Carbapenem-resistant and cephalosporin-susceptible: a worrisome phenotype among Pseudomonas aeruginosa clinical isolates in Brazil
The mechanisms involved in the uncommon resistance phenotype, carbapenem resistance and broad-spectrum cephalosporin susceptibility, were investigated in 25 Pseudomonas aeruginosa clinical isolates that exhibited this phenotype, which were recovered from three different hospitals located in São Paul...
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| Format: | Article |
| Language: | English |
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Elsevier
2017-01-01
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| Series: | Brazilian Journal of Infectious Diseases |
| Online Access: | http://www.sciencedirect.com/science/article/pii/S1413867016305608 |
| _version_ | 1819171496198668288 |
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| author | Eloiza Helena Campana Danilo Elias Xavier Fernanda Villas-Boas Petrolini Jhonatha Rodrigo Cordeiro-Moura Maria Rita Elmor de Araujo Ana Cristina Gales |
| author_facet | Eloiza Helena Campana Danilo Elias Xavier Fernanda Villas-Boas Petrolini Jhonatha Rodrigo Cordeiro-Moura Maria Rita Elmor de Araujo Ana Cristina Gales |
| author_sort | Eloiza Helena Campana |
| collection | DOAJ |
| description | The mechanisms involved in the uncommon resistance phenotype, carbapenem resistance and broad-spectrum cephalosporin susceptibility, were investigated in 25 Pseudomonas aeruginosa clinical isolates that exhibited this phenotype, which were recovered from three different hospitals located in São Paulo, Brazil. The antimicrobial susceptibility profile was determined by CLSI broth microdilution. β-lactamase-encoding genes were investigated by PCR followed by DNA sequencing. Carbapenem hydrolysis activity was investigated by spectrophotometer and MALDI-TOF assays. The mRNA transcription level of oprD was assessed by qRT-PCR and the outer membrane proteins profile was evaluated by SDS-PAGE. Genetic relationship among P. aeruginosa isolates was assessed by PFGE. Carbapenems hydrolysis was not detected by carbapenemase assay in the carbapenem-resistant and cephalosporin-susceptible P. aueruginosa clinical isolates. OprD decreased expression was observed in all P. aeruginosa isolates by qRT-PCR. The outer membrane protein profile by SDS-PAGE suggested a change in the expression of the 46 kDa porin that could correspond to OprD porin. The isolates were clustered into 17 genotypes without predominance of a specific PFGE pattern. These results emphasize the involvement of multiple chromosomal mechanisms in carbapenem-resistance among clinical isolates of P. aeruginosa, alert for adaptation of P. aeruginosa clinical isolates under antimicrobial selective pressure and make aware of the emergence of an uncommon phenotype among P. aeruginosa clinical isolates. Keywords: Gram-negative bacilli, Carbapenem resistance, OprD porin |
| first_indexed | 2024-12-22T19:52:13Z |
| format | Article |
| id | doaj.art-81ff6e9b785047c7ad91aa59fe50d1da |
| institution | Directory Open Access Journal |
| issn | 1413-8670 |
| language | English |
| last_indexed | 2024-12-22T19:52:13Z |
| publishDate | 2017-01-01 |
| publisher | Elsevier |
| record_format | Article |
| series | Brazilian Journal of Infectious Diseases |
| spelling | doaj.art-81ff6e9b785047c7ad91aa59fe50d1da2022-12-21T18:14:30ZengElsevierBrazilian Journal of Infectious Diseases1413-86702017-01-012115762S1413-86702017000100057Carbapenem-resistant and cephalosporin-susceptible: a worrisome phenotype among Pseudomonas aeruginosa clinical isolates in BrazilEloiza Helena Campana0Danilo Elias Xavier1Fernanda Villas-Boas Petrolini2Jhonatha Rodrigo Cordeiro-Moura3Maria Rita Elmor de Araujo4Ana Cristina Gales5Universidade Federal de São Paulo, Laboratório ALERTA, Disciplina de Infectologia, São Paulo, SP, Brazil; Corresponding author.Universidade Federal de São Paulo, Laboratório ALERTA, Disciplina de Infectologia, São Paulo, SP, BrazilUniversidade Federal de São Paulo, Laboratório ALERTA, Disciplina de Infectologia, São Paulo, SP, BrazilUniversidade Federal de São Paulo, Laboratório ALERTA, Disciplina de Infectologia, São Paulo, SP, BrazilHospital Benemérita Sociedade Portuguesa de Beneficência e Hospital do Coração, São Paulo, SP, BrazilUniversidade Federal de São Paulo, Laboratório ALERTA, Disciplina de Infectologia, São Paulo, SP, BrazilThe mechanisms involved in the uncommon resistance phenotype, carbapenem resistance and broad-spectrum cephalosporin susceptibility, were investigated in 25 Pseudomonas aeruginosa clinical isolates that exhibited this phenotype, which were recovered from three different hospitals located in São Paulo, Brazil. The antimicrobial susceptibility profile was determined by CLSI broth microdilution. β-lactamase-encoding genes were investigated by PCR followed by DNA sequencing. Carbapenem hydrolysis activity was investigated by spectrophotometer and MALDI-TOF assays. The mRNA transcription level of oprD was assessed by qRT-PCR and the outer membrane proteins profile was evaluated by SDS-PAGE. Genetic relationship among P. aeruginosa isolates was assessed by PFGE. Carbapenems hydrolysis was not detected by carbapenemase assay in the carbapenem-resistant and cephalosporin-susceptible P. aueruginosa clinical isolates. OprD decreased expression was observed in all P. aeruginosa isolates by qRT-PCR. The outer membrane protein profile by SDS-PAGE suggested a change in the expression of the 46 kDa porin that could correspond to OprD porin. The isolates were clustered into 17 genotypes without predominance of a specific PFGE pattern. These results emphasize the involvement of multiple chromosomal mechanisms in carbapenem-resistance among clinical isolates of P. aeruginosa, alert for adaptation of P. aeruginosa clinical isolates under antimicrobial selective pressure and make aware of the emergence of an uncommon phenotype among P. aeruginosa clinical isolates. Keywords: Gram-negative bacilli, Carbapenem resistance, OprD porinhttp://www.sciencedirect.com/science/article/pii/S1413867016305608 |
| spellingShingle | Eloiza Helena Campana Danilo Elias Xavier Fernanda Villas-Boas Petrolini Jhonatha Rodrigo Cordeiro-Moura Maria Rita Elmor de Araujo Ana Cristina Gales Carbapenem-resistant and cephalosporin-susceptible: a worrisome phenotype among Pseudomonas aeruginosa clinical isolates in Brazil Brazilian Journal of Infectious Diseases |
| title | Carbapenem-resistant and cephalosporin-susceptible: a worrisome phenotype among Pseudomonas aeruginosa clinical isolates in Brazil |
| title_full | Carbapenem-resistant and cephalosporin-susceptible: a worrisome phenotype among Pseudomonas aeruginosa clinical isolates in Brazil |
| title_fullStr | Carbapenem-resistant and cephalosporin-susceptible: a worrisome phenotype among Pseudomonas aeruginosa clinical isolates in Brazil |
| title_full_unstemmed | Carbapenem-resistant and cephalosporin-susceptible: a worrisome phenotype among Pseudomonas aeruginosa clinical isolates in Brazil |
| title_short | Carbapenem-resistant and cephalosporin-susceptible: a worrisome phenotype among Pseudomonas aeruginosa clinical isolates in Brazil |
| title_sort | carbapenem resistant and cephalosporin susceptible a worrisome phenotype among pseudomonas aeruginosa clinical isolates in brazil |
| url | http://www.sciencedirect.com/science/article/pii/S1413867016305608 |
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