Quorum Sensing in <i>Halorubrum saccharovorum</i> Facilitates Cross-Domain Signaling between Archaea and Bacteria
Quorum Sensing (QS) is a well-studied intercellular communication mechanism in bacteria, regulating collective behaviors such as biofilm formation, virulence, and antibiotic resistance. However, cell–cell signaling in haloarchaea remains largely unexplored. The coexistence of bacteria and archaea in...
| Main Authors: | , , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
MDPI AG
2023-05-01
|
| Series: | Microorganisms |
| Subjects: | |
| Online Access: | https://www.mdpi.com/2076-2607/11/5/1271 |
| _version_ | 1797598996411187200 |
|---|---|
| author | Thomas P. Thompson Alessandro Busetti Brendan F. Gilmore |
| author_facet | Thomas P. Thompson Alessandro Busetti Brendan F. Gilmore |
| author_sort | Thomas P. Thompson |
| collection | DOAJ |
| description | Quorum Sensing (QS) is a well-studied intercellular communication mechanism in bacteria, regulating collective behaviors such as biofilm formation, virulence, and antibiotic resistance. However, cell–cell signaling in haloarchaea remains largely unexplored. The coexistence of bacteria and archaea in various environments, coupled with the known cell–cell signaling mechanisms in both prokaryotic and eukaryotic microorganisms and the presence of cell–cell signaling mechanisms in both prokaryotic and eukaryotic microorganisms, suggests a possibility for haloarchaea to possess analogous cell–cell signaling or QS systems. Recently, N-acylhomoserine lactone (AHL)-like compounds were identified in haloarchaea; yet, their precise role—for example, persister cell formation—remains ambiguous. This study investigated the capacity of crude supernatant extract from the haloarchaeon <i>Halorubrum saccharovorum</i> CSM52 to stimulate bacterial AHL-dependent QS phenotypes using bioreporter strains. Our findings reveal that these crude extracts induced several AHL-dependent bioreporters and modulated pyocyanin and pyoverdine production in <i>Pseudomonas aeruginosa</i>. Importantly, our study suggests cross-domain communication between archaea and bacterial pathogens, providing evidence for archaea potentially influencing bacterial virulence. Using Thin Layer Chromatography overlay assays, lactonolysis, and colorimetric quantification, the bioactive compound was inferred to be a chemically modified AHL-like compound or a diketopiperazine-like molecule, potentially involved in biofilm formation in <i>H. saccharovorum</i> CSM52. This study offers new insights into putative QS mechanisms in haloarchaea and their potential role in interspecies communication and coordination, thereby enriching our understanding of microbial interactions in diverse environments. |
| first_indexed | 2024-03-11T03:28:31Z |
| format | Article |
| id | doaj.art-81fb0f41756d4cfbb7c2c804a1e91934 |
| institution | Directory Open Access Journal |
| issn | 2076-2607 |
| language | English |
| last_indexed | 2024-03-11T03:28:31Z |
| publishDate | 2023-05-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Microorganisms |
| spelling | doaj.art-81fb0f41756d4cfbb7c2c804a1e919342023-11-18T02:34:18ZengMDPI AGMicroorganisms2076-26072023-05-01115127110.3390/microorganisms11051271Quorum Sensing in <i>Halorubrum saccharovorum</i> Facilitates Cross-Domain Signaling between Archaea and BacteriaThomas P. Thompson0Alessandro Busetti1Brendan F. Gilmore2Biofilm Research Group, School of Pharmacy, Queen’s University Belfast, 97 Lisburn Road, Belfast BT9 7BL, UKBiofilm Research Group, School of Pharmacy, Queen’s University Belfast, 97 Lisburn Road, Belfast BT9 7BL, UKBiofilm Research Group, School of Pharmacy, Queen’s University Belfast, 97 Lisburn Road, Belfast BT9 7BL, UKQuorum Sensing (QS) is a well-studied intercellular communication mechanism in bacteria, regulating collective behaviors such as biofilm formation, virulence, and antibiotic resistance. However, cell–cell signaling in haloarchaea remains largely unexplored. The coexistence of bacteria and archaea in various environments, coupled with the known cell–cell signaling mechanisms in both prokaryotic and eukaryotic microorganisms and the presence of cell–cell signaling mechanisms in both prokaryotic and eukaryotic microorganisms, suggests a possibility for haloarchaea to possess analogous cell–cell signaling or QS systems. Recently, N-acylhomoserine lactone (AHL)-like compounds were identified in haloarchaea; yet, their precise role—for example, persister cell formation—remains ambiguous. This study investigated the capacity of crude supernatant extract from the haloarchaeon <i>Halorubrum saccharovorum</i> CSM52 to stimulate bacterial AHL-dependent QS phenotypes using bioreporter strains. Our findings reveal that these crude extracts induced several AHL-dependent bioreporters and modulated pyocyanin and pyoverdine production in <i>Pseudomonas aeruginosa</i>. Importantly, our study suggests cross-domain communication between archaea and bacterial pathogens, providing evidence for archaea potentially influencing bacterial virulence. Using Thin Layer Chromatography overlay assays, lactonolysis, and colorimetric quantification, the bioactive compound was inferred to be a chemically modified AHL-like compound or a diketopiperazine-like molecule, potentially involved in biofilm formation in <i>H. saccharovorum</i> CSM52. This study offers new insights into putative QS mechanisms in haloarchaea and their potential role in interspecies communication and coordination, thereby enriching our understanding of microbial interactions in diverse environments.https://www.mdpi.com/2076-2607/11/5/1271quorum sensingarchaeabiofilmN-acyl homoserine lactoneshalophiles |
| spellingShingle | Thomas P. Thompson Alessandro Busetti Brendan F. Gilmore Quorum Sensing in <i>Halorubrum saccharovorum</i> Facilitates Cross-Domain Signaling between Archaea and Bacteria Microorganisms quorum sensing archaea biofilm N-acyl homoserine lactones halophiles |
| title | Quorum Sensing in <i>Halorubrum saccharovorum</i> Facilitates Cross-Domain Signaling between Archaea and Bacteria |
| title_full | Quorum Sensing in <i>Halorubrum saccharovorum</i> Facilitates Cross-Domain Signaling between Archaea and Bacteria |
| title_fullStr | Quorum Sensing in <i>Halorubrum saccharovorum</i> Facilitates Cross-Domain Signaling between Archaea and Bacteria |
| title_full_unstemmed | Quorum Sensing in <i>Halorubrum saccharovorum</i> Facilitates Cross-Domain Signaling between Archaea and Bacteria |
| title_short | Quorum Sensing in <i>Halorubrum saccharovorum</i> Facilitates Cross-Domain Signaling between Archaea and Bacteria |
| title_sort | quorum sensing in i halorubrum saccharovorum i facilitates cross domain signaling between archaea and bacteria |
| topic | quorum sensing archaea biofilm N-acyl homoserine lactones halophiles |
| url | https://www.mdpi.com/2076-2607/11/5/1271 |
| work_keys_str_mv | AT thomaspthompson quorumsensinginihalorubrumsaccharovorumifacilitatescrossdomainsignalingbetweenarchaeaandbacteria AT alessandrobusetti quorumsensinginihalorubrumsaccharovorumifacilitatescrossdomainsignalingbetweenarchaeaandbacteria AT brendanfgilmore quorumsensinginihalorubrumsaccharovorumifacilitatescrossdomainsignalingbetweenarchaeaandbacteria |