Smoking status and anti-inflammatory macrophages in bronchoalveolar lavage and induced sputum in COPD
<p>Abstract</p> <p>Background</p> <p>Macrophages have been implicated in the pathogenesis of COPD. M1 and M2 macrophages constitute subpopulations displaying pro- and anti-inflammatory properties. We hypothesized that smoking cessation affects macrophage heterogeneity i...
| Main Authors: | , , , , , , , , , , |
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| Format: | Article |
| Language: | English |
| Published: |
BMC
2011-03-01
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| Series: | Respiratory Research |
| Online Access: | http://respiratory-research.com/content/12/1/34 |
| _version_ | 1818682285838303232 |
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| author | Rabe Klaus F Schrumpf Jasmijn A van Wijngaarden Simone Timens Wim Budulac Simona E Snoeck-Stroband Jiska B Lapperre Thérèse S Kunz Lisette IZ Postma Dirkje S Sterk Peter J Hiemstra Pieter S |
| author_facet | Rabe Klaus F Schrumpf Jasmijn A van Wijngaarden Simone Timens Wim Budulac Simona E Snoeck-Stroband Jiska B Lapperre Thérèse S Kunz Lisette IZ Postma Dirkje S Sterk Peter J Hiemstra Pieter S |
| author_sort | Rabe Klaus F |
| collection | DOAJ |
| description | <p>Abstract</p> <p>Background</p> <p>Macrophages have been implicated in the pathogenesis of COPD. M1 and M2 macrophages constitute subpopulations displaying pro- and anti-inflammatory properties. We hypothesized that smoking cessation affects macrophage heterogeneity in the lung of patients with COPD. Our aim was to study macrophage heterogeneity using the M2-marker CD163 and selected pro- and anti-inflammatory mediators in bronchoalveolar lavage (BAL) fluid and induced sputum from current smokers and ex-smokers with COPD.</p> <p>Methods</p> <p>114 COPD patients (72 current smokers; 42 ex-smokers, median smoking cessation 3.5 years) were studied cross-sectionally and underwent sputum induction (M/F 99/15, age 62 ± 8 [mean ± SD] years, 42 (31-55) [median (range)] packyears, post-bronchodilator FEV<sub>1 </sub>63 ± 9% predicted, no steroids past 6 months). BAL was collected from 71 patients. CD163<sup>+ </sup>macrophages were quantified in BAL and sputum cytospins. Pro- and anti-inflammatory mediators were measured in BAL and sputum supernatants.</p> <p>Results</p> <p>Ex-smokers with COPD had a higher percentage, but lower number of CD163<sup>+ </sup>macrophages in BAL than current smokers (83.5% and 68.0%, p = 0.04; 5.6 and 20.1 ×10<sup>4</sup>/ml, p = 0.001 respectively). The percentage CD163<sup>+ </sup>M2 macrophages was higher in BAL compared to sputum (74.0% and 30.3%, p < 0.001). BAL M-CSF levels were higher in smokers than ex-smokers (571 pg/ml and 150 pg/ml, p = 0.001) and correlated with the number of CD163<sup>+ </sup>BAL macrophages (Rs = 0.38, p = 0.003). No significant differences were found between smokers and ex-smokers in the levels of pro-inflammatory (IL-6 and IL-8), and anti-inflammatory (elafin, and Secretory Leukocyte Protease Inhibitor [SLPI]) mediators in BAL and sputum.</p> <p>Conclusions</p> <p>Our data suggest that smoking cessation partially changes the macrophage polarization <it>in vivo </it>in the periphery of the lung towards an anti-inflammatory phenotype, which is not accompanied by a decrease in inflammatory parameters.</p> |
| first_indexed | 2024-12-17T10:16:25Z |
| format | Article |
| id | doaj.art-81e8dd710c7548e1b167242db51ac19f |
| institution | Directory Open Access Journal |
| issn | 1465-9921 |
| language | English |
| last_indexed | 2024-12-17T10:16:25Z |
| publishDate | 2011-03-01 |
| publisher | BMC |
| record_format | Article |
| series | Respiratory Research |
| spelling | doaj.art-81e8dd710c7548e1b167242db51ac19f2022-12-21T21:52:54ZengBMCRespiratory Research1465-99212011-03-011213410.1186/1465-9921-12-34Smoking status and anti-inflammatory macrophages in bronchoalveolar lavage and induced sputum in COPDRabe Klaus FSchrumpf Jasmijn Avan Wijngaarden SimoneTimens WimBudulac Simona ESnoeck-Stroband Jiska BLapperre Thérèse SKunz Lisette IZPostma Dirkje SSterk Peter JHiemstra Pieter S<p>Abstract</p> <p>Background</p> <p>Macrophages have been implicated in the pathogenesis of COPD. M1 and M2 macrophages constitute subpopulations displaying pro- and anti-inflammatory properties. We hypothesized that smoking cessation affects macrophage heterogeneity in the lung of patients with COPD. Our aim was to study macrophage heterogeneity using the M2-marker CD163 and selected pro- and anti-inflammatory mediators in bronchoalveolar lavage (BAL) fluid and induced sputum from current smokers and ex-smokers with COPD.</p> <p>Methods</p> <p>114 COPD patients (72 current smokers; 42 ex-smokers, median smoking cessation 3.5 years) were studied cross-sectionally and underwent sputum induction (M/F 99/15, age 62 ± 8 [mean ± SD] years, 42 (31-55) [median (range)] packyears, post-bronchodilator FEV<sub>1 </sub>63 ± 9% predicted, no steroids past 6 months). BAL was collected from 71 patients. CD163<sup>+ </sup>macrophages were quantified in BAL and sputum cytospins. Pro- and anti-inflammatory mediators were measured in BAL and sputum supernatants.</p> <p>Results</p> <p>Ex-smokers with COPD had a higher percentage, but lower number of CD163<sup>+ </sup>macrophages in BAL than current smokers (83.5% and 68.0%, p = 0.04; 5.6 and 20.1 ×10<sup>4</sup>/ml, p = 0.001 respectively). The percentage CD163<sup>+ </sup>M2 macrophages was higher in BAL compared to sputum (74.0% and 30.3%, p < 0.001). BAL M-CSF levels were higher in smokers than ex-smokers (571 pg/ml and 150 pg/ml, p = 0.001) and correlated with the number of CD163<sup>+ </sup>BAL macrophages (Rs = 0.38, p = 0.003). No significant differences were found between smokers and ex-smokers in the levels of pro-inflammatory (IL-6 and IL-8), and anti-inflammatory (elafin, and Secretory Leukocyte Protease Inhibitor [SLPI]) mediators in BAL and sputum.</p> <p>Conclusions</p> <p>Our data suggest that smoking cessation partially changes the macrophage polarization <it>in vivo </it>in the periphery of the lung towards an anti-inflammatory phenotype, which is not accompanied by a decrease in inflammatory parameters.</p>http://respiratory-research.com/content/12/1/34 |
| spellingShingle | Rabe Klaus F Schrumpf Jasmijn A van Wijngaarden Simone Timens Wim Budulac Simona E Snoeck-Stroband Jiska B Lapperre Thérèse S Kunz Lisette IZ Postma Dirkje S Sterk Peter J Hiemstra Pieter S Smoking status and anti-inflammatory macrophages in bronchoalveolar lavage and induced sputum in COPD Respiratory Research |
| title | Smoking status and anti-inflammatory macrophages in bronchoalveolar lavage and induced sputum in COPD |
| title_full | Smoking status and anti-inflammatory macrophages in bronchoalveolar lavage and induced sputum in COPD |
| title_fullStr | Smoking status and anti-inflammatory macrophages in bronchoalveolar lavage and induced sputum in COPD |
| title_full_unstemmed | Smoking status and anti-inflammatory macrophages in bronchoalveolar lavage and induced sputum in COPD |
| title_short | Smoking status and anti-inflammatory macrophages in bronchoalveolar lavage and induced sputum in COPD |
| title_sort | smoking status and anti inflammatory macrophages in bronchoalveolar lavage and induced sputum in copd |
| url | http://respiratory-research.com/content/12/1/34 |
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