In Vitro and In Vivo Antifungal Activity of Sorbicillinoids Produced by <i>Trichoderma longibrachiatum</i>
In the search for antifungal agents from marine resources, we recently found that the culture filtrate of <i>Trichoderma longibrachiatum</i> SFC100166 effectively suppressed the development of tomato gray mold, rice blast, and tomato late blight. The culture filtrate was then successivel...
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MDPI AG
2021-05-01
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| Series: | Journal of Fungi |
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| Online Access: | https://www.mdpi.com/2309-608X/7/6/428 |
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| author | Men Thi Ngo Minh Van Nguyen Jae Woo Han Myung Soo Park Hun Kim Gyung Ja Choi |
| author_facet | Men Thi Ngo Minh Van Nguyen Jae Woo Han Myung Soo Park Hun Kim Gyung Ja Choi |
| author_sort | Men Thi Ngo |
| collection | DOAJ |
| description | In the search for antifungal agents from marine resources, we recently found that the culture filtrate of <i>Trichoderma longibrachiatum</i> SFC100166 effectively suppressed the development of tomato gray mold, rice blast, and tomato late blight. The culture filtrate was then successively extracted with ethyl acetate and n-butanol to identify the fungicidal metabolites. Consequently, a new compound, spirosorbicillinol D (<b>1</b>), and a new natural compound, 2′,3′-dihydro-epoxysorbicillinol (<b>2</b>), together with 11 known compounds (<b>3</b>–<b>13</b>), were obtained from the solvent extracts. The chemical structures were determined by spectroscopic analyses and comparison with literature values. The results of the in vitro antifungal assay showed that of the tested fungal pathogens, <i>Phytophthora infestans</i> was the fungus most sensitive to the isolated compounds, with MIC values ranging from 6.3 to 400 µg/mL, except for trichotetronine (<b>9</b>) and trichodimerol (<b>10</b>). When tomato plants were treated with the representative compounds (<b>4</b>, <b>6</b>, <b>7</b>, and <b>11</b>), bisvertinolone (<b>6</b>) strongly reduced the development of tomato late blight disease compared to the untreated control. Taken together, our results revealed that the culture filtrate of <i>T. longibrachiatum</i> SFC100166 and its metabolites could be useful sources for the development of new natural agents to control late blight caused by <i>P. infestans</i>. |
| first_indexed | 2024-03-10T10:55:43Z |
| format | Article |
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| issn | 2309-608X |
| language | English |
| last_indexed | 2024-03-10T10:55:43Z |
| publishDate | 2021-05-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Journal of Fungi |
| spelling | doaj.art-81f2d8b1dccc44e9a7d12bccb9d603862023-11-21T21:55:05ZengMDPI AGJournal of Fungi2309-608X2021-05-017642810.3390/jof7060428In Vitro and In Vivo Antifungal Activity of Sorbicillinoids Produced by <i>Trichoderma longibrachiatum</i>Men Thi Ngo0Minh Van Nguyen1Jae Woo Han2Myung Soo Park3Hun Kim4Gyung Ja Choi5Center for Eco-Friendly New Materials, Korea Research Institute of Chemical Technology, Daejeon 34114, KoreaCenter for Eco-Friendly New Materials, Korea Research Institute of Chemical Technology, Daejeon 34114, KoreaCenter for Eco-Friendly New Materials, Korea Research Institute of Chemical Technology, Daejeon 34114, KoreaSchool of Biological Sciences, Seoul National University, Seoul 08826, KoreaCenter for Eco-Friendly New Materials, Korea Research Institute of Chemical Technology, Daejeon 34114, KoreaCenter for Eco-Friendly New Materials, Korea Research Institute of Chemical Technology, Daejeon 34114, KoreaIn the search for antifungal agents from marine resources, we recently found that the culture filtrate of <i>Trichoderma longibrachiatum</i> SFC100166 effectively suppressed the development of tomato gray mold, rice blast, and tomato late blight. The culture filtrate was then successively extracted with ethyl acetate and n-butanol to identify the fungicidal metabolites. Consequently, a new compound, spirosorbicillinol D (<b>1</b>), and a new natural compound, 2′,3′-dihydro-epoxysorbicillinol (<b>2</b>), together with 11 known compounds (<b>3</b>–<b>13</b>), were obtained from the solvent extracts. The chemical structures were determined by spectroscopic analyses and comparison with literature values. The results of the in vitro antifungal assay showed that of the tested fungal pathogens, <i>Phytophthora infestans</i> was the fungus most sensitive to the isolated compounds, with MIC values ranging from 6.3 to 400 µg/mL, except for trichotetronine (<b>9</b>) and trichodimerol (<b>10</b>). When tomato plants were treated with the representative compounds (<b>4</b>, <b>6</b>, <b>7</b>, and <b>11</b>), bisvertinolone (<b>6</b>) strongly reduced the development of tomato late blight disease compared to the untreated control. Taken together, our results revealed that the culture filtrate of <i>T. longibrachiatum</i> SFC100166 and its metabolites could be useful sources for the development of new natural agents to control late blight caused by <i>P. infestans</i>.https://www.mdpi.com/2309-608X/7/6/428<i>Trichoderma longibrachiatum</i>sorbicillinoidantifungal compoundplant pathogen |
| spellingShingle | Men Thi Ngo Minh Van Nguyen Jae Woo Han Myung Soo Park Hun Kim Gyung Ja Choi In Vitro and In Vivo Antifungal Activity of Sorbicillinoids Produced by <i>Trichoderma longibrachiatum</i> Journal of Fungi <i>Trichoderma longibrachiatum</i> sorbicillinoid antifungal compound plant pathogen |
| title | In Vitro and In Vivo Antifungal Activity of Sorbicillinoids Produced by <i>Trichoderma longibrachiatum</i> |
| title_full | In Vitro and In Vivo Antifungal Activity of Sorbicillinoids Produced by <i>Trichoderma longibrachiatum</i> |
| title_fullStr | In Vitro and In Vivo Antifungal Activity of Sorbicillinoids Produced by <i>Trichoderma longibrachiatum</i> |
| title_full_unstemmed | In Vitro and In Vivo Antifungal Activity of Sorbicillinoids Produced by <i>Trichoderma longibrachiatum</i> |
| title_short | In Vitro and In Vivo Antifungal Activity of Sorbicillinoids Produced by <i>Trichoderma longibrachiatum</i> |
| title_sort | in vitro and in vivo antifungal activity of sorbicillinoids produced by i trichoderma longibrachiatum i |
| topic | <i>Trichoderma longibrachiatum</i> sorbicillinoid antifungal compound plant pathogen |
| url | https://www.mdpi.com/2309-608X/7/6/428 |
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