T-cell and serological responses to Erp, an exported <it>Mycobacterium tuberculosis </it>protein, in tuberculosis patients and healthy individuals
<p>Abstract</p> <p>Background</p> <p>The identification of antigens able to differentiate tuberculosis (TB) disease from TB infection would be valuable. Cellular and humoral immune responses to Erp (Exported repetitive protein) – a recently identified <it>M. tuber...
| Main Authors: | , , , , , , , , , , , |
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| Format: | Article |
| Language: | English |
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BMC
2007-07-01
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| Series: | BMC Infectious Diseases |
| Online Access: | http://www.biomedcentral.com/1471-2334/7/83 |
| _version_ | 1818145556672806912 |
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| author | Ottenhoff Tom Arend Sandra M Bricaire François Truffot Chantal Sellier Pierre Mauger Isabelle Jouan Marc Badell Edgar Carcelain Guislaine Martinez Valérie Autran Brigitte Gicquel Brigitte |
| author_facet | Ottenhoff Tom Arend Sandra M Bricaire François Truffot Chantal Sellier Pierre Mauger Isabelle Jouan Marc Badell Edgar Carcelain Guislaine Martinez Valérie Autran Brigitte Gicquel Brigitte |
| author_sort | Ottenhoff Tom |
| collection | DOAJ |
| description | <p>Abstract</p> <p>Background</p> <p>The identification of antigens able to differentiate tuberculosis (TB) disease from TB infection would be valuable. Cellular and humoral immune responses to Erp (Exported repetitive protein) – a recently identified <it>M. tuberculosis </it>protein – have not yet been investigated in humans and may contribute to this aim.</p> <p>Methods</p> <p>We analyzed the cellular and humoral immune responses to Erp, ESAT-6, Ag85B and PPD in TB patients, in BCG<sup>+ </sup>individuals without infection, BCG<sup>+ </sup>individuals with latent TB infection (LTBI) and BCG<sup>- </sup>controls. We used lymphoproliferation, ELISpot IFN-γ, cytokine production assays and detection of specific human antibodies against recombinant <it>M. tuberculosis </it>proteins.</p> <p>Results</p> <p>We included 22 TB patients, 9 BCG<sup>+ </sup>individuals without TB infection, 7 LTBI and 7 BCG<sup>- </sup>controls. Erp-specific T cell counts were higher in LTBI than in the other groups. Erp-specific T cell counts were higher in LTBI subjects than TB patients (median positive frequency of 211 SFC/10<sup>6 </sup>PBMC (range 118–2000) for LTBI subjects compared to 80 SFC/10<sup>6 </sup>PBMC (range 50–191), p = 0.019); responses to PPD and ESAT-6 antigens did not differ between these groups. IFN-γ secretion after Erp stimulation differed between TB patients and LTBI subjects (p = 0.02). Moreover, LTBI subjects but not TB patients or healthy subjects produced IgG3 against Erp.</p> <p>Conclusion</p> <p>The frequencies of IFN-γ-producing specific T cells, the IFN-γ secretion and the production of IgG3 after Erp stimulation are higher in LTBI subjects than in TB patients, whereas PPD and ESAT-6 are not.</p> |
| first_indexed | 2024-12-11T12:05:20Z |
| format | Article |
| id | doaj.art-82192e25b81f4fc79fb13997ec62026f |
| institution | Directory Open Access Journal |
| issn | 1471-2334 |
| language | English |
| last_indexed | 2024-12-11T12:05:20Z |
| publishDate | 2007-07-01 |
| publisher | BMC |
| record_format | Article |
| series | BMC Infectious Diseases |
| spelling | doaj.art-82192e25b81f4fc79fb13997ec62026f2022-12-22T01:07:57ZengBMCBMC Infectious Diseases1471-23342007-07-01718310.1186/1471-2334-7-83T-cell and serological responses to Erp, an exported <it>Mycobacterium tuberculosis </it>protein, in tuberculosis patients and healthy individualsOttenhoff TomArend Sandra MBricaire FrançoisTruffot ChantalSellier PierreMauger IsabelleJouan MarcBadell EdgarCarcelain GuislaineMartinez ValérieAutran BrigitteGicquel Brigitte<p>Abstract</p> <p>Background</p> <p>The identification of antigens able to differentiate tuberculosis (TB) disease from TB infection would be valuable. Cellular and humoral immune responses to Erp (Exported repetitive protein) – a recently identified <it>M. tuberculosis </it>protein – have not yet been investigated in humans and may contribute to this aim.</p> <p>Methods</p> <p>We analyzed the cellular and humoral immune responses to Erp, ESAT-6, Ag85B and PPD in TB patients, in BCG<sup>+ </sup>individuals without infection, BCG<sup>+ </sup>individuals with latent TB infection (LTBI) and BCG<sup>- </sup>controls. We used lymphoproliferation, ELISpot IFN-γ, cytokine production assays and detection of specific human antibodies against recombinant <it>M. tuberculosis </it>proteins.</p> <p>Results</p> <p>We included 22 TB patients, 9 BCG<sup>+ </sup>individuals without TB infection, 7 LTBI and 7 BCG<sup>- </sup>controls. Erp-specific T cell counts were higher in LTBI than in the other groups. Erp-specific T cell counts were higher in LTBI subjects than TB patients (median positive frequency of 211 SFC/10<sup>6 </sup>PBMC (range 118–2000) for LTBI subjects compared to 80 SFC/10<sup>6 </sup>PBMC (range 50–191), p = 0.019); responses to PPD and ESAT-6 antigens did not differ between these groups. IFN-γ secretion after Erp stimulation differed between TB patients and LTBI subjects (p = 0.02). Moreover, LTBI subjects but not TB patients or healthy subjects produced IgG3 against Erp.</p> <p>Conclusion</p> <p>The frequencies of IFN-γ-producing specific T cells, the IFN-γ secretion and the production of IgG3 after Erp stimulation are higher in LTBI subjects than in TB patients, whereas PPD and ESAT-6 are not.</p>http://www.biomedcentral.com/1471-2334/7/83 |
| spellingShingle | Ottenhoff Tom Arend Sandra M Bricaire François Truffot Chantal Sellier Pierre Mauger Isabelle Jouan Marc Badell Edgar Carcelain Guislaine Martinez Valérie Autran Brigitte Gicquel Brigitte T-cell and serological responses to Erp, an exported <it>Mycobacterium tuberculosis </it>protein, in tuberculosis patients and healthy individuals BMC Infectious Diseases |
| title | T-cell and serological responses to Erp, an exported <it>Mycobacterium tuberculosis </it>protein, in tuberculosis patients and healthy individuals |
| title_full | T-cell and serological responses to Erp, an exported <it>Mycobacterium tuberculosis </it>protein, in tuberculosis patients and healthy individuals |
| title_fullStr | T-cell and serological responses to Erp, an exported <it>Mycobacterium tuberculosis </it>protein, in tuberculosis patients and healthy individuals |
| title_full_unstemmed | T-cell and serological responses to Erp, an exported <it>Mycobacterium tuberculosis </it>protein, in tuberculosis patients and healthy individuals |
| title_short | T-cell and serological responses to Erp, an exported <it>Mycobacterium tuberculosis </it>protein, in tuberculosis patients and healthy individuals |
| title_sort | t cell and serological responses to erp an exported it mycobacterium tuberculosis it protein in tuberculosis patients and healthy individuals |
| url | http://www.biomedcentral.com/1471-2334/7/83 |
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