In vitro protective and anti-inflammatory effects of Capparis spinosa and its flavonoids profile
Multiple beneficial effects have been reported to numerous species of Capparis genus. Among these, Capparis spinosa has exhibited several biological benefits, including anti-inflammatory hepatoprotective, antioxidant, and antidiabetic effects. Herein, C. spinosa was extracted with different solvents...
| Main Authors: | , , , , , , , , , |
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| Format: | Article |
| Language: | English |
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De Gruyter
2023-12-01
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| Series: | Open Chemistry |
| Subjects: | |
| Online Access: | https://doi.org/10.1515/chem-2023-0186 |
| _version_ | 1797354366204641280 |
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| author | Alqahtani Ali S. Nasr Fahd A. Ahmed Mohammad Z. Bin Mansour Muath Y. Biksmawi Abdullah A. Noman Omar M. Herqash Rashed N. Al-zharani Mohammed Qurtam Ashraf Ahmed Rudayni Hassan A. |
| author_facet | Alqahtani Ali S. Nasr Fahd A. Ahmed Mohammad Z. Bin Mansour Muath Y. Biksmawi Abdullah A. Noman Omar M. Herqash Rashed N. Al-zharani Mohammed Qurtam Ashraf Ahmed Rudayni Hassan A. |
| author_sort | Alqahtani Ali S. |
| collection | DOAJ |
| description | Multiple beneficial effects have been reported to numerous species of Capparis genus. Among these, Capparis spinosa has exhibited several biological benefits, including anti-inflammatory hepatoprotective, antioxidant, and antidiabetic effects. Herein, C. spinosa was extracted with different solvents and the ability of these fractions to suppress nitric oxide (NO) production in RAW 264.7 cells were assessed via Griess reagent. The effects of C. spinosa fractions on different inflammatory markers were also determined in THP-1 and Human umbilical vein endothelial cells (HUVEC) using reverse transcription polymerase chain reaction. Additionally, high-performance liquid chromatography (HPLC) ultra-violet was employed to estimate the presence of three flavonoid compounds, namely, apigenin, kaempferol, and rutin. Our results indicate that chloroform (CHCl3) and ethyl acetate (EtOAc) fractions of C. spinosa exhibited a promising anti-inflammatory activity via in vitro inhibition of NO in lipopolysaccharide stimulated RAW 264.7 cells. Pretreated stimulated THP-1 cells with either CHCl3 or EtOAc fractions showed decreased expression of interleukin 1 beta (IL-1β) and tumor necrosis factor Alpha in a dose-dependent manner. In HUVEC cells, both fractions downregulate the expression of IL-1β, upregulate the peroxisome proliferator-activated receptor alpha PPAR-α while no significant impact was observed on PPAR-ϒ gene expression. The presence of apigenin, kaempferol, and rutin in the crude extract has been confirmed through HPLC method. Collectively, these results support the ethnopharmacological usage of C. spinosa as a potential therapy for inflammation related conditions including atherosclerosis. |
| first_indexed | 2024-03-08T13:49:23Z |
| format | Article |
| id | doaj.art-820d9a115333412f89a668e1f7ddffbc |
| institution | Directory Open Access Journal |
| issn | 2391-5420 |
| language | English |
| last_indexed | 2024-03-08T13:49:23Z |
| publishDate | 2023-12-01 |
| publisher | De Gruyter |
| record_format | Article |
| series | Open Chemistry |
| spelling | doaj.art-820d9a115333412f89a668e1f7ddffbc2024-01-16T07:18:03ZengDe GruyterOpen Chemistry2391-54202023-12-0121172041810.1515/chem-2023-0186In vitro protective and anti-inflammatory effects of Capparis spinosa and its flavonoids profileAlqahtani Ali S.0Nasr Fahd A.1Ahmed Mohammad Z.2Bin Mansour Muath Y.3Biksmawi Abdullah A.4Noman Omar M.5Herqash Rashed N.6Al-zharani Mohammed7Qurtam Ashraf Ahmed8Rudayni Hassan A.9Department of Pharmacognosy, College of Pharmacy, King Saud University, Riyadh 11451, Saudi ArabiaBiology Department, College of Science, Imam Mohammad Ibn Saud Islamic University (IMSIU), Riyadh 11623, Saudi ArabiaDepartment of Pharmacognosy, College of Pharmacy, King Saud University, Riyadh 11451, Saudi ArabiaDepartment of Pharmacognosy, College of Pharmacy, King Saud University, Riyadh 11451, Saudi ArabiaDepartment of Pharmacognosy, College of Pharmacy, King Saud University, Riyadh 11451, Saudi ArabiaDepartment of Pharmacognosy, College of Pharmacy, King Saud University, Riyadh 11451, Saudi ArabiaDepartment of Pharmacognosy, College of Pharmacy, King Saud University, Riyadh 11451, Saudi ArabiaBiology Department, College of Science, Imam Mohammad Ibn Saud Islamic University (IMSIU), Riyadh 11623, Saudi ArabiaBiology Department, College of Science, Imam Mohammad Ibn Saud Islamic University (IMSIU), Riyadh 11623, Saudi ArabiaBiology Department, College of Science, Imam Mohammad Ibn Saud Islamic University (IMSIU), Riyadh 11623, Saudi ArabiaMultiple beneficial effects have been reported to numerous species of Capparis genus. Among these, Capparis spinosa has exhibited several biological benefits, including anti-inflammatory hepatoprotective, antioxidant, and antidiabetic effects. Herein, C. spinosa was extracted with different solvents and the ability of these fractions to suppress nitric oxide (NO) production in RAW 264.7 cells were assessed via Griess reagent. The effects of C. spinosa fractions on different inflammatory markers were also determined in THP-1 and Human umbilical vein endothelial cells (HUVEC) using reverse transcription polymerase chain reaction. Additionally, high-performance liquid chromatography (HPLC) ultra-violet was employed to estimate the presence of three flavonoid compounds, namely, apigenin, kaempferol, and rutin. Our results indicate that chloroform (CHCl3) and ethyl acetate (EtOAc) fractions of C. spinosa exhibited a promising anti-inflammatory activity via in vitro inhibition of NO in lipopolysaccharide stimulated RAW 264.7 cells. Pretreated stimulated THP-1 cells with either CHCl3 or EtOAc fractions showed decreased expression of interleukin 1 beta (IL-1β) and tumor necrosis factor Alpha in a dose-dependent manner. In HUVEC cells, both fractions downregulate the expression of IL-1β, upregulate the peroxisome proliferator-activated receptor alpha PPAR-α while no significant impact was observed on PPAR-ϒ gene expression. The presence of apigenin, kaempferol, and rutin in the crude extract has been confirmed through HPLC method. Collectively, these results support the ethnopharmacological usage of C. spinosa as a potential therapy for inflammation related conditions including atherosclerosis.https://doi.org/10.1515/chem-2023-0186anti-inflammatorycapparis spinosanitric oxidehuvec |
| spellingShingle | Alqahtani Ali S. Nasr Fahd A. Ahmed Mohammad Z. Bin Mansour Muath Y. Biksmawi Abdullah A. Noman Omar M. Herqash Rashed N. Al-zharani Mohammed Qurtam Ashraf Ahmed Rudayni Hassan A. In vitro protective and anti-inflammatory effects of Capparis spinosa and its flavonoids profile Open Chemistry anti-inflammatory capparis spinosa nitric oxide huvec |
| title | In vitro protective and anti-inflammatory effects of Capparis spinosa and its flavonoids profile |
| title_full | In vitro protective and anti-inflammatory effects of Capparis spinosa and its flavonoids profile |
| title_fullStr | In vitro protective and anti-inflammatory effects of Capparis spinosa and its flavonoids profile |
| title_full_unstemmed | In vitro protective and anti-inflammatory effects of Capparis spinosa and its flavonoids profile |
| title_short | In vitro protective and anti-inflammatory effects of Capparis spinosa and its flavonoids profile |
| title_sort | in vitro protective and anti inflammatory effects of capparis spinosa and its flavonoids profile |
| topic | anti-inflammatory capparis spinosa nitric oxide huvec |
| url | https://doi.org/10.1515/chem-2023-0186 |
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