A Mechanistic Study of the Antiaging Effect of Raw-Milk Cheese Extracts
Many studies have highlighted the relationship between food and health status, with the aim of improving both disease prevention and life expectancy. Among the different food groups, fermented foods a have huge microbial biodiversity, making them an interesting source of metabolites that could exhib...
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| Format: | Article |
| Language: | English |
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MDPI AG
2021-03-01
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| Series: | Nutrients |
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| Online Access: | https://www.mdpi.com/2072-6643/13/3/897 |
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| author | Guillaume Cardin Cyril Poupet Muriel Bonnet Philippe Veisseire Isabelle Ripoche Pierre Chalard Anne Chauder Etienne Saunier Julien Priam Stéphanie Bornes Laurent Rios |
| author_facet | Guillaume Cardin Cyril Poupet Muriel Bonnet Philippe Veisseire Isabelle Ripoche Pierre Chalard Anne Chauder Etienne Saunier Julien Priam Stéphanie Bornes Laurent Rios |
| author_sort | Guillaume Cardin |
| collection | DOAJ |
| description | Many studies have highlighted the relationship between food and health status, with the aim of improving both disease prevention and life expectancy. Among the different food groups, fermented foods a have huge microbial biodiversity, making them an interesting source of metabolites that could exhibit health benefits. Our previous study highlighted the capacity of raw goat milk cheese, and some of the extracts recovered by the means of chemical fractionation, to increase the longevity of the nematode <i>Caenorhabditis elegans</i>. In this article, we pursued the investigation with a view toward understanding the biological mechanisms involved in this phenomenon. Using mutant nematode strains, we evaluated the implication of the insulin-like DAF-2/DAF-16 and the p38 MAPK pathways in the phenomenon of increased longevity and oxidative-stress resistance mechanisms. Our results demonstrated that freeze-dried raw goat milk cheese, and its extracts, induced the activation of the DAF-2/DAF-16 pathway, increasing longevity. Concerning oxidative-stress resistance, all the extracts increased the survival of the worms, but no evidence of the implication of both of the pathways was highlighted, except for the cheese-lipid extract that did seem to require both pathways to improve the survival rate. Simultaneously, the cheese-lipid extract and the dried extract W70, obtained with water, were able to reduce the reactive oxygen species (ROS) production in human leukocytes. This result is in good correlation with the results obtained with the nematode. |
| first_indexed | 2024-03-10T13:22:23Z |
| format | Article |
| id | doaj.art-81ff76db4de94c1799f2a59d3a178901 |
| institution | Directory Open Access Journal |
| issn | 2072-6643 |
| language | English |
| last_indexed | 2024-03-10T13:22:23Z |
| publishDate | 2021-03-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Nutrients |
| spelling | doaj.art-81ff76db4de94c1799f2a59d3a1789012023-11-21T09:55:08ZengMDPI AGNutrients2072-66432021-03-0113389710.3390/nu13030897A Mechanistic Study of the Antiaging Effect of Raw-Milk Cheese ExtractsGuillaume Cardin0Cyril Poupet1Muriel Bonnet2Philippe Veisseire3Isabelle Ripoche4Pierre Chalard5Anne Chauder6Etienne Saunier7Julien Priam8Stéphanie Bornes9Laurent Rios10Université Clermont Auvergne, INRAE, VetAgro Sup, UMRF, F-15000 Aurillac, FranceUniversité Clermont Auvergne, INRAE, VetAgro Sup, UMRF, F-15000 Aurillac, FranceUniversité Clermont Auvergne, INRAE, VetAgro Sup, UMRF, F-15000 Aurillac, FranceUniversité Clermont Auvergne, INRAE, VetAgro Sup, UMRF, F-15000 Aurillac, FranceUniversité Clermont Auvergne, CNRS, Clermont Auvergne INP, ICCF, F-63000 Clermont-Ferrand, FranceUniversité Clermont Auvergne, CNRS, Clermont Auvergne INP, ICCF, F-63000 Clermont-Ferrand, FranceDômes Pharma, ZAC de Champ Lamet, 3 Rue Andrée Citroën, F-63284 Pont-du-Château, FranceDômes Pharma, ZAC de Champ Lamet, 3 Rue Andrée Citroën, F-63284 Pont-du-Château, FranceDômes Pharma, ZAC de Champ Lamet, 3 Rue Andrée Citroën, F-63284 Pont-du-Château, FranceUniversité Clermont Auvergne, INRAE, VetAgro Sup, UMRF, F-15000 Aurillac, FranceUniversité Clermont Auvergne, INRAE, VetAgro Sup, UMRF, F-15000 Aurillac, FranceMany studies have highlighted the relationship between food and health status, with the aim of improving both disease prevention and life expectancy. Among the different food groups, fermented foods a have huge microbial biodiversity, making them an interesting source of metabolites that could exhibit health benefits. Our previous study highlighted the capacity of raw goat milk cheese, and some of the extracts recovered by the means of chemical fractionation, to increase the longevity of the nematode <i>Caenorhabditis elegans</i>. In this article, we pursued the investigation with a view toward understanding the biological mechanisms involved in this phenomenon. Using mutant nematode strains, we evaluated the implication of the insulin-like DAF-2/DAF-16 and the p38 MAPK pathways in the phenomenon of increased longevity and oxidative-stress resistance mechanisms. Our results demonstrated that freeze-dried raw goat milk cheese, and its extracts, induced the activation of the DAF-2/DAF-16 pathway, increasing longevity. Concerning oxidative-stress resistance, all the extracts increased the survival of the worms, but no evidence of the implication of both of the pathways was highlighted, except for the cheese-lipid extract that did seem to require both pathways to improve the survival rate. Simultaneously, the cheese-lipid extract and the dried extract W70, obtained with water, were able to reduce the reactive oxygen species (ROS) production in human leukocytes. This result is in good correlation with the results obtained with the nematode.https://www.mdpi.com/2072-6643/13/3/897raw-milk cheese<i>Caenorhabditis elegans</i>longevityoxidative stressDAF-16p38 MAPK |
| spellingShingle | Guillaume Cardin Cyril Poupet Muriel Bonnet Philippe Veisseire Isabelle Ripoche Pierre Chalard Anne Chauder Etienne Saunier Julien Priam Stéphanie Bornes Laurent Rios A Mechanistic Study of the Antiaging Effect of Raw-Milk Cheese Extracts Nutrients raw-milk cheese <i>Caenorhabditis elegans</i> longevity oxidative stress DAF-16 p38 MAPK |
| title | A Mechanistic Study of the Antiaging Effect of Raw-Milk Cheese Extracts |
| title_full | A Mechanistic Study of the Antiaging Effect of Raw-Milk Cheese Extracts |
| title_fullStr | A Mechanistic Study of the Antiaging Effect of Raw-Milk Cheese Extracts |
| title_full_unstemmed | A Mechanistic Study of the Antiaging Effect of Raw-Milk Cheese Extracts |
| title_short | A Mechanistic Study of the Antiaging Effect of Raw-Milk Cheese Extracts |
| title_sort | mechanistic study of the antiaging effect of raw milk cheese extracts |
| topic | raw-milk cheese <i>Caenorhabditis elegans</i> longevity oxidative stress DAF-16 p38 MAPK |
| url | https://www.mdpi.com/2072-6643/13/3/897 |
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