7- DIAGNOSTIC VALUE OF ANTIBODIES TO CITRULLINE CONTAINING PEPTIDES IN PATIENTS WITH RHEUMATOID ARTHRITIS IN SOUTHERN IRAQ43
A serological study has been done in Al-Nassiriyha, southern Iraq, from December 2006 to August 2007 designed to determine the frequency of anti-cyclic citrullinated peptide antibody (anti-CCP) in a group of patients with rheumatoid arthritis for specific period of time compared with other rheumatic...
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| Format: | Article |
| Language: | English |
| Published: |
university of basrah
2009-06-01
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| Series: | Basrah Journal of Surgery |
| Subjects: | |
| Online Access: | https://bjsrg.uobasrah.edu.iq/article_55269_8df6f0d0965cd639b843f497215fc2e1.pdf |
| _version_ | 1823941854016569344 |
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| author | THURAYA J MEZAL ADNAN T AL-KHAFAJI HASSAN J HASONY ALI M BARAKAT |
| author_facet | THURAYA J MEZAL ADNAN T AL-KHAFAJI HASSAN J HASONY ALI M BARAKAT |
| author_sort | THURAYA J MEZAL |
| collection | DOAJ |
| description | A serological study has been done in Al-Nassiriyha, southern Iraq, from December 2006 to
August 2007 designed to determine the frequency of anti-cyclic citrullinated peptide antibody
(anti-CCP) in a group of patients with rheumatoid arthritis for specific period of time compared
with other rheumatic diseases and healthy controls. Also to show the diagnostic value of
anti-CCP antibody in relation to other markers [rheumatoid factor (RF), antinuclear factor (ANF),
C-reactive protein (CRP) and cytokines such as interleukin–6 (IL-6), tumor necrosis factor–α
(TNF-α)] to discriminate between those patients with and without RA.
Blood samples were taken from 121 patients with rheumatoid arthritis, 88 patients of other
rheumatic diseases from AL-Hussein Teaching Hospital in AL-Nassiriyah and 120 healthy
controls from medical personnel and school students who were healthy. All the information was
taken from the patients and controls.
An Enzyme-linked immunosorbent assay was used for estimation of anti-CCP, IL-6, TNF-α and
slide agglutination test for the estimation of RF, CRP and ANF. The seropositivity to anti-CCP in
RA patients group was 61.2% among them 23% with low levels of anti-CCP, 25% with
moderate levels and 51.3% with high levels (> 60 units /ml.).The high levels of anti-CCP persist
for long durations up to 10 years (54.7%). However, anti-CCP was rarely detected among non-
RA rheumatic diseases and healthy controls (2.3% and 2.5% respectively). Anti-CCP showed
the greater specificity (97.6%) and sensitivity (61.2%) compared to the other marker used in this
study. The overall agreement between the tested parameters in their ability to detect RA patient
was higher for anti-CCP combined to IL-6 (91.7%) in comparison to the effect of other markers
combination. Although anti-CCP was more common in RA patient with 2-10 years duration of
illness, anti-CCP, IL-6 and TNF-α positivity as estimated by ELISA were persistently with high
titers regardless the duration of illness. The majority of RA patients with high anti-CCP levels
were among stage three of clinical RA. Smoking has a positive relation to the increased levels
of all tested parameters compared to the non-smokers. |
| first_indexed | 2024-12-17T04:32:02Z |
| format | Article |
| id | doaj.art-8213a8d58092476bb2ebc7aa18c4bf26 |
| institution | Directory Open Access Journal |
| issn | 1683-3589 2409-501X |
| language | English |
| last_indexed | 2024-12-17T04:32:02Z |
| publishDate | 2009-06-01 |
| publisher | university of basrah |
| record_format | Article |
| series | Basrah Journal of Surgery |
| spelling | doaj.art-8213a8d58092476bb2ebc7aa18c4bf262022-12-21T22:03:29Zenguniversity of basrahBasrah Journal of Surgery1683-35892409-501X2009-06-01151435010.33762/bsurg.2009.55269552697- DIAGNOSTIC VALUE OF ANTIBODIES TO CITRULLINE CONTAINING PEPTIDES IN PATIENTS WITH RHEUMATOID ARTHRITIS IN SOUTHERN IRAQ43THURAYA J MEZALADNAN T AL-KHAFAJIHASSAN J HASONYALI M BARAKATA serological study has been done in Al-Nassiriyha, southern Iraq, from December 2006 to August 2007 designed to determine the frequency of anti-cyclic citrullinated peptide antibody (anti-CCP) in a group of patients with rheumatoid arthritis for specific period of time compared with other rheumatic diseases and healthy controls. Also to show the diagnostic value of anti-CCP antibody in relation to other markers [rheumatoid factor (RF), antinuclear factor (ANF), C-reactive protein (CRP) and cytokines such as interleukin–6 (IL-6), tumor necrosis factor–α (TNF-α)] to discriminate between those patients with and without RA. Blood samples were taken from 121 patients with rheumatoid arthritis, 88 patients of other rheumatic diseases from AL-Hussein Teaching Hospital in AL-Nassiriyah and 120 healthy controls from medical personnel and school students who were healthy. All the information was taken from the patients and controls. An Enzyme-linked immunosorbent assay was used for estimation of anti-CCP, IL-6, TNF-α and slide agglutination test for the estimation of RF, CRP and ANF. The seropositivity to anti-CCP in RA patients group was 61.2% among them 23% with low levels of anti-CCP, 25% with moderate levels and 51.3% with high levels (> 60 units /ml.).The high levels of anti-CCP persist for long durations up to 10 years (54.7%). However, anti-CCP was rarely detected among non- RA rheumatic diseases and healthy controls (2.3% and 2.5% respectively). Anti-CCP showed the greater specificity (97.6%) and sensitivity (61.2%) compared to the other marker used in this study. The overall agreement between the tested parameters in their ability to detect RA patient was higher for anti-CCP combined to IL-6 (91.7%) in comparison to the effect of other markers combination. Although anti-CCP was more common in RA patient with 2-10 years duration of illness, anti-CCP, IL-6 and TNF-α positivity as estimated by ELISA were persistently with high titers regardless the duration of illness. The majority of RA patients with high anti-CCP levels were among stage three of clinical RA. Smoking has a positive relation to the increased levels of all tested parameters compared to the non-smokers.https://bjsrg.uobasrah.edu.iq/article_55269_8df6f0d0965cd639b843f497215fc2e1.pdfrheumatoidarthritis |
| spellingShingle | THURAYA J MEZAL ADNAN T AL-KHAFAJI HASSAN J HASONY ALI M BARAKAT 7- DIAGNOSTIC VALUE OF ANTIBODIES TO CITRULLINE CONTAINING PEPTIDES IN PATIENTS WITH RHEUMATOID ARTHRITIS IN SOUTHERN IRAQ43 Basrah Journal of Surgery rheumatoid arthritis |
| title | 7- DIAGNOSTIC VALUE OF ANTIBODIES TO CITRULLINE CONTAINING PEPTIDES IN PATIENTS WITH RHEUMATOID ARTHRITIS IN SOUTHERN IRAQ43 |
| title_full | 7- DIAGNOSTIC VALUE OF ANTIBODIES TO CITRULLINE CONTAINING PEPTIDES IN PATIENTS WITH RHEUMATOID ARTHRITIS IN SOUTHERN IRAQ43 |
| title_fullStr | 7- DIAGNOSTIC VALUE OF ANTIBODIES TO CITRULLINE CONTAINING PEPTIDES IN PATIENTS WITH RHEUMATOID ARTHRITIS IN SOUTHERN IRAQ43 |
| title_full_unstemmed | 7- DIAGNOSTIC VALUE OF ANTIBODIES TO CITRULLINE CONTAINING PEPTIDES IN PATIENTS WITH RHEUMATOID ARTHRITIS IN SOUTHERN IRAQ43 |
| title_short | 7- DIAGNOSTIC VALUE OF ANTIBODIES TO CITRULLINE CONTAINING PEPTIDES IN PATIENTS WITH RHEUMATOID ARTHRITIS IN SOUTHERN IRAQ43 |
| title_sort | 7 diagnostic value of antibodies to citrulline containing peptides in patients with rheumatoid arthritis in southern iraq43 |
| topic | rheumatoid arthritis |
| url | https://bjsrg.uobasrah.edu.iq/article_55269_8df6f0d0965cd639b843f497215fc2e1.pdf |
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