The determination of benzalkonium chloride in eye drops
ABSTRACT Aim: A novel sensitive kinetic photometric method for the benzalkonium chloride determination has been developed. Materials and method: The method is based on the ability to inhibit the reaction of acetylcholine hydrolysis by cholinesterase. The reaction rate is evaluated by the non-hydro...
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| Format: | Article |
| Language: | English |
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Facultad de Farmacia, Universidad de Granada
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| Series: | Ars Pharmaceutica |
| Subjects: | |
| Online Access: | http://scielo.isciii.es/scielo.php?script=sci_arttext&pid=S2340-98942019000200079&tlng=en |
| _version_ | 1819102180897980416 |
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| author | Blazheyevskiy Mykola Koval’ska Olena |
| author_facet | Blazheyevskiy Mykola Koval’ska Olena |
| author_sort | Blazheyevskiy Mykola |
| collection | DOAJ |
| description | ABSTRACT Aim: A novel sensitive kinetic photometric method for the benzalkonium chloride determination has been developed. Materials and method: The method is based on the ability to inhibit the reaction of acetylcholine hydrolysis by cholinesterase. The reaction rate is evaluated by the non-hydrolysed acetylcholine residue, which is determined by the amount of Peracetic acid, produced during the interaction with the excess of solution hydrogen peroxide . Indicator reaction is an interaction of p-phenetidine with Peracetic acid that leads to the formation of 4,4’-azoxyphenetole with λmax = 358 nm (log10 ε =4.2). Results: The conditions affecting the reaction (reagents concentration, pH, order of addition of reagents, stability in time) have been optimized. The linear dependence has been obeyed in the range of (1.4 to 8.4)·10-6 of benzalkonium chloride with correlation coefficient of 0.999. The assay limit of quantitation (20 % of the inhibition degree) has been 1.9·10-6 mol L-1. Conclusion: The proposed method has been successfully applied to the analysis for eye drops and allowed to confirm accuracy and reliability of the results obtained. |
| first_indexed | 2024-12-22T01:30:28Z |
| format | Article |
| id | doaj.art-820e4dcdac3149afbbea3518aa7d5241 |
| institution | Directory Open Access Journal |
| issn | 2340-9894 |
| language | English |
| last_indexed | 2024-12-22T01:30:28Z |
| publisher | Facultad de Farmacia, Universidad de Granada |
| record_format | Article |
| series | Ars Pharmaceutica |
| spelling | doaj.art-820e4dcdac3149afbbea3518aa7d52412022-12-21T18:43:30ZengFacultad de Farmacia, Universidad de GranadaArs Pharmaceutica2340-989410.30827/ars.v60i2.7920The determination of benzalkonium chloride in eye dropsBlazheyevskiy MykolaKoval’ska OlenaABSTRACT Aim: A novel sensitive kinetic photometric method for the benzalkonium chloride determination has been developed. Materials and method: The method is based on the ability to inhibit the reaction of acetylcholine hydrolysis by cholinesterase. The reaction rate is evaluated by the non-hydrolysed acetylcholine residue, which is determined by the amount of Peracetic acid, produced during the interaction with the excess of solution hydrogen peroxide . Indicator reaction is an interaction of p-phenetidine with Peracetic acid that leads to the formation of 4,4’-azoxyphenetole with λmax = 358 nm (log10 ε =4.2). Results: The conditions affecting the reaction (reagents concentration, pH, order of addition of reagents, stability in time) have been optimized. The linear dependence has been obeyed in the range of (1.4 to 8.4)·10-6 of benzalkonium chloride with correlation coefficient of 0.999. The assay limit of quantitation (20 % of the inhibition degree) has been 1.9·10-6 mol L-1. Conclusion: The proposed method has been successfully applied to the analysis for eye drops and allowed to confirm accuracy and reliability of the results obtained.http://scielo.isciii.es/scielo.php?script=sci_arttext&pid=S2340-98942019000200079&tlng=enBenzalkonium chloridecholinesteraseacetylcholine |
| spellingShingle | Blazheyevskiy Mykola Koval’ska Olena The determination of benzalkonium chloride in eye drops Ars Pharmaceutica Benzalkonium chloride cholinesterase acetylcholine |
| title | The determination of benzalkonium chloride in eye drops |
| title_full | The determination of benzalkonium chloride in eye drops |
| title_fullStr | The determination of benzalkonium chloride in eye drops |
| title_full_unstemmed | The determination of benzalkonium chloride in eye drops |
| title_short | The determination of benzalkonium chloride in eye drops |
| title_sort | determination of benzalkonium chloride in eye drops |
| topic | Benzalkonium chloride cholinesterase acetylcholine |
| url | http://scielo.isciii.es/scielo.php?script=sci_arttext&pid=S2340-98942019000200079&tlng=en |
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