Rapid Detection of Escherichia coli in Water Samples by Polymerase Chain Reaction using 16E1 and 16E2 Primers
The detection of Escherichia coli in various water samples by Polymerase Chain Reaction method using 16E1 and 16E2 primers has been carried out. It was found that four out of ten samples were detected Escherichia coli and revealed the presence of the amplified product of the size 584 base pairs. Con...
| Main Authors: | , , |
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| Format: | Article |
| Language: | English |
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Universitas Indonesia
2010-04-01
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| Series: | Makara Seri Sains |
| Subjects: | |
| Online Access: | http://journal.ui.ac.id/science/article/view/474 |
| _version_ | 1811245360723525632 |
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| author | Atiek Soemiati Anglia Puspaningrum Maksum R |
| author_facet | Atiek Soemiati Anglia Puspaningrum Maksum R |
| author_sort | Atiek Soemiati |
| collection | DOAJ |
| description | The detection of Escherichia coli in various water samples by Polymerase Chain Reaction method using 16E1 and 16E2 primers has been carried out. It was found that four out of ten samples were detected Escherichia coli and revealed the presence of the amplified product of the size 584 base pairs. Confirmation test of PCR result was also done using the conventional culturing method. The results of this study showed that the PCR assay was specific and faster than the conventional culturing method for detection of Escherichia coli. |
| first_indexed | 2024-04-12T14:39:00Z |
| format | Article |
| id | doaj.art-82202e3fbdfc49d3a64abfe0682a0770 |
| institution | Directory Open Access Journal |
| issn | 1693-6671 |
| language | English |
| last_indexed | 2024-04-12T14:39:00Z |
| publishDate | 2010-04-01 |
| publisher | Universitas Indonesia |
| record_format | Article |
| series | Makara Seri Sains |
| spelling | doaj.art-82202e3fbdfc49d3a64abfe0682a07702022-12-22T03:28:57ZengUniversitas IndonesiaMakara Seri Sains1693-66712010-04-011413943Rapid Detection of Escherichia coli in Water Samples by Polymerase Chain Reaction using 16E1 and 16E2 PrimersAtiek SoemiatiAnglia PuspaningrumMaksum RThe detection of Escherichia coli in various water samples by Polymerase Chain Reaction method using 16E1 and 16E2 primers has been carried out. It was found that four out of ten samples were detected Escherichia coli and revealed the presence of the amplified product of the size 584 base pairs. Confirmation test of PCR result was also done using the conventional culturing method. The results of this study showed that the PCR assay was specific and faster than the conventional culturing method for detection of Escherichia coli.journal.ui.ac.id/science/article/view/47416E1 and 16E2 primersEscherichia coliPCRwater |
| spellingShingle | Atiek Soemiati Anglia Puspaningrum Maksum R Rapid Detection of Escherichia coli in Water Samples by Polymerase Chain Reaction using 16E1 and 16E2 Primers Makara Seri Sains 16E1 and 16E2 primers Escherichia coli PCR water |
| title | Rapid Detection of Escherichia coli in Water Samples by Polymerase Chain Reaction using 16E1 and 16E2 Primers |
| title_full | Rapid Detection of Escherichia coli in Water Samples by Polymerase Chain Reaction using 16E1 and 16E2 Primers |
| title_fullStr | Rapid Detection of Escherichia coli in Water Samples by Polymerase Chain Reaction using 16E1 and 16E2 Primers |
| title_full_unstemmed | Rapid Detection of Escherichia coli in Water Samples by Polymerase Chain Reaction using 16E1 and 16E2 Primers |
| title_short | Rapid Detection of Escherichia coli in Water Samples by Polymerase Chain Reaction using 16E1 and 16E2 Primers |
| title_sort | rapid detection of escherichia coli in water samples by polymerase chain reaction using 16e1 and 16e2 primers |
| topic | 16E1 and 16E2 primers Escherichia coli PCR water |
| url | http://journal.ui.ac.id/science/article/view/474 |
| work_keys_str_mv | AT atieksoemiati rapiddetectionofescherichiacoliinwatersamplesbypolymerasechainreactionusing16e1and16e2primers AT angliapuspaningrum rapiddetectionofescherichiacoliinwatersamplesbypolymerasechainreactionusing16e1and16e2primers AT maksumr rapiddetectionofescherichiacoliinwatersamplesbypolymerasechainreactionusing16e1and16e2primers |