Femtosecond infrared spectroscopy of channelrhodopsin-1 chromophore isomerization
Vibrational dynamics of the retinal all-trans to 13-cis photoisomerization in channelrhodopsin-1 from Chlamydomonas augustae (CaChR1) was investigated by femtosecond visible pump mid-IR probe spectroscopy. After photoexcitation, the transient infrared absorption of C-C stretching modes was detected....
| Main Authors: | , , , , , |
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| Format: | Article |
| Language: | English |
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AIP Publishing LLC and ACA
2016-07-01
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| Series: | Structural Dynamics |
| Online Access: | http://dx.doi.org/10.1063/1.4948338 |
| _version_ | 1819031926125625344 |
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| author | T. Stensitzki Y. Yang V. Muders R. Schlesinger J. Heberle K. Heyne |
| author_facet | T. Stensitzki Y. Yang V. Muders R. Schlesinger J. Heberle K. Heyne |
| author_sort | T. Stensitzki |
| collection | DOAJ |
| description | Vibrational dynamics of the retinal all-trans to 13-cis photoisomerization in channelrhodopsin-1 from Chlamydomonas augustae (CaChR1) was investigated by femtosecond visible pump mid-IR probe spectroscopy. After photoexcitation, the transient infrared absorption of C-C stretching modes was detected. The formation of the 13-cis photoproduct marker band at 1193 cm−1 was observed within the time resolution of 0.3 ps. We estimated the photoisomerization yield to (60 ± 6) %. We found additional time constants of (0.55 ± 0.05) ps and (6 ± 1) ps, assigned to cooling, and cooling processes with a back-reaction pathway. An additional bleaching band demonstrates the ground-state heterogeneity of retinal. |
| first_indexed | 2024-12-21T06:53:48Z |
| format | Article |
| id | doaj.art-81f0c3d225fc47a4bc3a1afec9c8ccb0 |
| institution | Directory Open Access Journal |
| issn | 2329-7778 |
| language | English |
| last_indexed | 2024-12-21T06:53:48Z |
| publishDate | 2016-07-01 |
| publisher | AIP Publishing LLC and ACA |
| record_format | Article |
| series | Structural Dynamics |
| spelling | doaj.art-81f0c3d225fc47a4bc3a1afec9c8ccb02022-12-21T19:12:24ZengAIP Publishing LLC and ACAStructural Dynamics2329-77782016-07-0134043208043208-810.1063/1.4948338021693SDYFemtosecond infrared spectroscopy of channelrhodopsin-1 chromophore isomerizationT. Stensitzki0Y. Yang1V. Muders2R. Schlesinger3J. Heberle4K. Heyne5 Department of Physics, Institute of Experimental Physics, Freie Universität Berlin, Arnimallee 14, 14195 Berlin, Germany Department of Physics, Institute of Experimental Physics, Freie Universität Berlin, Arnimallee 14, 14195 Berlin, Germany Genetic Biophysics, Department of Physics, Freie Universität Berlin, Arnimallee 14, 14195 Berlin, Germany Genetic Biophysics, Department of Physics, Freie Universität Berlin, Arnimallee 14, 14195 Berlin, Germany Experimental Molecular Biophysics, Department of Physics, Freie Universität Berlin, Arnimallee 14, 14195 Berlin, Germany Department of Physics, Institute of Experimental Physics, Freie Universität Berlin, Arnimallee 14, 14195 Berlin, GermanyVibrational dynamics of the retinal all-trans to 13-cis photoisomerization in channelrhodopsin-1 from Chlamydomonas augustae (CaChR1) was investigated by femtosecond visible pump mid-IR probe spectroscopy. After photoexcitation, the transient infrared absorption of C-C stretching modes was detected. The formation of the 13-cis photoproduct marker band at 1193 cm−1 was observed within the time resolution of 0.3 ps. We estimated the photoisomerization yield to (60 ± 6) %. We found additional time constants of (0.55 ± 0.05) ps and (6 ± 1) ps, assigned to cooling, and cooling processes with a back-reaction pathway. An additional bleaching band demonstrates the ground-state heterogeneity of retinal.http://dx.doi.org/10.1063/1.4948338 |
| spellingShingle | T. Stensitzki Y. Yang V. Muders R. Schlesinger J. Heberle K. Heyne Femtosecond infrared spectroscopy of channelrhodopsin-1 chromophore isomerization Structural Dynamics |
| title | Femtosecond infrared spectroscopy of channelrhodopsin-1 chromophore isomerization |
| title_full | Femtosecond infrared spectroscopy of channelrhodopsin-1 chromophore isomerization |
| title_fullStr | Femtosecond infrared spectroscopy of channelrhodopsin-1 chromophore isomerization |
| title_full_unstemmed | Femtosecond infrared spectroscopy of channelrhodopsin-1 chromophore isomerization |
| title_short | Femtosecond infrared spectroscopy of channelrhodopsin-1 chromophore isomerization |
| title_sort | femtosecond infrared spectroscopy of channelrhodopsin 1 chromophore isomerization |
| url | http://dx.doi.org/10.1063/1.4948338 |
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