IHC characteristics of stem cell markers in PDAC with different degrees of differentiation
Introduction. Cancer stem cells (CSCs) play a key role in the development, progression and metastasis of cancer. Cell surface markers, in particular CD44 and EpCAM, are used to identify CSCs. Marker expression patterns depend on the stage of progression and differentiation. Materials and methods. H...
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Kazimierz Wielki University
2022-06-01
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| Series: | Journal of Education, Health and Sport |
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| Online Access: | https://apcz.umk.pl/JEHS/article/view/38817 |
| _version_ | 1818116931624894464 |
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| author | V. Kabachenko M. Shishkin |
| author_facet | V. Kabachenko M. Shishkin |
| author_sort | V. Kabachenko |
| collection | DOAJ |
| description |
Introduction. Cancer stem cells (CSCs) play a key role in the development, progression and metastasis of cancer. Cell surface markers, in particular CD44 and EpCAM, are used to identify CSCs. Marker expression patterns depend on the stage of progression and differentiation. Materials and methods. Histopathological and IHC studies of 49 cases of the pancreas ductal adenocarcinoma (PDAC) of G2, G3 degrees of differentiation. Results of IHC reactions with antibodies against CD44 and EpCAM were estimated by photo digital morphometry and were expressed in immunostained cells relative area (%). Results. Adenocarinoma of the pancreas is characterized by a moderate level of expression of cell surface markers CSC - CD44 and EpCAM. The expression level of CD44 in the І gr. - Me = 34.11% (Q1 = 31.03; Q3 = 38.39), in the II gr. - Me = 43.17% (Q1 = 39.83; Q3 = 47.04), significantly higher than in the I gr. (Kruskal-Wallis test p<0.05). The expression level of EpCAM in the I gr. - Ме=26,10% (Q1=20,42; Q3=31,89), in the II gr. - Ме=31,07% (Q1=27,03; Q3=34,00) (p>0,05). In both groups, the relative area of EpCAM was significantly lower than CD44 (Kruskal-Wallis test p<0.05). Correlation analysis (Pearson's ratio) showed a weak negative relationship between markers at G2 (r = -0.17, p>0.05), at G3 - a weak positive (r = + 0.06, p>0.05).
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| last_indexed | 2024-12-11T04:30:21Z |
| publishDate | 2022-06-01 |
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| series | Journal of Education, Health and Sport |
| spelling | doaj.art-81f28d1baffe44c58a5abec12923043f2022-12-22T01:20:53ZengKazimierz Wielki UniversityJournal of Education, Health and Sport2391-83062022-06-0112610.12775/JEHS.2022.12.06.023IHC characteristics of stem cell markers in PDAC with different degrees of differentiationV. Kabachenko0M. Shishkin1Zaporizhzhia State Medical UniversityZaporizhzhia State Medical University Introduction. Cancer stem cells (CSCs) play a key role in the development, progression and metastasis of cancer. Cell surface markers, in particular CD44 and EpCAM, are used to identify CSCs. Marker expression patterns depend on the stage of progression and differentiation. Materials and methods. Histopathological and IHC studies of 49 cases of the pancreas ductal adenocarcinoma (PDAC) of G2, G3 degrees of differentiation. Results of IHC reactions with antibodies against CD44 and EpCAM were estimated by photo digital morphometry and were expressed in immunostained cells relative area (%). Results. Adenocarinoma of the pancreas is characterized by a moderate level of expression of cell surface markers CSC - CD44 and EpCAM. The expression level of CD44 in the І gr. - Me = 34.11% (Q1 = 31.03; Q3 = 38.39), in the II gr. - Me = 43.17% (Q1 = 39.83; Q3 = 47.04), significantly higher than in the I gr. (Kruskal-Wallis test p<0.05). The expression level of EpCAM in the I gr. - Ме=26,10% (Q1=20,42; Q3=31,89), in the II gr. - Ме=31,07% (Q1=27,03; Q3=34,00) (p>0,05). In both groups, the relative area of EpCAM was significantly lower than CD44 (Kruskal-Wallis test p<0.05). Correlation analysis (Pearson's ratio) showed a weak negative relationship between markers at G2 (r = -0.17, p>0.05), at G3 - a weak positive (r = + 0.06, p>0.05). https://apcz.umk.pl/JEHS/article/view/38817PancreasDuctal adenocarcinomaCancer stem cellsEpCAMCD44 |
| spellingShingle | V. Kabachenko M. Shishkin IHC characteristics of stem cell markers in PDAC with different degrees of differentiation Journal of Education, Health and Sport Pancreas Ductal adenocarcinoma Cancer stem cells EpCAM CD44 |
| title | IHC characteristics of stem cell markers in PDAC with different degrees of differentiation |
| title_full | IHC characteristics of stem cell markers in PDAC with different degrees of differentiation |
| title_fullStr | IHC characteristics of stem cell markers in PDAC with different degrees of differentiation |
| title_full_unstemmed | IHC characteristics of stem cell markers in PDAC with different degrees of differentiation |
| title_short | IHC characteristics of stem cell markers in PDAC with different degrees of differentiation |
| title_sort | ihc characteristics of stem cell markers in pdac with different degrees of differentiation |
| topic | Pancreas Ductal adenocarcinoma Cancer stem cells EpCAM CD44 |
| url | https://apcz.umk.pl/JEHS/article/view/38817 |
| work_keys_str_mv | AT vkabachenko ihccharacteristicsofstemcellmarkersinpdacwithdifferentdegreesofdifferentiation AT mshishkin ihccharacteristicsofstemcellmarkersinpdacwithdifferentdegreesofdifferentiation |