Loading IR820 Using Multifunctional Dendrimers with Enhanced Stability and Specificity
Cyanine dyes are promising candidates in biomedical applications. Although various delivery systems have been developed to enhance their properties, their dendrimer-based delivery systems are seldom investigated. Herein, amine-terminated generation 5 poly(amidoamine) (G5.NH2) dendrimers and new indo...
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| Format: | Article |
| Language: | English |
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MDPI AG
2018-06-01
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| Series: | Pharmaceutics |
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| Online Access: | http://www.mdpi.com/1999-4923/10/3/77 |
| _version_ | 1811184533700083712 |
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| author | Hui Liu Jingjing Wang |
| author_facet | Hui Liu Jingjing Wang |
| author_sort | Hui Liu |
| collection | DOAJ |
| description | Cyanine dyes are promising candidates in biomedical applications. Although various delivery systems have been developed to enhance their properties, their dendrimer-based delivery systems are seldom investigated. Herein, amine-terminated generation 5 poly(amidoamine) (G5.NH2) dendrimers and new indocyanine green (IR820) dyes were chosen as models to study the loading ability of dendrimers for cyanine dynes. G5.NH2 dendrimers were pre-modified with arginine-glycine-aspartic (RGD) peptides, poly(ethylene glycol) chains, and acetyl groups to be endowed with cancer cell specificity and biocompatibility. The formed Ac-PR dendrimers were used to load IR820, followed by thorough characterization. The loaded number of IR820 was estimated to be 6.7 per dendrimer. The stability of IR820 was improved through dendrimer loading, which was proved by their UV-vis spectra under different kinds of storage conditions. In addition, the formed Ac-PR dendrimers can retain the loaded IR820 effectively. Their cytocompatibility was desirable under the studied conditions. Their cellular uptake behaviors were demonstrated to be enhanced by RGD modification, showing concentration-, co-incubation time-, and αvβ3 integrin receptor-dependent properties, displaying a cytoplasm-location. The findings from this work demonstrated the versatile loading and delivery capacity of dendrimers for near-infrared (NIR) dyes, providing fundamental data for the development of dendrimer/NIR dye systems for biomedical applications, especially for cancer theranostic applications. |
| first_indexed | 2024-04-11T13:13:41Z |
| format | Article |
| id | doaj.art-821d771505fe45b58ff8357d92a4e88c |
| institution | Directory Open Access Journal |
| issn | 1999-4923 |
| language | English |
| last_indexed | 2024-04-11T13:13:41Z |
| publishDate | 2018-06-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Pharmaceutics |
| spelling | doaj.art-821d771505fe45b58ff8357d92a4e88c2022-12-22T04:22:27ZengMDPI AGPharmaceutics1999-49232018-06-011037710.3390/pharmaceutics10030077pharmaceutics10030077Loading IR820 Using Multifunctional Dendrimers with Enhanced Stability and SpecificityHui Liu0Jingjing Wang1Institute for Clean Energy and Advanced Materials, Faculty of Materials and Energy, Southwest University, Chongqing 400715, ChinaInstitute for Clean Energy and Advanced Materials, Faculty of Materials and Energy, Southwest University, Chongqing 400715, ChinaCyanine dyes are promising candidates in biomedical applications. Although various delivery systems have been developed to enhance their properties, their dendrimer-based delivery systems are seldom investigated. Herein, amine-terminated generation 5 poly(amidoamine) (G5.NH2) dendrimers and new indocyanine green (IR820) dyes were chosen as models to study the loading ability of dendrimers for cyanine dynes. G5.NH2 dendrimers were pre-modified with arginine-glycine-aspartic (RGD) peptides, poly(ethylene glycol) chains, and acetyl groups to be endowed with cancer cell specificity and biocompatibility. The formed Ac-PR dendrimers were used to load IR820, followed by thorough characterization. The loaded number of IR820 was estimated to be 6.7 per dendrimer. The stability of IR820 was improved through dendrimer loading, which was proved by their UV-vis spectra under different kinds of storage conditions. In addition, the formed Ac-PR dendrimers can retain the loaded IR820 effectively. Their cytocompatibility was desirable under the studied conditions. Their cellular uptake behaviors were demonstrated to be enhanced by RGD modification, showing concentration-, co-incubation time-, and αvβ3 integrin receptor-dependent properties, displaying a cytoplasm-location. The findings from this work demonstrated the versatile loading and delivery capacity of dendrimers for near-infrared (NIR) dyes, providing fundamental data for the development of dendrimer/NIR dye systems for biomedical applications, especially for cancer theranostic applications.http://www.mdpi.com/1999-4923/10/3/77poly(amidoamine) dendrimersnew indocyanine green (IR820)stabilitytargeted deliverycancer cells |
| spellingShingle | Hui Liu Jingjing Wang Loading IR820 Using Multifunctional Dendrimers with Enhanced Stability and Specificity Pharmaceutics poly(amidoamine) dendrimers new indocyanine green (IR820) stability targeted delivery cancer cells |
| title | Loading IR820 Using Multifunctional Dendrimers with Enhanced Stability and Specificity |
| title_full | Loading IR820 Using Multifunctional Dendrimers with Enhanced Stability and Specificity |
| title_fullStr | Loading IR820 Using Multifunctional Dendrimers with Enhanced Stability and Specificity |
| title_full_unstemmed | Loading IR820 Using Multifunctional Dendrimers with Enhanced Stability and Specificity |
| title_short | Loading IR820 Using Multifunctional Dendrimers with Enhanced Stability and Specificity |
| title_sort | loading ir820 using multifunctional dendrimers with enhanced stability and specificity |
| topic | poly(amidoamine) dendrimers new indocyanine green (IR820) stability targeted delivery cancer cells |
| url | http://www.mdpi.com/1999-4923/10/3/77 |
| work_keys_str_mv | AT huiliu loadingir820usingmultifunctionaldendrimerswithenhancedstabilityandspecificity AT jingjingwang loadingir820usingmultifunctionaldendrimerswithenhancedstabilityandspecificity |