Understanding miRNA Based Gene Regulation in Parkinson’s Disease: An in silico Approach
Parkinson’s disease (PD) is the second most common neurodegenerative disorder, mainly characterized by depletion or insufficient release of dopaminergic neurons in substantia nigra of the midbrain. Literature studies revealed the role of some protein coding genes such as LRRK2, SNCA, DJ-1, and Parki...
| Main Authors: | , |
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| Format: | Article |
| Language: | English |
| Published: |
Bulgarian Academy of Sciences
2020-03-01
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| Series: | International Journal Bioautomation |
| Subjects: | |
| Online Access: | http://www.biomed.bas.bg/bioautomation/2020/vol_24.1/files/24.1_02.pdf |
| Summary: | Parkinson’s disease (PD) is the second most common neurodegenerative disorder, mainly characterized by depletion or insufficient release of dopaminergic neurons in substantia nigra of the midbrain. Literature studies revealed the role of some protein coding genes such as LRRK2, SNCA, DJ-1, and Parkin in the disease pathway of PD and are regulated by few micro RNAs (miRNAs). miRNAs are highly conserved non-coding single stranded RNAs (18-22bp) that target mRNA at 3’UTR (un-translated region) of protein coding genes and act as natural inhibitors. In spite of many researches, miRNAs based gene regulation in PD is still less understood. Therefore, the networks of miRNAs involved in normal development and survival of distinct neuronal populations, which are vulnerable in PD need to be addressed. Argonaute (AGO) protein is a family of protein, which assists miRNAs to bind with mRNAs of the target genes. The current study was undergone to elucidate the binding mechanism between AGO protein and miRNAs, and also with miRNAs-mRNAs duplex at atomic level by implicating computational approach. Therefore, thirty one miRNAs and twenty three different target genes involved in PD pathology were selected from public database and literatures. In silico analysis predicted strong binding affinity between three miRNAs such as miR-27b, miR-124-3p, and miR-29a with mRNAs of CYP1B1 and CDC42 genes respectively which may be considered as potent factors in gene regulation. The current investigation throws light towards understanding miRNAs based gene silencing mechanism in PD. |
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| ISSN: | 1314-1902 1314-2321 |