Effects of hypoxia on the mRNA expressions of TRAIL-mediated cell death related genes in hypoxia-tolerant rodent (Nannospalax nehringi) and some characteristics of these proteins
The aim of the present study was to determine the mRNA expression profiles of TRAIL-mediated cell death related genes of the hypoxia-tolerant rodent Nannospalax nehringi under hypoxia. The nucleotide and amino acid sequences of these genes were identified. Captured Nannospalax nehringi were randomly...
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| Format: | Article |
| Language: | English |
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University of Belgrade, University of Novi Sad
2021-01-01
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| Series: | Archives of Biological Sciences |
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| Online Access: | http://www.doiserbia.nb.rs/img/doi/0354-4664/2021/0354-46642000053Y.pdf |
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| author | Yıldız Barış Kamiloğlu Nadide Nabil Öziç Cem Coşkun Yüksel |
| author_facet | Yıldız Barış Kamiloğlu Nadide Nabil Öziç Cem Coşkun Yüksel |
| author_sort | Yıldız Barış |
| collection | DOAJ |
| description | The aim of the present study was to determine the mRNA expression profiles of TRAIL-mediated cell death related genes of the hypoxia-tolerant rodent Nannospalax nehringi under hypoxia. The nucleotide and amino acid sequences of these genes were identified. Captured Nannospalax nehringi were randomly divided into normoxia and hypoxia groups. The hypoxia group was exposed to a 7% O2 + 93% N2 gas mixture for 52 h, while animals in the normoxia group were housed under normoxic conditions. Total RNAs were isolated from brain and lung tissues. The cIAP-1, cIAP-2, XIAP, DcR1, DcR2, FLIP and OPG genes were PCR amplified, and the cIAP-1, cIAP-2, XIAP, OPG, TRAIL and FLIP genes were sequenced. Sequenced genes were translated into amino acid sequences and compared with reliable sequences of closely similar species. The genes in the brain were regulated for protection against hypoxia; however, the genes in the lung were regulated differently. Many mutations and insertions were observed on the conserved regions of the cIAP-1, cIAP-2, XIAP, OPG and TRAIL genes in N. nehringi. We propose that these gene mutations and insertions contribute to the anti-hypoxic properties of N. nehringi. |
| first_indexed | 2024-12-18T00:15:15Z |
| format | Article |
| id | doaj.art-821df39c62ff455ea1de3d941f1190e9 |
| institution | Directory Open Access Journal |
| issn | 0354-4664 1821-4339 |
| language | English |
| last_indexed | 2024-12-18T00:15:15Z |
| publishDate | 2021-01-01 |
| publisher | University of Belgrade, University of Novi Sad |
| record_format | Article |
| series | Archives of Biological Sciences |
| spelling | doaj.art-821df39c62ff455ea1de3d941f1190e92022-12-21T21:27:32ZengUniversity of Belgrade, University of Novi SadArchives of Biological Sciences0354-46641821-43392021-01-0173151510.2298/ABS201119053Y0354-46642000053YEffects of hypoxia on the mRNA expressions of TRAIL-mediated cell death related genes in hypoxia-tolerant rodent (Nannospalax nehringi) and some characteristics of these proteinsYıldız Barış0Kamiloğlu Nadide Nabil1Öziç Cem2Coşkun Yüksel3Department of Physiology, Institute of Health Sciences, Kafkas University, Kars, TurkeyDepartment of Physiology, Faculty of Veterinary Medicine, Kafkas University, Kars, TurkeyDepartment of Medical Biology, Faculty of Medicine, Kafkas University, Kars, TurkeyDepartment of Biology, Faculty of Science, Dicle University, Diyarbakır, TurkeyThe aim of the present study was to determine the mRNA expression profiles of TRAIL-mediated cell death related genes of the hypoxia-tolerant rodent Nannospalax nehringi under hypoxia. The nucleotide and amino acid sequences of these genes were identified. Captured Nannospalax nehringi were randomly divided into normoxia and hypoxia groups. The hypoxia group was exposed to a 7% O2 + 93% N2 gas mixture for 52 h, while animals in the normoxia group were housed under normoxic conditions. Total RNAs were isolated from brain and lung tissues. The cIAP-1, cIAP-2, XIAP, DcR1, DcR2, FLIP and OPG genes were PCR amplified, and the cIAP-1, cIAP-2, XIAP, OPG, TRAIL and FLIP genes were sequenced. Sequenced genes were translated into amino acid sequences and compared with reliable sequences of closely similar species. The genes in the brain were regulated for protection against hypoxia; however, the genes in the lung were regulated differently. Many mutations and insertions were observed on the conserved regions of the cIAP-1, cIAP-2, XIAP, OPG and TRAIL genes in N. nehringi. We propose that these gene mutations and insertions contribute to the anti-hypoxic properties of N. nehringi.http://www.doiserbia.nb.rs/img/doi/0354-4664/2021/0354-46642000053Y.pdfapoptosisnannospalax nehringihypoxiatnf-related apoptosis-inducing ligand(trail)inhibitors of apoptosis (iaps)flice-like inhibitory protein (flip) |
| spellingShingle | Yıldız Barış Kamiloğlu Nadide Nabil Öziç Cem Coşkun Yüksel Effects of hypoxia on the mRNA expressions of TRAIL-mediated cell death related genes in hypoxia-tolerant rodent (Nannospalax nehringi) and some characteristics of these proteins Archives of Biological Sciences apoptosis nannospalax nehringi hypoxia tnf-related apoptosis-inducing ligand(trail) inhibitors of apoptosis (iaps) flice-like inhibitory protein (flip) |
| title | Effects of hypoxia on the mRNA expressions of TRAIL-mediated cell death related genes in hypoxia-tolerant rodent (Nannospalax nehringi) and some characteristics of these proteins |
| title_full | Effects of hypoxia on the mRNA expressions of TRAIL-mediated cell death related genes in hypoxia-tolerant rodent (Nannospalax nehringi) and some characteristics of these proteins |
| title_fullStr | Effects of hypoxia on the mRNA expressions of TRAIL-mediated cell death related genes in hypoxia-tolerant rodent (Nannospalax nehringi) and some characteristics of these proteins |
| title_full_unstemmed | Effects of hypoxia on the mRNA expressions of TRAIL-mediated cell death related genes in hypoxia-tolerant rodent (Nannospalax nehringi) and some characteristics of these proteins |
| title_short | Effects of hypoxia on the mRNA expressions of TRAIL-mediated cell death related genes in hypoxia-tolerant rodent (Nannospalax nehringi) and some characteristics of these proteins |
| title_sort | effects of hypoxia on the mrna expressions of trail mediated cell death related genes in hypoxia tolerant rodent nannospalax nehringi and some characteristics of these proteins |
| topic | apoptosis nannospalax nehringi hypoxia tnf-related apoptosis-inducing ligand(trail) inhibitors of apoptosis (iaps) flice-like inhibitory protein (flip) |
| url | http://www.doiserbia.nb.rs/img/doi/0354-4664/2021/0354-46642000053Y.pdf |
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