Antifungal Susceptibility Analysis of Clinical Isolates of Candida parapsilosis in Iran
Background: Candida parapsilosis is an emergent agent of invasive fungal infections. This yeast is one of the five most widespread yeasts concerned in invasive candidiasis. C. parapsilosis stands out as the second most common yeast species isolated from patients with bloodstream infections especiall...
| Main Authors: | , , , , , , , , , |
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| Format: | Article |
| Language: | English |
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Tehran University of Medical Sciences
2016-03-01
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| Series: | Iranian Journal of Public Health |
| Subjects: | |
| Online Access: | https://ijph.tums.ac.ir/index.php/ijph/article/view/6264 |
| _version_ | 1818569848619270144 |
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| author | Ensieh LOTFALI Parivash KORDBACHEH Hossein MIRHENDI Farideh ZAINI Ali GHAJARI Rasoul MOHAMMADI Fatemeh NOORBAKHSH Maryam MOAZENI Aliakbar FALLAHI Sassan REZAIE |
| author_facet | Ensieh LOTFALI Parivash KORDBACHEH Hossein MIRHENDI Farideh ZAINI Ali GHAJARI Rasoul MOHAMMADI Fatemeh NOORBAKHSH Maryam MOAZENI Aliakbar FALLAHI Sassan REZAIE |
| author_sort | Ensieh LOTFALI |
| collection | DOAJ |
| description | Background: Candida parapsilosis is an emergent agent of invasive fungal infections. This yeast is one of the five most widespread yeasts concerned in invasive candidiasis.
C. parapsilosis stands out as the second most common yeast species isolated from patients with bloodstream infections especially in neonates with catheter.
Recently several reports suggested that its reduced susceptibility to azoles and polyene might become a cause for clinical concern, although C. parapsilosis is not believed to be intensely prone to the development of antifungal resistance.
Methods: In the present report, One hundred and twenty clinical isolates of C. parapsilosis complex were identified and differentiated by using PCR-RFLP analysis. The isolates were then analyzed to determine their susceptibility profile to fluconazole (FLU), itraconazole (ITC) and amphotericin B. The minimum inhibitory concentration (MIC) results were analyzed according to the standard CLSI guide.
Results: All of isolates were identified as C. parapsilosis. No C. metapsilosis and C. orthopsilosis strains were found. Evaluation of the antifungal susceptibility profile showed that only three (2.5%) C. parapsilosis were resistant to fluconazole, three (2.5%) C. parapsilosis were resistant to itraconazole and two (1.7%) C. parapsilosis were amphotericin B resistant.
Conclusion: Profiles in clinical isolates of C. parapsilosis can provide important information for the control of antifungal resistance as well as distribution and susceptibility profiles in populations. |
| first_indexed | 2024-12-14T06:53:04Z |
| format | Article |
| id | doaj.art-81eafbd0657544fb82e9f9c08fe7fc2a |
| institution | Directory Open Access Journal |
| issn | 2251-6085 2251-6093 |
| language | English |
| last_indexed | 2024-12-14T06:53:04Z |
| publishDate | 2016-03-01 |
| publisher | Tehran University of Medical Sciences |
| record_format | Article |
| series | Iranian Journal of Public Health |
| spelling | doaj.art-81eafbd0657544fb82e9f9c08fe7fc2a2022-12-21T23:12:50ZengTehran University of Medical SciencesIranian Journal of Public Health2251-60852251-60932016-03-014534854Antifungal Susceptibility Analysis of Clinical Isolates of Candida parapsilosis in IranEnsieh LOTFALI0Parivash KORDBACHEH1Hossein MIRHENDI2Farideh ZAINI3Ali GHAJARI4Rasoul MOHAMMADI5Fatemeh NOORBAKHSH6Maryam MOAZENI7Aliakbar FALLAHI8Sassan REZAIE9Dept. of Medical Mycology and Parasitology, School of Public Health, Tehran University of Medical Sciences, Tehran, IranDept. of Medical Mycology and Parasitology, School of Public Health, Tehran University of Medical Sciences, Tehran, IranDept. of Medical Mycology and Parasitology, School of Public Health, Tehran University of Medical Sciences, Tehran, IranDept. of Medical Mycology and Parasitology, School of Public Health, Tehran University of Medical Sciences, Tehran, IranDept. of Medical Parasitology and Mycology, School of Medicine, Shahid Beheshti University of Medical Sciences, Tehran, IranDept. of Medical, Parasitology and Mycology, School of Medicine, Isfahan University of Medical Sciences, Isfahan, IranDept. of Biology, Faculty of Science, Islamic Azad University, Varamin-Pishva, IranInvasive Fungi Research Center, Dept. of Medical Mycology and Parasitology, School of Medicine, Mazandaran University of Medical Sciences, Sari, IranDept. of Microbiology & Parasitology & Immunology, School of Medicine, Guilan University of Medical Sciences, Rasht, IranDept. of Medical Mycology and Parasitology, School of Public Health, Tehran University of Medical Sciences, Tehran, IranBackground: Candida parapsilosis is an emergent agent of invasive fungal infections. This yeast is one of the five most widespread yeasts concerned in invasive candidiasis. C. parapsilosis stands out as the second most common yeast species isolated from patients with bloodstream infections especially in neonates with catheter. Recently several reports suggested that its reduced susceptibility to azoles and polyene might become a cause for clinical concern, although C. parapsilosis is not believed to be intensely prone to the development of antifungal resistance. Methods: In the present report, One hundred and twenty clinical isolates of C. parapsilosis complex were identified and differentiated by using PCR-RFLP analysis. The isolates were then analyzed to determine their susceptibility profile to fluconazole (FLU), itraconazole (ITC) and amphotericin B. The minimum inhibitory concentration (MIC) results were analyzed according to the standard CLSI guide. Results: All of isolates were identified as C. parapsilosis. No C. metapsilosis and C. orthopsilosis strains were found. Evaluation of the antifungal susceptibility profile showed that only three (2.5%) C. parapsilosis were resistant to fluconazole, three (2.5%) C. parapsilosis were resistant to itraconazole and two (1.7%) C. parapsilosis were amphotericin B resistant. Conclusion: Profiles in clinical isolates of C. parapsilosis can provide important information for the control of antifungal resistance as well as distribution and susceptibility profiles in populations.https://ijph.tums.ac.ir/index.php/ijph/article/view/6264Candida parapsilosisAntifungal susceptibilityResistantIran |
| spellingShingle | Ensieh LOTFALI Parivash KORDBACHEH Hossein MIRHENDI Farideh ZAINI Ali GHAJARI Rasoul MOHAMMADI Fatemeh NOORBAKHSH Maryam MOAZENI Aliakbar FALLAHI Sassan REZAIE Antifungal Susceptibility Analysis of Clinical Isolates of Candida parapsilosis in Iran Iranian Journal of Public Health Candida parapsilosis Antifungal susceptibility Resistant Iran |
| title | Antifungal Susceptibility Analysis of Clinical Isolates of Candida parapsilosis in Iran |
| title_full | Antifungal Susceptibility Analysis of Clinical Isolates of Candida parapsilosis in Iran |
| title_fullStr | Antifungal Susceptibility Analysis of Clinical Isolates of Candida parapsilosis in Iran |
| title_full_unstemmed | Antifungal Susceptibility Analysis of Clinical Isolates of Candida parapsilosis in Iran |
| title_short | Antifungal Susceptibility Analysis of Clinical Isolates of Candida parapsilosis in Iran |
| title_sort | antifungal susceptibility analysis of clinical isolates of candida parapsilosis in iran |
| topic | Candida parapsilosis Antifungal susceptibility Resistant Iran |
| url | https://ijph.tums.ac.ir/index.php/ijph/article/view/6264 |
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