Microbial-Resistant Salmonella enteritidis Isolated from Poultry Samples

Background: Multidrug resistance in Salmonella enteritidis isolates is a public health problem worldwide; the present study, therefore, was designed for antimicrobial-resistance determination in this strain. Methods: Salmonella strains isolated from poultry samples by biochemical positive and neg...

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Main Authors: Saeedeh Ghazaey, Mohammad Hossain Mirmomeni
Format: Article
Language:English
Published: Varastegan Institute for Medical Sciences 2012-10-01
Series:Reports of Biochemistry and Molecular Biology
Subjects:
Online Access:http://rbmb.net/article-1-22-en.pdf
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author Saeedeh Ghazaey
Mohammad Hossain Mirmomeni
author_facet Saeedeh Ghazaey
Mohammad Hossain Mirmomeni
author_sort Saeedeh Ghazaey
collection DOAJ
description Background: Multidrug resistance in Salmonella enteritidis isolates is a public health problem worldwide; the present study, therefore, was designed for antimicrobial-resistance determination in this strain. Methods: Salmonella strains isolated from poultry samples by biochemical positive and negative tests were subjected to PCR and identified as Salmonella enteritidis. For detection and identification of Salmonella enteritidis isolates, sdfI gene-specific primers were used. Results: We found that 100% of isolates were resistant to ampicillin, 90% were resistant to cephalothin and streptomycin, 70% were resistant to cefotaxime, and 60% were resistant to kanamycin and gentamicin. Conclusion: Salmonella enteritidis isolates had antimicrobial resistance to mentioned antibiotics.
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spelling doaj.art-8212b24aaafc46db9c9b5ba4ac26cf142022-12-21T23:49:25ZengVarastegan Institute for Medical SciencesReports of Biochemistry and Molecular Biology2322-34802322-34802012-10-0111913Microbial-Resistant Salmonella enteritidis Isolated from Poultry SamplesSaeedeh Ghazaey0Mohammad Hossain Mirmomeni1Molecular pathology research center, Ghaem educational and research center, Mashhad university of medical science, Mashhad, Iran.Department of Biology, Faculty of sciences, Razi University, Kermanshah, IranBackground: Multidrug resistance in Salmonella enteritidis isolates is a public health problem worldwide; the present study, therefore, was designed for antimicrobial-resistance determination in this strain. Methods: Salmonella strains isolated from poultry samples by biochemical positive and negative tests were subjected to PCR and identified as Salmonella enteritidis. For detection and identification of Salmonella enteritidis isolates, sdfI gene-specific primers were used. Results: We found that 100% of isolates were resistant to ampicillin, 90% were resistant to cephalothin and streptomycin, 70% were resistant to cefotaxime, and 60% were resistant to kanamycin and gentamicin. Conclusion: Salmonella enteritidis isolates had antimicrobial resistance to mentioned antibiotics.http://rbmb.net/article-1-22-en.pdfAntibiotic ResistancePCRPoultrySalmonella enteritidis
spellingShingle Saeedeh Ghazaey
Mohammad Hossain Mirmomeni
Microbial-Resistant Salmonella enteritidis Isolated from Poultry Samples
Reports of Biochemistry and Molecular Biology
Antibiotic Resistance
PCR
Poultry
Salmonella enteritidis
title Microbial-Resistant Salmonella enteritidis Isolated from Poultry Samples
title_full Microbial-Resistant Salmonella enteritidis Isolated from Poultry Samples
title_fullStr Microbial-Resistant Salmonella enteritidis Isolated from Poultry Samples
title_full_unstemmed Microbial-Resistant Salmonella enteritidis Isolated from Poultry Samples
title_short Microbial-Resistant Salmonella enteritidis Isolated from Poultry Samples
title_sort microbial resistant salmonella enteritidis isolated from poultry samples
topic Antibiotic Resistance
PCR
Poultry
Salmonella enteritidis
url http://rbmb.net/article-1-22-en.pdf
work_keys_str_mv AT saeedehghazaey microbialresistantsalmonellaenteritidisisolatedfrompoultrysamples
AT mohammadhossainmirmomeni microbialresistantsalmonellaenteritidisisolatedfrompoultrysamples