydrogel on TGF-β1-induced endometrial stromal cell fibrosis Frontiers in Bioengineering and Biotechnology adhesive hydrogel stem cell conditional medium endometrial stromal cell uterine adhesion fibrosis |
| title | Effect of stem cell conditional medium-loading adhesive hydrogel on TGF-β1-induced endometrial stromal cell fibrosis |
| title_full | Effect of stem cell conditional medium-loading adhesive hydrogel on TGF-β1-induced endometrial stromal cell fibrosis |
| title_fullStr | Effect of stem cell conditional medium-loading adhesive hydrogel on TGF-β1-induced endometrial stromal cell fibrosis |
| title_full_unstemmed | Effect of stem cell conditional medium-loading adhesive hydrogel on TGF-β1-induced endometrial stromal cell fibrosis |
| title_short | Effect of stem cell conditional medium-loading adhesive hydrogel on TGF-β1-induced endometrial stromal cell fibrosis |
| title_sort | effect of stem cell conditional medium loading adhesive hydrogel on tgf β1 induced endometrial stromal cell fibrosis |
| topic | adhesive hydrogel stem cell conditional medium endometrial stromal cell uterine adhesion fibrosis |
| url | https://www.frontiersin.org/articles/10.3389/fbioe.2023.1168136/full |
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