Characterization and Functional Analysis of Toll Receptor Genes during Antibacterial Immunity in the Green Peach Aphid <i>Myzus persicae</i> (Sulzer)
The insect Toll receptor is one of the key members of the Toll signaling pathway, which plays an indispensable role in insect resistance to pathogen infection. Herein, we cloned and characterized five Toll receptor genes from <i>Myzus persicae</i> (Sulzer), which were found to be highly...
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MDPI AG
2023-03-01
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| Series: | Insects |
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| Online Access: | https://www.mdpi.com/2075-4450/14/3/275 |
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| author | Li He Chao Zhang Hong Yang Bo Ding Han-Zhi Yang Sen-Wen Zhang |
| author_facet | Li He Chao Zhang Hong Yang Bo Ding Han-Zhi Yang Sen-Wen Zhang |
| author_sort | Li He |
| collection | DOAJ |
| description | The insect Toll receptor is one of the key members of the Toll signaling pathway, which plays an indispensable role in insect resistance to pathogen infection. Herein, we cloned and characterized five Toll receptor genes from <i>Myzus persicae</i> (Sulzer), which were found to be highly expressed in the first-instar nymphs and adults (both wingless and winged) at different developmental stages. Expressions of MpToll genes were highest in the head, followed by the epidermis. High transcription levels were also found in embryos. Expressions of these genes showed different degrees of positive responses to infection by <i>Escherichia coli</i> and <i>Staphylococcus aureus</i>. The expression of <i>MpToll6</i>-<i>1</i> and <i>MpToll7</i> significantly increased after infection with <i>E. coli</i>, whereas the expression of <i>MpToll</i>, <i>MpToll6</i>, <i>MpToll6</i>-<i>1</i>, and <i>MpTollo</i> continuously increased after infection with <i>S. aureus</i>. RNA interference-mediated suppressed expression of these genes resulted in a significant increase in the mortality of <i>M. persicae</i> after infection with the two bacterial species compared with that in the control group. These results suggest that MpToll genes play vital roles in the defense response of <i>M. persicae</i> against bacteria. |
| first_indexed | 2024-03-11T06:23:07Z |
| format | Article |
| id | doaj.art-81f8aecbbf5b41da8902e70ee30b039f |
| institution | Directory Open Access Journal |
| issn | 2075-4450 |
| language | English |
| last_indexed | 2024-03-11T06:23:07Z |
| publishDate | 2023-03-01 |
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| series | Insects |
| spelling | doaj.art-81f8aecbbf5b41da8902e70ee30b039f2023-11-17T11:46:21ZengMDPI AGInsects2075-44502023-03-0114327510.3390/insects14030275Characterization and Functional Analysis of Toll Receptor Genes during Antibacterial Immunity in the Green Peach Aphid <i>Myzus persicae</i> (Sulzer)Li He0Chao Zhang1Hong Yang2Bo Ding3Han-Zhi Yang4Sen-Wen Zhang5Guizhou Provincial Key Laboratory for Agricultural Pest Management of Mountainous Region, Institute of Entomology, Guizhou University, Guiyang 550025, ChinaGuizhou Provincial Key Laboratory for Agricultural Pest Management of Mountainous Region, Institute of Entomology, Guizhou University, Guiyang 550025, ChinaGuizhou Provincial Key Laboratory for Agricultural Pest Management of Mountainous Region, Institute of Entomology, Guizhou University, Guiyang 550025, ChinaGuizhou Provincial Key Laboratory for Agricultural Pest Management of Mountainous Region, Institute of Entomology, Guizhou University, Guiyang 550025, ChinaGuizhou Provincial Key Laboratory for Agricultural Pest Management of Mountainous Region, Institute of Entomology, Guizhou University, Guiyang 550025, ChinaGuizhou Provincial Key Laboratory for Agricultural Pest Management of Mountainous Region, Institute of Entomology, Guizhou University, Guiyang 550025, ChinaThe insect Toll receptor is one of the key members of the Toll signaling pathway, which plays an indispensable role in insect resistance to pathogen infection. Herein, we cloned and characterized five Toll receptor genes from <i>Myzus persicae</i> (Sulzer), which were found to be highly expressed in the first-instar nymphs and adults (both wingless and winged) at different developmental stages. Expressions of MpToll genes were highest in the head, followed by the epidermis. High transcription levels were also found in embryos. Expressions of these genes showed different degrees of positive responses to infection by <i>Escherichia coli</i> and <i>Staphylococcus aureus</i>. The expression of <i>MpToll6</i>-<i>1</i> and <i>MpToll7</i> significantly increased after infection with <i>E. coli</i>, whereas the expression of <i>MpToll</i>, <i>MpToll6</i>, <i>MpToll6</i>-<i>1</i>, and <i>MpTollo</i> continuously increased after infection with <i>S. aureus</i>. RNA interference-mediated suppressed expression of these genes resulted in a significant increase in the mortality of <i>M. persicae</i> after infection with the two bacterial species compared with that in the control group. These results suggest that MpToll genes play vital roles in the defense response of <i>M. persicae</i> against bacteria.https://www.mdpi.com/2075-4450/14/3/275<i>Myzus persicae</i>innate immunityToll receptorbacterial infectionRNA interference |
| spellingShingle | Li He Chao Zhang Hong Yang Bo Ding Han-Zhi Yang Sen-Wen Zhang Characterization and Functional Analysis of Toll Receptor Genes during Antibacterial Immunity in the Green Peach Aphid <i>Myzus persicae</i> (Sulzer) Insects <i>Myzus persicae</i> innate immunity Toll receptor bacterial infection RNA interference |
| title | Characterization and Functional Analysis of Toll Receptor Genes during Antibacterial Immunity in the Green Peach Aphid <i>Myzus persicae</i> (Sulzer) |
| title_full | Characterization and Functional Analysis of Toll Receptor Genes during Antibacterial Immunity in the Green Peach Aphid <i>Myzus persicae</i> (Sulzer) |
| title_fullStr | Characterization and Functional Analysis of Toll Receptor Genes during Antibacterial Immunity in the Green Peach Aphid <i>Myzus persicae</i> (Sulzer) |
| title_full_unstemmed | Characterization and Functional Analysis of Toll Receptor Genes during Antibacterial Immunity in the Green Peach Aphid <i>Myzus persicae</i> (Sulzer) |
| title_short | Characterization and Functional Analysis of Toll Receptor Genes during Antibacterial Immunity in the Green Peach Aphid <i>Myzus persicae</i> (Sulzer) |
| title_sort | characterization and functional analysis of toll receptor genes during antibacterial immunity in the green peach aphid i myzus persicae i sulzer |
| topic | <i>Myzus persicae</i> innate immunity Toll receptor bacterial infection RNA interference |
| url | https://www.mdpi.com/2075-4450/14/3/275 |
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