Relationship between Microflora Changes and Mammary Lipid Metabolism in Dairy Cows with Mastitis
Dairy mastitis is an inflammatory reaction caused by mechanical injury and stress within the mammary gland, during which microbial changes and abnormal lipid metabolism occur. However, the underlying mechanism is still unclear. The present study used a combination of 16S rDNA sequencing technology a...
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MDPI AG
2023-08-01
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| Series: | Animals |
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| Online Access: | https://www.mdpi.com/2076-2615/13/17/2773 |
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| author | Yang Luo Zhiwei Kong Bin Yang Fang He Cheng Huan Jianbo Li Kangle Yi |
| author_facet | Yang Luo Zhiwei Kong Bin Yang Fang He Cheng Huan Jianbo Li Kangle Yi |
| author_sort | Yang Luo |
| collection | DOAJ |
| description | Dairy mastitis is an inflammatory reaction caused by mechanical injury and stress within the mammary gland, during which microbial changes and abnormal lipid metabolism occur. However, the underlying mechanism is still unclear. The present study used a combination of 16S rDNA sequencing technology and lipidomics techniques to reveal the effects of mastitis on lactic microbiota and metabolites in the milk of dairy cows. Twenty multiparous Holstein dairy cows (2–3 parities) with an average body weight of 580 ± 30 kg were selected for this study. The dairy cows were allocated to control group (<5 × 10<sup>4</sup> cells /mL)) and mastitis group (>5 × 10<sup>6</sup> cells /mL) based on the somatic cell count. The results showed that mastitis caused a decrease trend in milk production (<i>p</i> = 0.058). The results of the 16 s sequencing indicated a significant decrease (<i>p <</i> 0.05) in the number of <i>Proteobacteria</i>, <i>Tenericutes</i> colonized in mastitis milk, and the number of <i>Firmicutes</i>, <i>Bacteroidetes</i> and <i>Actinobacteria</i> communities increased significantly (<i>p <</i> 0.05). The lipidomics results revealed that the changes in lipid content in mastitis milk were correlated with arachidonic acid metabolism, α -linolenic acid metabolism and glycerol phospholipid metabolism. The results showed that mastitis may cause abnormal lipid metabolism in milk by regulating the diversity of milk microflora, and ultimately affect the milk quality. |
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| language | English |
| last_indexed | 2024-03-10T23:28:45Z |
| publishDate | 2023-08-01 |
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| series | Animals |
| spelling | doaj.art-821f835e1fd343aebf891ce52a3f98ab2023-11-19T07:47:12ZengMDPI AGAnimals2076-26152023-08-011317277310.3390/ani13172773Relationship between Microflora Changes and Mammary Lipid Metabolism in Dairy Cows with MastitisYang Luo0Zhiwei Kong1Bin Yang2Fang He3Cheng Huan4Jianbo Li5Kangle Yi6College of Animal Science and Technology, Guangxi University, Nanning 530004, ChinaCollege of Animal Science and Technology, Guangxi University, Nanning 530004, ChinaSchool of Biological and Chemical Engineering, Zhejiang University of Science and Technology, Hangzhou 310023, ChinaHunan Institute of Animal and Veterinary Science, Changsha 410131, ChinaHunan Institute of Animal and Veterinary Science, Changsha 410131, ChinaHunan Institute of Animal and Veterinary Science, Changsha 410131, ChinaHunan Institute of Animal and Veterinary Science, Changsha 410131, ChinaDairy mastitis is an inflammatory reaction caused by mechanical injury and stress within the mammary gland, during which microbial changes and abnormal lipid metabolism occur. However, the underlying mechanism is still unclear. The present study used a combination of 16S rDNA sequencing technology and lipidomics techniques to reveal the effects of mastitis on lactic microbiota and metabolites in the milk of dairy cows. Twenty multiparous Holstein dairy cows (2–3 parities) with an average body weight of 580 ± 30 kg were selected for this study. The dairy cows were allocated to control group (<5 × 10<sup>4</sup> cells /mL)) and mastitis group (>5 × 10<sup>6</sup> cells /mL) based on the somatic cell count. The results showed that mastitis caused a decrease trend in milk production (<i>p</i> = 0.058). The results of the 16 s sequencing indicated a significant decrease (<i>p <</i> 0.05) in the number of <i>Proteobacteria</i>, <i>Tenericutes</i> colonized in mastitis milk, and the number of <i>Firmicutes</i>, <i>Bacteroidetes</i> and <i>Actinobacteria</i> communities increased significantly (<i>p <</i> 0.05). The lipidomics results revealed that the changes in lipid content in mastitis milk were correlated with arachidonic acid metabolism, α -linolenic acid metabolism and glycerol phospholipid metabolism. The results showed that mastitis may cause abnormal lipid metabolism in milk by regulating the diversity of milk microflora, and ultimately affect the milk quality.https://www.mdpi.com/2076-2615/13/17/2773mastitisdairy cowmicrobial diversitylipid metabolism |
| spellingShingle | Yang Luo Zhiwei Kong Bin Yang Fang He Cheng Huan Jianbo Li Kangle Yi Relationship between Microflora Changes and Mammary Lipid Metabolism in Dairy Cows with Mastitis Animals mastitis dairy cow microbial diversity lipid metabolism |
| title | Relationship between Microflora Changes and Mammary Lipid Metabolism in Dairy Cows with Mastitis |
| title_full | Relationship between Microflora Changes and Mammary Lipid Metabolism in Dairy Cows with Mastitis |
| title_fullStr | Relationship between Microflora Changes and Mammary Lipid Metabolism in Dairy Cows with Mastitis |
| title_full_unstemmed | Relationship between Microflora Changes and Mammary Lipid Metabolism in Dairy Cows with Mastitis |
| title_short | Relationship between Microflora Changes and Mammary Lipid Metabolism in Dairy Cows with Mastitis |
| title_sort | relationship between microflora changes and mammary lipid metabolism in dairy cows with mastitis |
| topic | mastitis dairy cow microbial diversity lipid metabolism |
| url | https://www.mdpi.com/2076-2615/13/17/2773 |
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