Impact of Light Intensity on Antioxidant Activity of Tropical Microalgae
Twelve microalgae species isolated in tropical lagoons of New Caledonia were screened as a new source of antioxidants. Microalgae were cultivated at two light intensities to investigate their influence on antioxidant capacity. To assess antioxidant property of microalgae extracts, four assays with d...
| Main Authors: | , , , , , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
MDPI AG
2020-02-01
|
| Series: | Marine Drugs |
| Subjects: | |
| Online Access: | https://www.mdpi.com/1660-3397/18/2/122 |
| _version_ | 1828353382234980352 |
|---|---|
| author | Noémie Coulombier Elodie Nicolau Loïc Le Déan Cyril Antheaume Thierry Jauffrais Nicolas Lebouvier |
| author_facet | Noémie Coulombier Elodie Nicolau Loïc Le Déan Cyril Antheaume Thierry Jauffrais Nicolas Lebouvier |
| author_sort | Noémie Coulombier |
| collection | DOAJ |
| description | Twelve microalgae species isolated in tropical lagoons of New Caledonia were screened as a new source of antioxidants. Microalgae were cultivated at two light intensities to investigate their influence on antioxidant capacity. To assess antioxidant property of microalgae extracts, four assays with different modes of action were used: 1,1-diphenyl-2-picrylhydrazyl (DPPH), 2,2’-azino-bis (3-éthylbenzothiazoline-6-sulphonique) (ABTS), oxygen radical absorbance capacity (ORAC), and thiobabituric acid reactive substances (TBARS). This screening was coupled to pigment analysis to link antioxidant activity and carotenoid content. The results showed that none of the microalgae studied can scavenge DPPH and ABTS radicals, but <i>Chaetoceros</i> sp., <i>Nephroselmis</i> sp., and <i>Nitzschia</i> A sp. have the capacity to scavenge peroxyl radical (ORAC) and <i>Tetraselmis</i> sp., <i>Nitzschia</i> A sp., and <i>Nephroselmis</i> sp. can inhibit lipid peroxidation (TBARS). Carotenoid composition is typical of the studied microalgae and highlight the siphonaxanthin, detected in <i>Nephroselmis</i> sp., as a pigment of interest. It was found that xanthophylls were the major contributors to the peroxyl radical scavenging capacity measured with ORAC assay, but there was no link between carotenoids and inhibition of lipid peroxidation measured with TBARS assay. In addition, the results showed that light intensity has a strong influence on antioxidant capacity of microalgae: Overall, antioxidant activities measured with ORAC assay are better in high light intensity whereas antioxidant activities measured with TBARS assay are better in low light intensity. It suggests that different antioxidant compounds production is related to light intensity. |
| first_indexed | 2024-04-14T02:07:01Z |
| format | Article |
| id | doaj.art-8215d5688ba14e81a03b26a1640a729f |
| institution | Directory Open Access Journal |
| issn | 1660-3397 |
| language | English |
| last_indexed | 2024-04-14T02:07:01Z |
| publishDate | 2020-02-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Marine Drugs |
| spelling | doaj.art-8215d5688ba14e81a03b26a1640a729f2022-12-22T02:18:38ZengMDPI AGMarine Drugs1660-33972020-02-0118212210.3390/md18020122md18020122Impact of Light Intensity on Antioxidant Activity of Tropical MicroalgaeNoémie Coulombier0Elodie Nicolau1Loïc Le Déan2Cyril Antheaume3Thierry Jauffrais4Nicolas Lebouvier5ADECAL Technopole, 1 bis rue Berthelot, 98846 Noumea, New CaledoniaIfremer, RBE/BRM/PBA, Rue de l’île d’Yeu, 44311 Nantes, FranceIfremer, UMR 9220 ENTROPIE, RBE/LEAD, 101 Promenade Roger Laroque, 98897 Noumea, New CaledoniaISEA, EA7484, Université de Nouvelle Calédonie, Campus de Nouville, 98851 Nouméa, New CaledoniaIfremer, UMR 9220 ENTROPIE, RBE/LEAD, 101 Promenade Roger Laroque, 98897 Noumea, New CaledoniaISEA, EA7484, Université de Nouvelle Calédonie, Campus de Nouville, 98851 Nouméa, New CaledoniaTwelve microalgae species isolated in tropical lagoons of New Caledonia were screened as a new source of antioxidants. Microalgae were cultivated at two light intensities to investigate their influence on antioxidant capacity. To assess antioxidant property of microalgae extracts, four assays with different modes of action were used: 1,1-diphenyl-2-picrylhydrazyl (DPPH), 2,2’-azino-bis (3-éthylbenzothiazoline-6-sulphonique) (ABTS), oxygen radical absorbance capacity (ORAC), and thiobabituric acid reactive substances (TBARS). This screening was coupled to pigment analysis to link antioxidant activity and carotenoid content. The results showed that none of the microalgae studied can scavenge DPPH and ABTS radicals, but <i>Chaetoceros</i> sp., <i>Nephroselmis</i> sp., and <i>Nitzschia</i> A sp. have the capacity to scavenge peroxyl radical (ORAC) and <i>Tetraselmis</i> sp., <i>Nitzschia</i> A sp., and <i>Nephroselmis</i> sp. can inhibit lipid peroxidation (TBARS). Carotenoid composition is typical of the studied microalgae and highlight the siphonaxanthin, detected in <i>Nephroselmis</i> sp., as a pigment of interest. It was found that xanthophylls were the major contributors to the peroxyl radical scavenging capacity measured with ORAC assay, but there was no link between carotenoids and inhibition of lipid peroxidation measured with TBARS assay. In addition, the results showed that light intensity has a strong influence on antioxidant capacity of microalgae: Overall, antioxidant activities measured with ORAC assay are better in high light intensity whereas antioxidant activities measured with TBARS assay are better in low light intensity. It suggests that different antioxidant compounds production is related to light intensity.https://www.mdpi.com/1660-3397/18/2/122<i>nephroselmis</i>light intensityin vitro antioxidant activitysiphonaxanthincarotenoidbioactive compounds |
| spellingShingle | Noémie Coulombier Elodie Nicolau Loïc Le Déan Cyril Antheaume Thierry Jauffrais Nicolas Lebouvier Impact of Light Intensity on Antioxidant Activity of Tropical Microalgae Marine Drugs <i>nephroselmis</i> light intensity in vitro antioxidant activity siphonaxanthin carotenoid bioactive compounds |
| title | Impact of Light Intensity on Antioxidant Activity of Tropical Microalgae |
| title_full | Impact of Light Intensity on Antioxidant Activity of Tropical Microalgae |
| title_fullStr | Impact of Light Intensity on Antioxidant Activity of Tropical Microalgae |
| title_full_unstemmed | Impact of Light Intensity on Antioxidant Activity of Tropical Microalgae |
| title_short | Impact of Light Intensity on Antioxidant Activity of Tropical Microalgae |
| title_sort | impact of light intensity on antioxidant activity of tropical microalgae |
| topic | <i>nephroselmis</i> light intensity in vitro antioxidant activity siphonaxanthin carotenoid bioactive compounds |
| url | https://www.mdpi.com/1660-3397/18/2/122 |
| work_keys_str_mv | AT noemiecoulombier impactoflightintensityonantioxidantactivityoftropicalmicroalgae AT elodienicolau impactoflightintensityonantioxidantactivityoftropicalmicroalgae AT loicledean impactoflightintensityonantioxidantactivityoftropicalmicroalgae AT cyrilantheaume impactoflightintensityonantioxidantactivityoftropicalmicroalgae AT thierryjauffrais impactoflightintensityonantioxidantactivityoftropicalmicroalgae AT nicolaslebouvier impactoflightintensityonantioxidantactivityoftropicalmicroalgae |