Mg-protoporphyrin IX signals enhance plant’s tolerance to cold stress
The relationship between Mg-protoporphyrin IX (Mg-Proto IX) signals and plant’s tolerance to cold stress is investigated. Arabidopsis seedlings grown for 3 weeks were pretreated with 2 mM glutamate and 2 mM MgCl2 for 48 h at room temperature to induce Mg-Proto IX accumulation. Then cold stress was p...
| Main Authors: | , , , , , , , , , |
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| Format: | Article |
| Language: | English |
| Published: |
Frontiers Media S.A.
2016-10-01
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| Series: | Frontiers in Plant Science |
| Subjects: | |
| Online Access: | http://journal.frontiersin.org/Journal/10.3389/fpls.2016.01545/full |
| _version_ | 1819264987206516736 |
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| author | Zhong-Wei Zhang Zi-Li Wu Ling-Yang Feng Li-Hua Dong An-Jun Song Ming Yuan Yang-Er Chen Jian Zeng Guang-Deng Chen Shu Yuan |
| author_facet | Zhong-Wei Zhang Zi-Li Wu Ling-Yang Feng Li-Hua Dong An-Jun Song Ming Yuan Yang-Er Chen Jian Zeng Guang-Deng Chen Shu Yuan |
| author_sort | Zhong-Wei Zhang |
| collection | DOAJ |
| description | The relationship between Mg-protoporphyrin IX (Mg-Proto IX) signals and plant’s tolerance to cold stress is investigated. Arabidopsis seedlings grown for 3 weeks were pretreated with 2 mM glutamate and 2 mM MgCl2 for 48 h at room temperature to induce Mg-Proto IX accumulation. Then cold stress was performed at 4 °C for additional 72 h. Glutamate + MgCl2 pre-treatments alleviated the subsequent cold stress significantly by rising the leaf temperature through inducing Mg-Proto IX signals. The protective role of glutamate + MgCl2 treatment was greatly compromised in the mutants of Mg-Proto IX synthesis, Mg-Proto IX signaling and cyanide-resistant respiration. And the enhancement of cold-responsive gene expression was greatly compromised in the mutants of Mg-Proto IX synthesis, Mg-Proto IX signaling and ABA signaling, but not in the mutant of cyanide-resistant respiration. Cold stress promoted cyanide-resistant respiration and leaf total respiration exponentially, which could be further induced by the glutamate + MgCl2 treatment. Mg-Proto IX signals also activate antioxidant enzymes and increase non-enzymatic antioxidants (glutathione but not ascorbic acid) to maintain redox equilibrium during the cold stress. |
| first_indexed | 2024-12-23T20:38:13Z |
| format | Article |
| id | doaj.art-82153d6bd8dd406a81fd69704e0fa4d0 |
| institution | Directory Open Access Journal |
| issn | 1664-462X |
| language | English |
| last_indexed | 2024-12-23T20:38:13Z |
| publishDate | 2016-10-01 |
| publisher | Frontiers Media S.A. |
| record_format | Article |
| series | Frontiers in Plant Science |
| spelling | doaj.art-82153d6bd8dd406a81fd69704e0fa4d02022-12-21T17:32:01ZengFrontiers Media S.A.Frontiers in Plant Science1664-462X2016-10-01710.3389/fpls.2016.01545197457Mg-protoporphyrin IX signals enhance plant’s tolerance to cold stressZhong-Wei Zhang0Zi-Li Wu1Ling-Yang Feng2Li-Hua Dong3An-Jun Song4Ming Yuan5Yang-Er Chen6Jian Zeng7Guang-Deng Chen8Shu Yuan9Sichuan Agricultural UniversityYibin UniversitySichuan Agricultural UniversitySichuan Agricultural UniversitySichuan Agricultural UniversitySichuan Agricultural UniversitySichuan Agricultural UniversitySichuan Agricultural UniversitySichuan Agricultural UniversitySichuan Agricultural UniversityThe relationship between Mg-protoporphyrin IX (Mg-Proto IX) signals and plant’s tolerance to cold stress is investigated. Arabidopsis seedlings grown for 3 weeks were pretreated with 2 mM glutamate and 2 mM MgCl2 for 48 h at room temperature to induce Mg-Proto IX accumulation. Then cold stress was performed at 4 °C for additional 72 h. Glutamate + MgCl2 pre-treatments alleviated the subsequent cold stress significantly by rising the leaf temperature through inducing Mg-Proto IX signals. The protective role of glutamate + MgCl2 treatment was greatly compromised in the mutants of Mg-Proto IX synthesis, Mg-Proto IX signaling and cyanide-resistant respiration. And the enhancement of cold-responsive gene expression was greatly compromised in the mutants of Mg-Proto IX synthesis, Mg-Proto IX signaling and ABA signaling, but not in the mutant of cyanide-resistant respiration. Cold stress promoted cyanide-resistant respiration and leaf total respiration exponentially, which could be further induced by the glutamate + MgCl2 treatment. Mg-Proto IX signals also activate antioxidant enzymes and increase non-enzymatic antioxidants (glutathione but not ascorbic acid) to maintain redox equilibrium during the cold stress.http://journal.frontiersin.org/Journal/10.3389/fpls.2016.01545/fullGlutathionecold stressCyanide-resistant respirationantioxidant enzymeMg-protoporphyrin IX signals. |
| spellingShingle | Zhong-Wei Zhang Zi-Li Wu Ling-Yang Feng Li-Hua Dong An-Jun Song Ming Yuan Yang-Er Chen Jian Zeng Guang-Deng Chen Shu Yuan Mg-protoporphyrin IX signals enhance plant’s tolerance to cold stress Frontiers in Plant Science Glutathione cold stress Cyanide-resistant respiration antioxidant enzyme Mg-protoporphyrin IX signals. |
| title | Mg-protoporphyrin IX signals enhance plant’s tolerance to cold stress |
| title_full | Mg-protoporphyrin IX signals enhance plant’s tolerance to cold stress |
| title_fullStr | Mg-protoporphyrin IX signals enhance plant’s tolerance to cold stress |
| title_full_unstemmed | Mg-protoporphyrin IX signals enhance plant’s tolerance to cold stress |
| title_short | Mg-protoporphyrin IX signals enhance plant’s tolerance to cold stress |
| title_sort | mg protoporphyrin ix signals enhance plant s tolerance to cold stress |
| topic | Glutathione cold stress Cyanide-resistant respiration antioxidant enzyme Mg-protoporphyrin IX signals. |
| url | http://journal.frontiersin.org/Journal/10.3389/fpls.2016.01545/full |
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