Early Effects of Combretastatin A4 Phosphate Assessed by Anatomic and Carbogen-Based Functional Magnetic Resonance Imaging on Rat Bladder Tumors Implanted in Nude Mice
Combretastatin A4 phosphate (CA4P) causes rapid disruption of the tumor vasculature and is currently being evaluated for antivascular therapy. We describe the initial results obtained with a noninvasive multiparametric magnetic resonance imaging (MRI) approach to assess the early effects of CA4P on...
| Main Authors: | , , , , , |
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| Format: | Article |
| Language: | English |
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Elsevier
2006-07-01
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| Series: | Neoplasia: An International Journal for Oncology Research |
| Subjects: | |
| Online Access: | http://www.sciencedirect.com/science/article/pii/S147655860680014X |
| _version_ | 1811264590391017472 |
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| author | Carole D. Thomas Christine Walczak Julia Kaffy Renée Pontikis Jacqueline Jouanneau Andreas Volk |
| author_facet | Carole D. Thomas Christine Walczak Julia Kaffy Renée Pontikis Jacqueline Jouanneau Andreas Volk |
| author_sort | Carole D. Thomas |
| collection | DOAJ |
| description | Combretastatin A4 phosphate (CA4P) causes rapid disruption of the tumor vasculature and is currently being evaluated for antivascular therapy. We describe the initial results obtained with a noninvasive multiparametric magnetic resonance imaging (MRI) approach to assess the early effects of CA4P on rat bladder tumors implanted on nude mice. MRI (4.7 T) comprised a fast spin-echo sequence for growth curve assessment; a multislice multiecho sequence for T2 measurement before, 15 minutes after, 24 hours after CA4P (100 mg/kg); and a fast T2W* gradient-echo sequence to assess MR signal modification under carbogen breathing before, 35 minutes after, 24 hours after CA4P. The tumor fraction with increased T2W* signal intensity under carbogen (T+) was used to quantify CA4P effect on functional vasculature. CA4P slowed tumor growth over 24 hours and accelerated necrosis development. T+ decrease was observed already at 35 minutes post-CA4P. Early T2 increase was observed in regions becoming necrotic at 24 hours post-CA4P, as confirmed by high T2 and histology. These regions exhibited, under carbogen, a switch from T2W* signal increase before CA4P to a decrease postCA4P. The combination of carbogen-based functional MRI and T2 measurement may be useful for the early follow-up of antivascular therapy without the administration of contrast agents. |
| first_indexed | 2024-04-12T20:07:06Z |
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| institution | Directory Open Access Journal |
| issn | 1476-5586 1522-8002 |
| language | English |
| last_indexed | 2024-04-12T20:07:06Z |
| publishDate | 2006-07-01 |
| publisher | Elsevier |
| record_format | Article |
| series | Neoplasia: An International Journal for Oncology Research |
| spelling | doaj.art-81fc26b475674f6fb029de9307a4c53f2022-12-22T03:18:22ZengElsevierNeoplasia: An International Journal for Oncology Research1476-55861522-80022006-07-018758759510.1593/neo.06232Early Effects of Combretastatin A4 Phosphate Assessed by Anatomic and Carbogen-Based Functional Magnetic Resonance Imaging on Rat Bladder Tumors Implanted in Nude MiceCarole D. Thomas0Christine Walczak1Julia Kaffy2Renée Pontikis3Jacqueline Jouanneau4Andreas Volk5INSERM, U759, Orsay F-91405, FranceINSERM, U759, Orsay F-91405, FranceCNRS, UMR 176, Paris F-75005, FranceCNRS, UMR 176, Paris F-75005, FranceInstitut Curie Research Center, Paris F-75005, FranceINSERM, U759, Orsay F-91405, FranceCombretastatin A4 phosphate (CA4P) causes rapid disruption of the tumor vasculature and is currently being evaluated for antivascular therapy. We describe the initial results obtained with a noninvasive multiparametric magnetic resonance imaging (MRI) approach to assess the early effects of CA4P on rat bladder tumors implanted on nude mice. MRI (4.7 T) comprised a fast spin-echo sequence for growth curve assessment; a multislice multiecho sequence for T2 measurement before, 15 minutes after, 24 hours after CA4P (100 mg/kg); and a fast T2W* gradient-echo sequence to assess MR signal modification under carbogen breathing before, 35 minutes after, 24 hours after CA4P. The tumor fraction with increased T2W* signal intensity under carbogen (T+) was used to quantify CA4P effect on functional vasculature. CA4P slowed tumor growth over 24 hours and accelerated necrosis development. T+ decrease was observed already at 35 minutes post-CA4P. Early T2 increase was observed in regions becoming necrotic at 24 hours post-CA4P, as confirmed by high T2 and histology. These regions exhibited, under carbogen, a switch from T2W* signal increase before CA4P to a decrease postCA4P. The combination of carbogen-based functional MRI and T2 measurement may be useful for the early follow-up of antivascular therapy without the administration of contrast agents.http://www.sciencedirect.com/science/article/pii/S147655860680014XRat bladder tumorsmousecarbogenfMRIantivascular therapy |
| spellingShingle | Carole D. Thomas Christine Walczak Julia Kaffy Renée Pontikis Jacqueline Jouanneau Andreas Volk Early Effects of Combretastatin A4 Phosphate Assessed by Anatomic and Carbogen-Based Functional Magnetic Resonance Imaging on Rat Bladder Tumors Implanted in Nude Mice Neoplasia: An International Journal for Oncology Research Rat bladder tumors mouse carbogen fMRI antivascular therapy |
| title | Early Effects of Combretastatin A4 Phosphate Assessed by Anatomic and Carbogen-Based Functional Magnetic Resonance Imaging on Rat Bladder Tumors Implanted in Nude Mice |
| title_full | Early Effects of Combretastatin A4 Phosphate Assessed by Anatomic and Carbogen-Based Functional Magnetic Resonance Imaging on Rat Bladder Tumors Implanted in Nude Mice |
| title_fullStr | Early Effects of Combretastatin A4 Phosphate Assessed by Anatomic and Carbogen-Based Functional Magnetic Resonance Imaging on Rat Bladder Tumors Implanted in Nude Mice |
| title_full_unstemmed | Early Effects of Combretastatin A4 Phosphate Assessed by Anatomic and Carbogen-Based Functional Magnetic Resonance Imaging on Rat Bladder Tumors Implanted in Nude Mice |
| title_short | Early Effects of Combretastatin A4 Phosphate Assessed by Anatomic and Carbogen-Based Functional Magnetic Resonance Imaging on Rat Bladder Tumors Implanted in Nude Mice |
| title_sort | early effects of combretastatin a4 phosphate assessed by anatomic and carbogen based functional magnetic resonance imaging on rat bladder tumors implanted in nude mice |
| topic | Rat bladder tumors mouse carbogen fMRI antivascular therapy |
| url | http://www.sciencedirect.com/science/article/pii/S147655860680014X |
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