3D Bioprinting Human‐Induced Pluripotent Stem Cells and Drug‐Releasing Microspheres to Produce Responsive Neural Tissues
3D bioprinting can produce complex human tissue mimics using stem cells (SCs). Herein, cylindrical constructs containing human‐induced pluripotent stem cell (hiPSC)‐derived neural progenitor cells (NPCs) encapsulated in a fibrin‐based bioink containing polycaprolactone (PCL)–retinoic acid (RA) and p...
| Main Authors: | , , , , , |
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| Format: | Article |
| Language: | English |
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Wiley-VCH
2021-08-01
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| Series: | Advanced NanoBiomed Research |
| Subjects: | |
| Online Access: | https://doi.org/10.1002/anbr.202000077 |
| _version_ | 1819021964528844800 |
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| author | Laura De la Vega Laila Abelseth Ruchi Sharma Juan Triviño-Paredes Milena Restan Stephanie M. Willerth |
| author_facet | Laura De la Vega Laila Abelseth Ruchi Sharma Juan Triviño-Paredes Milena Restan Stephanie M. Willerth |
| author_sort | Laura De la Vega |
| collection | DOAJ |
| description | 3D bioprinting can produce complex human tissue mimics using stem cells (SCs). Herein, cylindrical constructs containing human‐induced pluripotent stem cell (hiPSC)‐derived neural progenitor cells (NPCs) encapsulated in a fibrin‐based bioink containing polycaprolactone (PCL)–retinoic acid (RA) and purmorphamine (puro)‐releasing microspheres are bioprinted in a layer‐by‐layer fashion using the microfluidic‐based RX1 bioprinter to engineer responsive neural tissues. The differentiated constructs contain neurons expressing ChAT, GABA, and MAP2, astrocytes expressing GFAP, and oligodendrocytes expressing O4 as indicated by immunocytochemistry and flow cytometry analysis on days 30 and 45. The bioprinted tissues also respond to treatment with acetylcholine (Ach) and gamma‐aminobutyric acid (GABA) on days 30 and 45. The use of microsphere‐laden bioinks efficiently promotes neural tissue differentiation and maturation in situ using a lower amount of morphogens in comparison with using soluble drugs. This bioprinting strategy serves as a cost‐effective solution for engineering humanized neural tissues. |
| first_indexed | 2024-12-21T04:15:28Z |
| format | Article |
| id | doaj.art-81ffe12472044cce8fbdcbc1b4abf313 |
| institution | Directory Open Access Journal |
| issn | 2699-9307 |
| language | English |
| last_indexed | 2024-12-21T04:15:28Z |
| publishDate | 2021-08-01 |
| publisher | Wiley-VCH |
| record_format | Article |
| series | Advanced NanoBiomed Research |
| spelling | doaj.art-81ffe12472044cce8fbdcbc1b4abf3132022-12-21T19:16:20ZengWiley-VCHAdvanced NanoBiomed Research2699-93072021-08-0118n/an/a10.1002/anbr.2020000773D Bioprinting Human‐Induced Pluripotent Stem Cells and Drug‐Releasing Microspheres to Produce Responsive Neural TissuesLaura De la Vega0Laila Abelseth1Ruchi Sharma2Juan Triviño-Paredes3Milena Restan4Stephanie M. Willerth5Department of Mechanical Engineering University of Victoria Victoria V8W 2Y2 CanadaBiomedical Engineering Program University of Victoria Victoria V8W 2Y2 CanadaDepartment of Mechanical Engineering University of Victoria Victoria V8W 2Y2 CanadaDivision of Medical Sciences University of Victoria Victoria V8W 2Y2 CanadaBiomedical Engineering Program University of Victoria Victoria V8W 2Y2 CanadaDepartment of Mechanical Engineering University of Victoria Victoria V8W 2Y2 Canada3D bioprinting can produce complex human tissue mimics using stem cells (SCs). Herein, cylindrical constructs containing human‐induced pluripotent stem cell (hiPSC)‐derived neural progenitor cells (NPCs) encapsulated in a fibrin‐based bioink containing polycaprolactone (PCL)–retinoic acid (RA) and purmorphamine (puro)‐releasing microspheres are bioprinted in a layer‐by‐layer fashion using the microfluidic‐based RX1 bioprinter to engineer responsive neural tissues. The differentiated constructs contain neurons expressing ChAT, GABA, and MAP2, astrocytes expressing GFAP, and oligodendrocytes expressing O4 as indicated by immunocytochemistry and flow cytometry analysis on days 30 and 45. The bioprinted tissues also respond to treatment with acetylcholine (Ach) and gamma‐aminobutyric acid (GABA) on days 30 and 45. The use of microsphere‐laden bioinks efficiently promotes neural tissue differentiation and maturation in situ using a lower amount of morphogens in comparison with using soluble drugs. This bioprinting strategy serves as a cost‐effective solution for engineering humanized neural tissues.https://doi.org/10.1002/anbr.202000077bioprintingdrug releasingfibrinmicrospheresneural tissuesstem cells |
| spellingShingle | Laura De la Vega Laila Abelseth Ruchi Sharma Juan Triviño-Paredes Milena Restan Stephanie M. Willerth 3D Bioprinting Human‐Induced Pluripotent Stem Cells and Drug‐Releasing Microspheres to Produce Responsive Neural Tissues Advanced NanoBiomed Research bioprinting drug releasing fibrin microspheres neural tissues stem cells |
| title | 3D Bioprinting Human‐Induced Pluripotent Stem Cells and Drug‐Releasing Microspheres to Produce Responsive Neural Tissues |
| title_full | 3D Bioprinting Human‐Induced Pluripotent Stem Cells and Drug‐Releasing Microspheres to Produce Responsive Neural Tissues |
| title_fullStr | 3D Bioprinting Human‐Induced Pluripotent Stem Cells and Drug‐Releasing Microspheres to Produce Responsive Neural Tissues |
| title_full_unstemmed | 3D Bioprinting Human‐Induced Pluripotent Stem Cells and Drug‐Releasing Microspheres to Produce Responsive Neural Tissues |
| title_short | 3D Bioprinting Human‐Induced Pluripotent Stem Cells and Drug‐Releasing Microspheres to Produce Responsive Neural Tissues |
| title_sort | 3d bioprinting human induced pluripotent stem cells and drug releasing microspheres to produce responsive neural tissues |
| topic | bioprinting drug releasing fibrin microspheres neural tissues stem cells |
| url | https://doi.org/10.1002/anbr.202000077 |
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