Pre-symbiotic response of the compatible host spruce and low-compatibility host pine to the ectomycorrhizal fungus Tricholoma vaccinum
Mutualistic ectomycorrhizal symbiosis requires the exchange of signals even before direct contact of the partners. Volatiles, and specifically volatile terpenoids, can be detected at a distance and may trigger downstream signaling and reprogramming of metabolic responses. The late-stage ectomycorrhi...
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| Format: | Article |
| Language: | English |
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Frontiers Media S.A.
2023-12-01
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| Series: | Frontiers in Microbiology |
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| Online Access: | https://www.frontiersin.org/articles/10.3389/fmicb.2023.1280485/full |
| _version_ | 1797405511045349376 |
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| author | Marycolette Ndidi Ezediokpu Marycolette Ndidi Ezediokpu Rayko Halitschke Katrin Krause Wilhelm Boland Erika Kothe |
| author_facet | Marycolette Ndidi Ezediokpu Marycolette Ndidi Ezediokpu Rayko Halitschke Katrin Krause Wilhelm Boland Erika Kothe |
| author_sort | Marycolette Ndidi Ezediokpu |
| collection | DOAJ |
| description | Mutualistic ectomycorrhizal symbiosis requires the exchange of signals even before direct contact of the partners. Volatiles, and specifically volatile terpenoids, can be detected at a distance and may trigger downstream signaling and reprogramming of metabolic responses. The late-stage ectomycorrhizal fungus Tricholoma vaccinum shows high host specificity with its main host spruce, Picea abies, while rarely associations can be found with pine, Pinus sylvestris. Hence, a comparison of the host and the low-compatibility host’s responses can untangle differences in early signaling during mycorrhiza formation. We investigated sesquiterpenes and identified different patterns of phytohormone responses with spruce and pine. To test the specific role of volatiles, trees were exposed to the complete volatilome of the fungus versus volatiles present when terpene synthases were inhibited by rosuvastatin. The pleiotropic response in spruce included three non-identified products, a pyridine derivative as well as two diterpenes. In pine, other terpenoids responded to the fungal signal. Using exposure to the fungal volatilome with or without terpene synthesis inhibited, we could find a molecular explanation for the longer time needed to establish the low-compatibility interaction. |
| first_indexed | 2024-03-09T03:11:03Z |
| format | Article |
| id | doaj.art-820266f6b78a4bdf817f3645b37abe71 |
| institution | Directory Open Access Journal |
| issn | 1664-302X |
| language | English |
| last_indexed | 2024-03-09T03:11:03Z |
| publishDate | 2023-12-01 |
| publisher | Frontiers Media S.A. |
| record_format | Article |
| series | Frontiers in Microbiology |
| spelling | doaj.art-820266f6b78a4bdf817f3645b37abe712023-12-04T04:31:13ZengFrontiers Media S.A.Frontiers in Microbiology1664-302X2023-12-011410.3389/fmicb.2023.12804851280485Pre-symbiotic response of the compatible host spruce and low-compatibility host pine to the ectomycorrhizal fungus Tricholoma vaccinumMarycolette Ndidi Ezediokpu0Marycolette Ndidi Ezediokpu1Rayko Halitschke2Katrin Krause3Wilhelm Boland4Erika Kothe5Microbial Communication, Institute of Microbiology, Friedrich Schiller University, Jena, GermanyBioorganic Chemistry, Max Planck Institute for Chemical Ecology, Jena, GermanyMass Spectrometry and Metabolomics, Max Planck Institute for Chemical Ecology, Jena, GermanyMicrobial Communication, Institute of Microbiology, Friedrich Schiller University, Jena, GermanyBioorganic Chemistry, Max Planck Institute for Chemical Ecology, Jena, GermanyMicrobial Communication, Institute of Microbiology, Friedrich Schiller University, Jena, GermanyMutualistic ectomycorrhizal symbiosis requires the exchange of signals even before direct contact of the partners. Volatiles, and specifically volatile terpenoids, can be detected at a distance and may trigger downstream signaling and reprogramming of metabolic responses. The late-stage ectomycorrhizal fungus Tricholoma vaccinum shows high host specificity with its main host spruce, Picea abies, while rarely associations can be found with pine, Pinus sylvestris. Hence, a comparison of the host and the low-compatibility host’s responses can untangle differences in early signaling during mycorrhiza formation. We investigated sesquiterpenes and identified different patterns of phytohormone responses with spruce and pine. To test the specific role of volatiles, trees were exposed to the complete volatilome of the fungus versus volatiles present when terpene synthases were inhibited by rosuvastatin. The pleiotropic response in spruce included three non-identified products, a pyridine derivative as well as two diterpenes. In pine, other terpenoids responded to the fungal signal. Using exposure to the fungal volatilome with or without terpene synthesis inhibited, we could find a molecular explanation for the longer time needed to establish the low-compatibility interaction.https://www.frontiersin.org/articles/10.3389/fmicb.2023.1280485/fullectomycorrhizaTricholoma vaccinumfungal volatilomephytohormoneshost tree metabolomePicea abies |
| spellingShingle | Marycolette Ndidi Ezediokpu Marycolette Ndidi Ezediokpu Rayko Halitschke Katrin Krause Wilhelm Boland Erika Kothe Pre-symbiotic response of the compatible host spruce and low-compatibility host pine to the ectomycorrhizal fungus Tricholoma vaccinum Frontiers in Microbiology ectomycorrhiza Tricholoma vaccinum fungal volatilome phytohormones host tree metabolome Picea abies |
| title | Pre-symbiotic response of the compatible host spruce and low-compatibility host pine to the ectomycorrhizal fungus Tricholoma vaccinum |
| title_full | Pre-symbiotic response of the compatible host spruce and low-compatibility host pine to the ectomycorrhizal fungus Tricholoma vaccinum |
| title_fullStr | Pre-symbiotic response of the compatible host spruce and low-compatibility host pine to the ectomycorrhizal fungus Tricholoma vaccinum |
| title_full_unstemmed | Pre-symbiotic response of the compatible host spruce and low-compatibility host pine to the ectomycorrhizal fungus Tricholoma vaccinum |
| title_short | Pre-symbiotic response of the compatible host spruce and low-compatibility host pine to the ectomycorrhizal fungus Tricholoma vaccinum |
| title_sort | pre symbiotic response of the compatible host spruce and low compatibility host pine to the ectomycorrhizal fungus tricholoma vaccinum |
| topic | ectomycorrhiza Tricholoma vaccinum fungal volatilome phytohormones host tree metabolome Picea abies |
| url | https://www.frontiersin.org/articles/10.3389/fmicb.2023.1280485/full |
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