Inhibition of Obtusifolin on retinal pigment epithelial cell growth under hypoxia
AIM: To explore the effect of Obtusifolin on retinal pigment epithelial cell growth under hypoxia. METHODS: In vitro chemical hypoxia model of ARPE-19 cells was established using cobalt chloride (CoCl2). Cell viability was tested by cell counting kit-8 (CCK-8) assay. Western blot and real-time quan...
| Main Authors: | , , , , , , , , , , |
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| Format: | Article |
| Language: | English |
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Press of International Journal of Ophthalmology (IJO PRESS)
2019-10-01
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| Series: | International Journal of Ophthalmology |
| Subjects: | |
| Online Access: | http://www.ijo.cn/en_publish/2019/10/20191004.pdf |
| _version_ | 1828369695311396864 |
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| author | Li-Fei Wang Zhong-Yang Yan Ya-Lin Li Yan-Hui Wang Sheng-Juan Zhang Xin Jia Lu Lu Yan-Xia Shang Xin Wang Yun-Huan Li Shan-Yu Li |
| author_facet | Li-Fei Wang Zhong-Yang Yan Ya-Lin Li Yan-Hui Wang Sheng-Juan Zhang Xin Jia Lu Lu Yan-Xia Shang Xin Wang Yun-Huan Li Shan-Yu Li |
| author_sort | Li-Fei Wang |
| collection | DOAJ |
| description | AIM: To explore the effect of Obtusifolin on retinal pigment epithelial cell growth under hypoxia.
METHODS: In vitro chemical hypoxia model of ARPE-19 cells was established using cobalt chloride (CoCl2). Cell viability was tested by cell counting kit-8 (CCK-8) assay. Western blot and real-time quantitative polymerase chain reaction were applied to detect proteins and mRNAs respectively. Flow cytometry was used to examine the cell cycle. Secretion of vascular endothelial growth factor (VEGF) was tested by using enzyme linked immunosorbent assay (ELISA).
RESULTS: Under the chemical hypoxia model established by CoCl2, hypoxia inducible factor-1α (HIF-1α) mRNA and protein levels was up-regulated. Cell viability was increased and the proportion of S phase was higher. Obtusifolin could reduce cell viability under hypoxic conditions and arrest cells in G1 phase. Obtusifolin reduced the expression of Cyclin D1 and proliferating cell nuclear antigen (PCNA) in the hypoxic environment and increased the expression of p53 and p21. The levels of VEGF, VEGFR2 and eNOS proteins and mRNA were significantly increased under hypoxia while Obtusifolin inhibited the increasing.
CONCLUSION: Obtusifolin can inhibit cell growth under hypoxic conditions and down-regulate HIF-1/VEGF/eNOS secretions in ARPE-19 cells. |
| first_indexed | 2024-04-14T06:27:08Z |
| format | Article |
| id | doaj.art-81e9fc2923b14347bcb6eafbe89bc207 |
| institution | Directory Open Access Journal |
| issn | 2222-3959 2227-4898 |
| language | English |
| last_indexed | 2024-04-14T06:27:08Z |
| publishDate | 2019-10-01 |
| publisher | Press of International Journal of Ophthalmology (IJO PRESS) |
| record_format | Article |
| series | International Journal of Ophthalmology |
| spelling | doaj.art-81e9fc2923b14347bcb6eafbe89bc2072022-12-22T02:07:45ZengPress of International Journal of Ophthalmology (IJO PRESS)International Journal of Ophthalmology2222-39592227-48982019-10-0112101539154710.18240/ijo.2019.10.04Inhibition of Obtusifolin on retinal pigment epithelial cell growth under hypoxiaLi-Fei Wang0Zhong-Yang Yan1Ya-Lin Li2Yan-Hui Wang3Sheng-Juan Zhang4Xin Jia5Lu Lu6Yan-Xia Shang7Xin Wang8Yun-Huan Li9Shan-Yu Li10Fundus Surgery Ward, Hebei Provincial Eye Hospital, Xingtai 054001, Hebei Province, ChinaFundus Surgery Ward, Hebei Provincial Eye Hospital, Xingtai 054001, Hebei Province, ChinaFundus Surgery Ward, Hebei Provincial Eye Hospital, Xingtai 054001, Hebei Province, ChinaFundus Surgery Ward, Hebei Provincial Eye Hospital, Xingtai 054001, Hebei Province, ChinaFundus Surgery Ward, Hebei Provincial Eye Hospital, Xingtai 054001, Hebei Province, ChinaFundus Surgery Ward, Hebei Provincial Eye Hospital, Xingtai 054001, Hebei Province, ChinaDiabetic Eye Disease Ward, Hebei Provincial Eye Hospital, Xingtai 054001, Hebei Province, ChinaDiabetic Eye Disease Ward, Hebei Provincial Eye Hospital, Xingtai 054001, Hebei Province, ChinaCorneal Disease Ward, Hebei Provincial Eye Hospital, Xingtai 054001, Hebei Province, ChinaFundus Surgery Ward, Hebei Provincial Eye Hospital, Xingtai 054001, Hebei Province, ChinaFundus Surgery Ward, Hebei Provincial Eye Hospital, Xingtai 054001, Hebei Province, ChinaAIM: To explore the effect of Obtusifolin on retinal pigment epithelial cell growth under hypoxia. METHODS: In vitro chemical hypoxia model of ARPE-19 cells was established using cobalt chloride (CoCl2). Cell viability was tested by cell counting kit-8 (CCK-8) assay. Western blot and real-time quantitative polymerase chain reaction were applied to detect proteins and mRNAs respectively. Flow cytometry was used to examine the cell cycle. Secretion of vascular endothelial growth factor (VEGF) was tested by using enzyme linked immunosorbent assay (ELISA). RESULTS: Under the chemical hypoxia model established by CoCl2, hypoxia inducible factor-1α (HIF-1α) mRNA and protein levels was up-regulated. Cell viability was increased and the proportion of S phase was higher. Obtusifolin could reduce cell viability under hypoxic conditions and arrest cells in G1 phase. Obtusifolin reduced the expression of Cyclin D1 and proliferating cell nuclear antigen (PCNA) in the hypoxic environment and increased the expression of p53 and p21. The levels of VEGF, VEGFR2 and eNOS proteins and mRNA were significantly increased under hypoxia while Obtusifolin inhibited the increasing. CONCLUSION: Obtusifolin can inhibit cell growth under hypoxic conditions and down-regulate HIF-1/VEGF/eNOS secretions in ARPE-19 cells.http://www.ijo.cn/en_publish/2019/10/20191004.pdfretinal pigment epithelial cellsobtusifolinvascular endothelial growth factorhypoxia |
| spellingShingle | Li-Fei Wang Zhong-Yang Yan Ya-Lin Li Yan-Hui Wang Sheng-Juan Zhang Xin Jia Lu Lu Yan-Xia Shang Xin Wang Yun-Huan Li Shan-Yu Li Inhibition of Obtusifolin on retinal pigment epithelial cell growth under hypoxia International Journal of Ophthalmology retinal pigment epithelial cells obtusifolin vascular endothelial growth factor hypoxia |
| title | Inhibition of Obtusifolin on retinal pigment epithelial cell growth under hypoxia |
| title_full | Inhibition of Obtusifolin on retinal pigment epithelial cell growth under hypoxia |
| title_fullStr | Inhibition of Obtusifolin on retinal pigment epithelial cell growth under hypoxia |
| title_full_unstemmed | Inhibition of Obtusifolin on retinal pigment epithelial cell growth under hypoxia |
| title_short | Inhibition of Obtusifolin on retinal pigment epithelial cell growth under hypoxia |
| title_sort | inhibition of obtusifolin on retinal pigment epithelial cell growth under hypoxia |
| topic | retinal pigment epithelial cells obtusifolin vascular endothelial growth factor hypoxia |
| url | http://www.ijo.cn/en_publish/2019/10/20191004.pdf |
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