Impact of application of bio-amniotic membrane immersed in 5-fluorouracil solution in trabeculectomy on rabbit retina
Background : To observe the impact of application of bio-amniotic membrane immersed in 5-fluorouracil solution in trabeculectomy on the retina in a rabbit model. Materials and Methods : Healthy white New Zealand rabbits were randomly assigned into three groups with 20 in each group. Bio-amniotic mem...
| Main Authors: | , , , , , , , , , |
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| Format: | Article |
| Language: | English |
| Published: |
Wolters Kluwer Medknow Publications
2013-01-01
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| Series: | Indian Journal of Ophthalmology |
| Subjects: | |
| Online Access: | http://www.ijo.in/article.asp?issn=0301-4738;year=2013;volume=61;issue=12;spage=734;epage=738;aulast=Zhang |
| _version_ | 1818897289729540096 |
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| author | Chenming Zhang Xinyi Wu Wei Liu Jianrong Wang Bailin Guo Yan Sun Yuguang Zhang Hua Zhang Yu Wang Jun Zhao |
| author_facet | Chenming Zhang Xinyi Wu Wei Liu Jianrong Wang Bailin Guo Yan Sun Yuguang Zhang Hua Zhang Yu Wang Jun Zhao |
| author_sort | Chenming Zhang |
| collection | DOAJ |
| description | Background : To observe the impact of application of bio-amniotic membrane immersed in 5-fluorouracil solution in trabeculectomy on the retina in a rabbit model. Materials and Methods : Healthy white New Zealand rabbits were randomly assigned into three groups with 20 in each group. Bio-amniotic membranes of 4 × 5 mm immersed in either physiological saline/water for 10 min, or 25 mg/mL 5-fluorouracil solution for 5 and 10 min, respectively, were applied on rabbit eyes during trabeculectomy. At 7, 14, 21, and 28 days of postoperation, five rabbits from each group were examined with electroretinogram (ERG). After being examined for eye pressure and bleb morphology, rabbits were sacrificed by air embolism and their retinas were collected and examined by transmission electron microscopy (TEM). In addition, 5-fluorouracil amount in bio-amniotic membranes was measured using high-performance liquid chromatography. Results: Each bio-amniotic membrane could absorb 59.004 μg and 75.828 μg 5-fluorouracil after being immersed in 5-fluorouracil solution for 5 and 10 min, respectively. Application of these bio-amniotic membranes in trabeculectomy could promote the formation of well-functioning bleb and maintain intraocular pressure, although it had no effect on retina structures as examined with ERG and TEM. Conclusion: Application of 5-FU soaked bio-amniotic membrane in rabbit eye trabeculectomy is effective and safe. |
| first_indexed | 2024-12-19T19:13:49Z |
| format | Article |
| id | doaj.art-82170ac6527a4fd1b7007bda7850b60c |
| institution | Directory Open Access Journal |
| issn | 0301-4738 1998-3689 |
| language | English |
| last_indexed | 2024-12-19T19:13:49Z |
| publishDate | 2013-01-01 |
| publisher | Wolters Kluwer Medknow Publications |
| record_format | Article |
| series | Indian Journal of Ophthalmology |
| spelling | doaj.art-82170ac6527a4fd1b7007bda7850b60c2022-12-21T20:09:12ZengWolters Kluwer Medknow PublicationsIndian Journal of Ophthalmology0301-47381998-36892013-01-01611273473810.4103/0301-4738.121151Impact of application of bio-amniotic membrane immersed in 5-fluorouracil solution in trabeculectomy on rabbit retinaChenming ZhangXinyi WuWei LiuJianrong WangBailin GuoYan SunYuguang ZhangHua ZhangYu WangJun ZhaoBackground : To observe the impact of application of bio-amniotic membrane immersed in 5-fluorouracil solution in trabeculectomy on the retina in a rabbit model. Materials and Methods : Healthy white New Zealand rabbits were randomly assigned into three groups with 20 in each group. Bio-amniotic membranes of 4 × 5 mm immersed in either physiological saline/water for 10 min, or 25 mg/mL 5-fluorouracil solution for 5 and 10 min, respectively, were applied on rabbit eyes during trabeculectomy. At 7, 14, 21, and 28 days of postoperation, five rabbits from each group were examined with electroretinogram (ERG). After being examined for eye pressure and bleb morphology, rabbits were sacrificed by air embolism and their retinas were collected and examined by transmission electron microscopy (TEM). In addition, 5-fluorouracil amount in bio-amniotic membranes was measured using high-performance liquid chromatography. Results: Each bio-amniotic membrane could absorb 59.004 μg and 75.828 μg 5-fluorouracil after being immersed in 5-fluorouracil solution for 5 and 10 min, respectively. Application of these bio-amniotic membranes in trabeculectomy could promote the formation of well-functioning bleb and maintain intraocular pressure, although it had no effect on retina structures as examined with ERG and TEM. Conclusion: Application of 5-FU soaked bio-amniotic membrane in rabbit eye trabeculectomy is effective and safe.http://www.ijo.in/article.asp?issn=0301-4738;year=2013;volume=61;issue=12;spage=734;epage=738;aulast=ZhangAmblyopianasolacrimal duct obstructionprobingDuane syndromeesotropiastrabismusBevacizumabmitochondrial functionmicrovascular endothelial cellsneurosensory retinal cellsretinal pigment epithelial cellstissue cultureClinical donor tissue gradingdeath to enucleation timedeath to preservation timeeye bank specular microscopyoptimizing donor tissue utilizationDCRendodiathermy bipolar probenasolacrimal duct obstructionrecanalizeAge-related macular degenerationcarotenoidscross-sectional studiesluteinChildrenGDx variable corneal compensationIndiannormalretinal nerve fiber layer thicknessBiological amniotic membrane5-fluorouracilretinatrabeculectomy |
| spellingShingle | Chenming Zhang Xinyi Wu Wei Liu Jianrong Wang Bailin Guo Yan Sun Yuguang Zhang Hua Zhang Yu Wang Jun Zhao Impact of application of bio-amniotic membrane immersed in 5-fluorouracil solution in trabeculectomy on rabbit retina Indian Journal of Ophthalmology Amblyopia nasolacrimal duct obstruction probing Duane syndrome esotropia strabismus Bevacizumab mitochondrial function microvascular endothelial cells neurosensory retinal cells retinal pigment epithelial cells tissue culture Clinical donor tissue grading death to enucleation time death to preservation time eye bank specular microscopy optimizing donor tissue utilization DCR endodiathermy bipolar probe nasolacrimal duct obstruction recanalize Age-related macular degeneration carotenoids cross-sectional studies lutein Children GDx variable corneal compensation Indian normal retinal nerve fiber layer thickness Biological amniotic membrane 5-fluorouracil retina trabeculectomy |
| title | Impact of application of bio-amniotic membrane immersed in 5-fluorouracil solution in trabeculectomy on rabbit retina |
| title_full | Impact of application of bio-amniotic membrane immersed in 5-fluorouracil solution in trabeculectomy on rabbit retina |
| title_fullStr | Impact of application of bio-amniotic membrane immersed in 5-fluorouracil solution in trabeculectomy on rabbit retina |
| title_full_unstemmed | Impact of application of bio-amniotic membrane immersed in 5-fluorouracil solution in trabeculectomy on rabbit retina |
| title_short | Impact of application of bio-amniotic membrane immersed in 5-fluorouracil solution in trabeculectomy on rabbit retina |
| title_sort | impact of application of bio amniotic membrane immersed in 5 fluorouracil solution in trabeculectomy on rabbit retina |
| topic | Amblyopia nasolacrimal duct obstruction probing Duane syndrome esotropia strabismus Bevacizumab mitochondrial function microvascular endothelial cells neurosensory retinal cells retinal pigment epithelial cells tissue culture Clinical donor tissue grading death to enucleation time death to preservation time eye bank specular microscopy optimizing donor tissue utilization DCR endodiathermy bipolar probe nasolacrimal duct obstruction recanalize Age-related macular degeneration carotenoids cross-sectional studies lutein Children GDx variable corneal compensation Indian normal retinal nerve fiber layer thickness Biological amniotic membrane 5-fluorouracil retina trabeculectomy |
| url | http://www.ijo.in/article.asp?issn=0301-4738;year=2013;volume=61;issue=12;spage=734;epage=738;aulast=Zhang |
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