Preparation of a pH‐responsive chitosan‐montmorillonite‐nitrogen‐doped carbon quantum dots nanocarrier for attenuating doxorubicin limitations in cancer therapy
Abstract Despite its widespread usage as a chemotherapy drug in cancer treatment, doxorubicin (DOX) has limitations such as short in vivo circulation time, low solubility, and poor permeability. In this regard, a pH‐responsive chitosan (CS)‐ montmorillonite (MMT)‐ nitrogen‐doped carbon quantum dots...
| Main Authors: | , , , , , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
Wiley-VCH
2022-10-01
|
| Series: | Engineering in Life Sciences |
| Subjects: | |
| Online Access: | https://doi.org/10.1002/elsc.202200016 |
| _version_ | 1811195145141354496 |
|---|---|
| author | Erfan Rahmani Mehrab Pourmadadi Sohrab Ali Ghorbanian Fatemeh Yazdian Hamid Rashedi Mona Navaee |
| author_facet | Erfan Rahmani Mehrab Pourmadadi Sohrab Ali Ghorbanian Fatemeh Yazdian Hamid Rashedi Mona Navaee |
| author_sort | Erfan Rahmani |
| collection | DOAJ |
| description | Abstract Despite its widespread usage as a chemotherapy drug in cancer treatment, doxorubicin (DOX) has limitations such as short in vivo circulation time, low solubility, and poor permeability. In this regard, a pH‐responsive chitosan (CS)‐ montmorillonite (MMT)‐ nitrogen‐doped carbon quantum dots (NCQDs) nanocomposite was first developed, loaded with DOX, and then incorporated into a double emulsion to further develop the sustained release. The incorporated NCQDs into the CS‐MMT hydrogel exhibited enhanced loading and entrapment efficiencies. The presence of NCQDs nanoparticles in the CS‐MMT hydrogel also resulted in an extended pH‐responsive release of DOX over a period of 96 h compared to that of CS‐MMT‐DOX nanocarriers at pH 5.4. Based on the Korsmeyer‐Peppas model, there was a controlled DOX release at pH 5.4, while no diffusion was observed at pH 7.4, indicating fewer side effects. MTT assay showed that the cytotoxicity of DOX‐loaded CS‐MMT‐NCQDs hydrogel nanocomposite was significantly higher than those of free DOX (p < 0.001) and CS‐MMT‐NCQDs (p < 0.001) on MCF‐7 cells. Flow cytometry results demonstrated that a higher apoptosis induction achieved after incorporating NCQDs nanoparticles into CS‐MMT‐DOX nanocarrier. These findings suggest that the DOX‐loaded nanocomposite is a promising candidate for the targeted treatment of cancer cells. |
| first_indexed | 2024-04-12T00:38:33Z |
| format | Article |
| id | doaj.art-81e9624918b04bd8831bda783e1bf70c |
| institution | Directory Open Access Journal |
| issn | 1618-0240 1618-2863 |
| language | English |
| last_indexed | 2024-04-12T00:38:33Z |
| publishDate | 2022-10-01 |
| publisher | Wiley-VCH |
| record_format | Article |
| series | Engineering in Life Sciences |
| spelling | doaj.art-81e9624918b04bd8831bda783e1bf70c2022-12-22T03:55:05ZengWiley-VCHEngineering in Life Sciences1618-02401618-28632022-10-01221063464910.1002/elsc.202200016Preparation of a pH‐responsive chitosan‐montmorillonite‐nitrogen‐doped carbon quantum dots nanocarrier for attenuating doxorubicin limitations in cancer therapyErfan Rahmani0Mehrab Pourmadadi1Sohrab Ali Ghorbanian2Fatemeh Yazdian3Hamid Rashedi4Mona Navaee5School of Chemical Engineering College of Engineering University of Tehran Tehran IranSchool of Chemical Engineering College of Engineering University of Tehran Tehran IranSchool of Chemical Engineering College of Engineering University of Tehran Tehran IranDepartment of Life Science Engineering Faculty of New Science and Technologies University of Tehran Tehran IranDepartment of Biotechnology School of Chemical Engineering College of Engineering University of Tehran Tehran IranPharmaceutical Sciences Research Center The Institute of Pharmaceutical Sciences (TIPS) Tehran University of Medical Sciences (TUMS) Tehran IranAbstract Despite its widespread usage as a chemotherapy drug in cancer treatment, doxorubicin (DOX) has limitations such as short in vivo circulation time, low solubility, and poor permeability. In this regard, a pH‐responsive chitosan (CS)‐ montmorillonite (MMT)‐ nitrogen‐doped carbon quantum dots (NCQDs) nanocomposite was first developed, loaded with DOX, and then incorporated into a double emulsion to further develop the sustained release. The incorporated NCQDs into the CS‐MMT hydrogel exhibited enhanced loading and entrapment efficiencies. The presence of NCQDs nanoparticles in the CS‐MMT hydrogel also resulted in an extended pH‐responsive release of DOX over a period of 96 h compared to that of CS‐MMT‐DOX nanocarriers at pH 5.4. Based on the Korsmeyer‐Peppas model, there was a controlled DOX release at pH 5.4, while no diffusion was observed at pH 7.4, indicating fewer side effects. MTT assay showed that the cytotoxicity of DOX‐loaded CS‐MMT‐NCQDs hydrogel nanocomposite was significantly higher than those of free DOX (p < 0.001) and CS‐MMT‐NCQDs (p < 0.001) on MCF‐7 cells. Flow cytometry results demonstrated that a higher apoptosis induction achieved after incorporating NCQDs nanoparticles into CS‐MMT‐DOX nanocarrier. These findings suggest that the DOX‐loaded nanocomposite is a promising candidate for the targeted treatment of cancer cells.https://doi.org/10.1002/elsc.202200016carbon quantum dotschitosandoxorubicindrug deliverymontmorillonitepH‐responsive nanocarrier |
| spellingShingle | Erfan Rahmani Mehrab Pourmadadi Sohrab Ali Ghorbanian Fatemeh Yazdian Hamid Rashedi Mona Navaee Preparation of a pH‐responsive chitosan‐montmorillonite‐nitrogen‐doped carbon quantum dots nanocarrier for attenuating doxorubicin limitations in cancer therapy Engineering in Life Sciences carbon quantum dots chitosan doxorubicin drug delivery montmorillonite pH‐responsive nanocarrier |
| title | Preparation of a pH‐responsive chitosan‐montmorillonite‐nitrogen‐doped carbon quantum dots nanocarrier for attenuating doxorubicin limitations in cancer therapy |
| title_full | Preparation of a pH‐responsive chitosan‐montmorillonite‐nitrogen‐doped carbon quantum dots nanocarrier for attenuating doxorubicin limitations in cancer therapy |
| title_fullStr | Preparation of a pH‐responsive chitosan‐montmorillonite‐nitrogen‐doped carbon quantum dots nanocarrier for attenuating doxorubicin limitations in cancer therapy |
| title_full_unstemmed | Preparation of a pH‐responsive chitosan‐montmorillonite‐nitrogen‐doped carbon quantum dots nanocarrier for attenuating doxorubicin limitations in cancer therapy |
| title_short | Preparation of a pH‐responsive chitosan‐montmorillonite‐nitrogen‐doped carbon quantum dots nanocarrier for attenuating doxorubicin limitations in cancer therapy |
| title_sort | preparation of a ph responsive chitosan montmorillonite nitrogen doped carbon quantum dots nanocarrier for attenuating doxorubicin limitations in cancer therapy |
| topic | carbon quantum dots chitosan doxorubicin drug delivery montmorillonite pH‐responsive nanocarrier |
| url | https://doi.org/10.1002/elsc.202200016 |
| work_keys_str_mv | AT erfanrahmani preparationofaphresponsivechitosanmontmorillonitenitrogendopedcarbonquantumdotsnanocarrierforattenuatingdoxorubicinlimitationsincancertherapy AT mehrabpourmadadi preparationofaphresponsivechitosanmontmorillonitenitrogendopedcarbonquantumdotsnanocarrierforattenuatingdoxorubicinlimitationsincancertherapy AT sohrabalighorbanian preparationofaphresponsivechitosanmontmorillonitenitrogendopedcarbonquantumdotsnanocarrierforattenuatingdoxorubicinlimitationsincancertherapy AT fatemehyazdian preparationofaphresponsivechitosanmontmorillonitenitrogendopedcarbonquantumdotsnanocarrierforattenuatingdoxorubicinlimitationsincancertherapy AT hamidrashedi preparationofaphresponsivechitosanmontmorillonitenitrogendopedcarbonquantumdotsnanocarrierforattenuatingdoxorubicinlimitationsincancertherapy AT monanavaee preparationofaphresponsivechitosanmontmorillonitenitrogendopedcarbonquantumdotsnanocarrierforattenuatingdoxorubicinlimitationsincancertherapy |