Testin on Atherosclerosis in Rabbits
Background:. The expression of TES, a novel tumor suppressor gene, is found to be down-regulated in the left anterior descending aorta of patients with coronary artery disease (CAD) compared with non-CAD subjects. This study aimed to investigate the expression of TES during the development of athero...
| Main Authors: | , , , , , |
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| Format: | Article |
| Language: | English |
| Published: |
Wolters Kluwer
2015-06-01
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| Series: | Chinese Medical Journal |
| Online Access: | http://journals.lww.com/10.4103/0366-6999.158370 |
| _version_ | 1827780712419295232 |
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| author | Yue Zhang Meng Yuan Hong-Min Li Mi Lao Zhao Xu Guang-Ping Li |
| author_facet | Yue Zhang Meng Yuan Hong-Min Li Mi Lao Zhao Xu Guang-Ping Li |
| author_sort | Yue Zhang |
| collection | DOAJ |
| description | Background:. The expression of TES, a novel tumor suppressor gene, is found to be down-regulated in the left anterior descending aorta of patients with coronary artery disease (CAD) compared with non-CAD subjects. This study aimed to investigate the expression of TES during the development of atherosclerosis in rabbits.
Methods:. Thirty-two New Zealand rabbits were randomly divided into a normal diet (ND) and high-fat diet (HFD) groups. Body weight and serum lipid levels were measured at 0, 4, and 12 weeks after diet treatment. The degree of atherosclerosis in thoracic aortas was analyzed by histological examinations. The expression of Testin in the tissue samples was inspected via immunohistochemical and immunofluorescence confocal microscopy. Real time-polymerase chain reaction and Western blot analysis were performed to evaluate the expression of TES/Testin at mRNA and protein levels in the aortic tissues.
Results:. After 12 weeks postenrollment, rabbits in HFD group had a higher level of serum lipids and atherosclerotic plaque compared to ND group (P < 0.05). Testin expression was detected at high levels in the endothelium and a weak expression on the subendothelium area. The expression of TES mRNA was markedly reduced by 10-fold in the aortic tissues in the HFD group compared with the ND group (P = 0.015), and the protein level was also significantly decreased in the HFD group (P < 0.05).
Conclusions:. Reduced TES/Testin expression is associated with the development of atherosclerosis, implicating a potentially important role in the pathogenesis of atherosclerosis. |
| first_indexed | 2024-03-11T15:08:53Z |
| format | Article |
| id | doaj.art-81f5e670ad9c423e93d13703dcfaa9ef |
| institution | Directory Open Access Journal |
| issn | 0366-6999 2542-5641 |
| language | English |
| last_indexed | 2024-03-11T15:08:53Z |
| publishDate | 2015-06-01 |
| publisher | Wolters Kluwer |
| record_format | Article |
| series | Chinese Medical Journal |
| spelling | doaj.art-81f5e670ad9c423e93d13703dcfaa9ef2023-10-30T03:37:29ZengWolters KluwerChinese Medical Journal0366-69992542-56412015-06-01128121662166510.4103/0366-6999.158370cm9-128-1662Testin on Atherosclerosis in RabbitsYue Zhang0Meng Yuan1Hong-Min Li2Mi Lao3Zhao Xu4Guang-Ping Li51 Department of Cardiology, Tianjin Key Laboratory of Ionic-Molecular Function of Cardiovascular Disease (Key Lab-TIC), Tianjin Institute of Cardiology, Second Hospital of Tianjin Medical University, Tianjin 300211, China2 Collaborative Innovation Center of Tianjin for Medical Epigenetics, Department of Physiology and Pathophysiology, Tianjin Medical University, Tianjin 300070, China1 Department of Cardiology, Tianjin Key Laboratory of Ionic-Molecular Function of Cardiovascular Disease (Key Lab-TIC), Tianjin Institute of Cardiology, Second Hospital of Tianjin Medical University, Tianjin 300211, China3 Department of Cardiology, Binzhou People's Hospital, Binzhou, Shandong 256600, China1 Department of Cardiology, Tianjin Key Laboratory of Ionic-Molecular Function of Cardiovascular Disease (Key Lab-TIC), Tianjin Institute of Cardiology, Second Hospital of Tianjin Medical University, Tianjin 300211, China1 Department of Cardiology, Tianjin Key Laboratory of Ionic-Molecular Function of Cardiovascular Disease (Key Lab-TIC), Tianjin Institute of Cardiology, Second Hospital of Tianjin Medical University, Tianjin 300211, ChinaBackground:. The expression of TES, a novel tumor suppressor gene, is found to be down-regulated in the left anterior descending aorta of patients with coronary artery disease (CAD) compared with non-CAD subjects. This study aimed to investigate the expression of TES during the development of atherosclerosis in rabbits. Methods:. Thirty-two New Zealand rabbits were randomly divided into a normal diet (ND) and high-fat diet (HFD) groups. Body weight and serum lipid levels were measured at 0, 4, and 12 weeks after diet treatment. The degree of atherosclerosis in thoracic aortas was analyzed by histological examinations. The expression of Testin in the tissue samples was inspected via immunohistochemical and immunofluorescence confocal microscopy. Real time-polymerase chain reaction and Western blot analysis were performed to evaluate the expression of TES/Testin at mRNA and protein levels in the aortic tissues. Results:. After 12 weeks postenrollment, rabbits in HFD group had a higher level of serum lipids and atherosclerotic plaque compared to ND group (P < 0.05). Testin expression was detected at high levels in the endothelium and a weak expression on the subendothelium area. The expression of TES mRNA was markedly reduced by 10-fold in the aortic tissues in the HFD group compared with the ND group (P = 0.015), and the protein level was also significantly decreased in the HFD group (P < 0.05). Conclusions:. Reduced TES/Testin expression is associated with the development of atherosclerosis, implicating a potentially important role in the pathogenesis of atherosclerosis.http://journals.lww.com/10.4103/0366-6999.158370 |
| spellingShingle | Yue Zhang Meng Yuan Hong-Min Li Mi Lao Zhao Xu Guang-Ping Li Testin on Atherosclerosis in Rabbits Chinese Medical Journal |
| title | Testin on Atherosclerosis in Rabbits |
| title_full | Testin on Atherosclerosis in Rabbits |
| title_fullStr | Testin on Atherosclerosis in Rabbits |
| title_full_unstemmed | Testin on Atherosclerosis in Rabbits |
| title_short | Testin on Atherosclerosis in Rabbits |
| title_sort | testin on atherosclerosis in rabbits |
| url | http://journals.lww.com/10.4103/0366-6999.158370 |
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