Morphological and Molecular Changes in the Cortex and Cerebellum of Immunocompetent Mice Infected with Zika Virus
Zika virus (ZIKV) disease continues to be a threat to public health, and it is estimated that millions of people have been infected and that there have been more cases of serious complications than those already reported. Despite many studies on the pathogenesis of ZIKV, several of the genes involve...
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| Language: | English |
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MDPI AG
2023-07-01
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| Series: | Viruses |
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| Online Access: | https://www.mdpi.com/1999-4915/15/8/1632 |
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| author | Aura Caterine Rengifo Jorge Rivera Diego Alejandro Álvarez-Díaz Julián Naizaque Gerardo Santamaria Sheryll Corchuelo Claudia Yadira Gómez Orlando Torres-Fernández |
| author_facet | Aura Caterine Rengifo Jorge Rivera Diego Alejandro Álvarez-Díaz Julián Naizaque Gerardo Santamaria Sheryll Corchuelo Claudia Yadira Gómez Orlando Torres-Fernández |
| author_sort | Aura Caterine Rengifo |
| collection | DOAJ |
| description | Zika virus (ZIKV) disease continues to be a threat to public health, and it is estimated that millions of people have been infected and that there have been more cases of serious complications than those already reported. Despite many studies on the pathogenesis of ZIKV, several of the genes involved in the malformations associated with viral infection are still unknown. In this work, the morphological and molecular changes in the cortex and cerebellum of mice infected with ZIKV were evaluated. Neonatal BALB/c mice were inoculated with ZIKV intraperitoneally, and the respective controls were inoculated with a solution devoid of the virus. At day 10 postinoculation, the mice were euthanized to measure the expression of the markers involved in cortical and cerebellar neurodevelopment. The infected mice presented morphological changes accompanied by calcifications, as well as a decrease in most of the markers evaluated in the cortex and cerebellum. The modifications found could be predictive of astrocytosis, dendritic pathology, alterations in the regulation systems of neuronal excitation and inhibition, and premature maturation, conditions previously described in other models of ZIKV infection and microcephaly. |
| first_indexed | 2024-03-10T23:30:46Z |
| format | Article |
| id | doaj.art-820186ea2a1b48d7b44ce39e6e178afb |
| institution | Directory Open Access Journal |
| issn | 1999-4915 |
| language | English |
| last_indexed | 2024-03-10T23:30:46Z |
| publishDate | 2023-07-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Viruses |
| spelling | doaj.art-820186ea2a1b48d7b44ce39e6e178afb2023-11-19T03:19:28ZengMDPI AGViruses1999-49152023-07-01158163210.3390/v15081632Morphological and Molecular Changes in the Cortex and Cerebellum of Immunocompetent Mice Infected with Zika VirusAura Caterine Rengifo0Jorge Rivera1Diego Alejandro Álvarez-Díaz2Julián Naizaque3Gerardo Santamaria4Sheryll Corchuelo5Claudia Yadira Gómez6Orlando Torres-Fernández7Grupo de Morfología Celular, Dirección de Investigación en Salud Pública, Instituto Nacional de Salud (INS), Avenue 26 No. 51-20–Zone 6 CAN, Bogotá 111321, ColombiaGrupo de Morfología Celular, Dirección de Investigación en Salud Pública, Instituto Nacional de Salud (INS), Avenue 26 No. 51-20–Zone 6 CAN, Bogotá 111321, ColombiaGrupo de Morfología Celular, Dirección de Investigación en Salud Pública, Instituto Nacional de Salud (INS), Avenue 26 No. 51-20–Zone 6 CAN, Bogotá 111321, ColombiaGrupo de Morfología Celular, Dirección de Investigación en Salud Pública, Instituto Nacional de Salud (INS), Avenue 26 No. 51-20–Zone 6 CAN, Bogotá 111321, ColombiaGrupo de Morfología Celular, Dirección de Investigación en Salud Pública, Instituto Nacional de Salud (INS), Avenue 26 No. 51-20–Zone 6 CAN, Bogotá 111321, ColombiaGrupo de Morfología Celular, Dirección de Investigación en Salud Pública, Instituto Nacional de Salud (INS), Avenue 26 No. 51-20–Zone 6 CAN, Bogotá 111321, ColombiaGrupo de Morfología Celular, Dirección de Investigación en Salud Pública, Instituto Nacional de Salud (INS), Avenue 26 No. 51-20–Zone 6 CAN, Bogotá 111321, ColombiaGrupo de Morfología Celular, Dirección de Investigación en Salud Pública, Instituto Nacional de Salud (INS), Avenue 26 No. 51-20–Zone 6 CAN, Bogotá 111321, ColombiaZika virus (ZIKV) disease continues to be a threat to public health, and it is estimated that millions of people have been infected and that there have been more cases of serious complications than those already reported. Despite many studies on the pathogenesis of ZIKV, several of the genes involved in the malformations associated with viral infection are still unknown. In this work, the morphological and molecular changes in the cortex and cerebellum of mice infected with ZIKV were evaluated. Neonatal BALB/c mice were inoculated with ZIKV intraperitoneally, and the respective controls were inoculated with a solution devoid of the virus. At day 10 postinoculation, the mice were euthanized to measure the expression of the markers involved in cortical and cerebellar neurodevelopment. The infected mice presented morphological changes accompanied by calcifications, as well as a decrease in most of the markers evaluated in the cortex and cerebellum. The modifications found could be predictive of astrocytosis, dendritic pathology, alterations in the regulation systems of neuronal excitation and inhibition, and premature maturation, conditions previously described in other models of ZIKV infection and microcephaly.https://www.mdpi.com/1999-4915/15/8/1632neuropathogenesisZika viruscerebral cortexcerebellumneurodevelopmental markers |
| spellingShingle | Aura Caterine Rengifo Jorge Rivera Diego Alejandro Álvarez-Díaz Julián Naizaque Gerardo Santamaria Sheryll Corchuelo Claudia Yadira Gómez Orlando Torres-Fernández Morphological and Molecular Changes in the Cortex and Cerebellum of Immunocompetent Mice Infected with Zika Virus Viruses neuropathogenesis Zika virus cerebral cortex cerebellum neurodevelopmental markers |
| title | Morphological and Molecular Changes in the Cortex and Cerebellum of Immunocompetent Mice Infected with Zika Virus |
| title_full | Morphological and Molecular Changes in the Cortex and Cerebellum of Immunocompetent Mice Infected with Zika Virus |
| title_fullStr | Morphological and Molecular Changes in the Cortex and Cerebellum of Immunocompetent Mice Infected with Zika Virus |
| title_full_unstemmed | Morphological and Molecular Changes in the Cortex and Cerebellum of Immunocompetent Mice Infected with Zika Virus |
| title_short | Morphological and Molecular Changes in the Cortex and Cerebellum of Immunocompetent Mice Infected with Zika Virus |
| title_sort | morphological and molecular changes in the cortex and cerebellum of immunocompetent mice infected with zika virus |
| topic | neuropathogenesis Zika virus cerebral cortex cerebellum neurodevelopmental markers |
| url | https://www.mdpi.com/1999-4915/15/8/1632 |
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