Isolation and Identification of Microorganisms and Antibiotic Resistance Microorganisms from Beehives Located in Palm, Corn and Longan Plantations, Thailand
This study aims to determine the prevalence of microorganisms and antibiotic-resistant microorganisms in beehives located on different plantations in Thailand. Seventeen swabs immersed in transport media were utilized for samples from different zones within beehives. Traditional microbial culture-ba...
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| Format: | Article |
| Language: | English |
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MDPI AG
2023-11-01
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| Series: | Microorganisms |
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| Online Access: | https://www.mdpi.com/2076-2607/11/12/2855 |
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| author | Sirikwan Dokuta Sumed Yadoung Peerapong Jeeno Sayamon Hongjaisee Phadungkiat Khamnoi Sirinya Manochomphu Khanchai Danmek Jakkrawut Maitip Bajaree Chuttong Surat Hongsibsong |
| author_facet | Sirikwan Dokuta Sumed Yadoung Peerapong Jeeno Sayamon Hongjaisee Phadungkiat Khamnoi Sirinya Manochomphu Khanchai Danmek Jakkrawut Maitip Bajaree Chuttong Surat Hongsibsong |
| author_sort | Sirikwan Dokuta |
| collection | DOAJ |
| description | This study aims to determine the prevalence of microorganisms and antibiotic-resistant microorganisms in beehives located on different plantations in Thailand. Seventeen swabs immersed in transport media were utilized for samples from different zones within beehives. Traditional microbial culture-based methods, biochemical tests, MALDI-TOF MS (VITEK<sup>®</sup> MS, bioMerieux, Marcy-l’Étoile, France), and antibiotic drug susceptibility (disk-diffusion) tests were used to detect microorganism and antimicrobial resistance bacteria. The results from 16 beehive swabs found Gram-positive bacteria at 59.5%, Gram-negative bacteria at 35.1%, and fungi (yeast) at 5.4%. These organisms are classified as 11, 11, and 2 types of Gram-positive bacteria, Gram-negative bacteria, and fungi (yeast), respectively. Furthermore, no organism showed resistance to vancomycin or cefoxitin for antibiotic drug susceptibility testing. In contrast, all <i>Acinetobacter</i> spp. were susceptible to ciprofloxacin, levofloxacin, ceftazidime, cefotaxime, imipenem, and meropenem, except for <i>Acinetobacter schindleri</i>, which was resistant to ceftazidime and cefotaxime. For other organisms, due to the limitations of tests to identify some environmental microbial species, the antimicrobial susceptibility test results cannot be interpreted as resistant or susceptible to the drug for these organisms. The study’s findings will support prevention, healthcare services, and public health systems. |
| first_indexed | 2024-03-08T20:32:01Z |
| format | Article |
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| institution | Directory Open Access Journal |
| issn | 2076-2607 |
| language | English |
| last_indexed | 2024-03-08T20:32:01Z |
| publishDate | 2023-11-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Microorganisms |
| spelling | doaj.art-82023c2180fd4bc4bf342b747139298d2023-12-22T14:25:45ZengMDPI AGMicroorganisms2076-26072023-11-011112285510.3390/microorganisms11122855Isolation and Identification of Microorganisms and Antibiotic Resistance Microorganisms from Beehives Located in Palm, Corn and Longan Plantations, ThailandSirikwan Dokuta0Sumed Yadoung1Peerapong Jeeno2Sayamon Hongjaisee3Phadungkiat Khamnoi4Sirinya Manochomphu5Khanchai Danmek6Jakkrawut Maitip7Bajaree Chuttong8Surat Hongsibsong9School of Health Sciences Research, Research Institute for Health Sciences, Chiang Mai University, Chiang Mai 50200, ThailandEnvironmental Sciences Program, Faculty of Sciences, Chiang Mai University, Chiang Mai 50200, ThailandSchool of Health Sciences Research, Research Institute for Health Sciences, Chiang Mai University, Chiang Mai 50200, ThailandSchool of Health Sciences Research, Research Institute for Health Sciences, Chiang Mai University, Chiang Mai 50200, ThailandMicrobiology Unit, Diagnostic Laboratory, Maharaj Nakorn Chiang Mai Hospital, Faculty of Medicine, Chiang Mai University, Chiang Mai 50200, ThailandMicrobiology Unit, Diagnostic Laboratory, Maharaj Nakorn Chiang Mai Hospital, Faculty of Medicine, Chiang Mai University, Chiang Mai 50200, ThailandSchool of Agriculture and Natural Resources, University of Phayao, Phayao 56000, ThailandFaculty of Science, Energy and Environment, Rayong Campus, King Mongkut’s University of Technology North Bangkok, Bankhai 21120, ThailandMeliponini and Apini Research Laboratory, Department of Entomology and Plant Pathology, Faculty of Agriculture, Chiang Mai University, Chiang Mai 50200, ThailandSchool of Health Sciences Research, Research Institute for Health Sciences, Chiang Mai University, Chiang Mai 50200, ThailandThis study aims to determine the prevalence of microorganisms and antibiotic-resistant microorganisms in beehives located on different plantations in Thailand. Seventeen swabs immersed in transport media were utilized for samples from different zones within beehives. Traditional microbial culture-based methods, biochemical tests, MALDI-TOF MS (VITEK<sup>®</sup> MS, bioMerieux, Marcy-l’Étoile, France), and antibiotic drug susceptibility (disk-diffusion) tests were used to detect microorganism and antimicrobial resistance bacteria. The results from 16 beehive swabs found Gram-positive bacteria at 59.5%, Gram-negative bacteria at 35.1%, and fungi (yeast) at 5.4%. These organisms are classified as 11, 11, and 2 types of Gram-positive bacteria, Gram-negative bacteria, and fungi (yeast), respectively. Furthermore, no organism showed resistance to vancomycin or cefoxitin for antibiotic drug susceptibility testing. In contrast, all <i>Acinetobacter</i> spp. were susceptible to ciprofloxacin, levofloxacin, ceftazidime, cefotaxime, imipenem, and meropenem, except for <i>Acinetobacter schindleri</i>, which was resistant to ceftazidime and cefotaxime. For other organisms, due to the limitations of tests to identify some environmental microbial species, the antimicrobial susceptibility test results cannot be interpreted as resistant or susceptible to the drug for these organisms. The study’s findings will support prevention, healthcare services, and public health systems.https://www.mdpi.com/2076-2607/11/12/2855antibiotic resistancemicroorganismsbeehiveplantations in Thailand |
| spellingShingle | Sirikwan Dokuta Sumed Yadoung Peerapong Jeeno Sayamon Hongjaisee Phadungkiat Khamnoi Sirinya Manochomphu Khanchai Danmek Jakkrawut Maitip Bajaree Chuttong Surat Hongsibsong Isolation and Identification of Microorganisms and Antibiotic Resistance Microorganisms from Beehives Located in Palm, Corn and Longan Plantations, Thailand Microorganisms antibiotic resistance microorganisms beehive plantations in Thailand |
| title | Isolation and Identification of Microorganisms and Antibiotic Resistance Microorganisms from Beehives Located in Palm, Corn and Longan Plantations, Thailand |
| title_full | Isolation and Identification of Microorganisms and Antibiotic Resistance Microorganisms from Beehives Located in Palm, Corn and Longan Plantations, Thailand |
| title_fullStr | Isolation and Identification of Microorganisms and Antibiotic Resistance Microorganisms from Beehives Located in Palm, Corn and Longan Plantations, Thailand |
| title_full_unstemmed | Isolation and Identification of Microorganisms and Antibiotic Resistance Microorganisms from Beehives Located in Palm, Corn and Longan Plantations, Thailand |
| title_short | Isolation and Identification of Microorganisms and Antibiotic Resistance Microorganisms from Beehives Located in Palm, Corn and Longan Plantations, Thailand |
| title_sort | isolation and identification of microorganisms and antibiotic resistance microorganisms from beehives located in palm corn and longan plantations thailand |
| topic | antibiotic resistance microorganisms beehive plantations in Thailand |
| url | https://www.mdpi.com/2076-2607/11/12/2855 |
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