Chaperone-tip adhesin complex is vital for synergistic activation of CFA/I fimbriae biogenesis.
Colonization factor CFA/I defines the major adhesive fimbriae of enterotoxigenic Escherichia coli and mediates bacterial attachment to host intestinal epithelial cells. The CFA/I fimbria consists of a tip-localized minor adhesive subunit, CfaE, and thousands of copies of the major subunit CfaB polym...
| Main Authors: | , , , , , , , , , , , , |
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| Format: | Article |
| Language: | English |
| Published: |
Public Library of Science (PLoS)
2020-10-01
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| Series: | PLoS Pathogens |
| Online Access: | https://doi.org/10.1371/journal.ppat.1008848 |
| _version_ | 1818734941474652160 |
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| author | Li-Hui He Hao Wang Yang Liu Mei Kang Tao Li Chang-Cheng Li Ai-Ping Tong Yi-Bo Zhu Ying-Jie Song Stephen J Savarino Michael G Prouty Di Xia Rui Bao |
| author_facet | Li-Hui He Hao Wang Yang Liu Mei Kang Tao Li Chang-Cheng Li Ai-Ping Tong Yi-Bo Zhu Ying-Jie Song Stephen J Savarino Michael G Prouty Di Xia Rui Bao |
| author_sort | Li-Hui He |
| collection | DOAJ |
| description | Colonization factor CFA/I defines the major adhesive fimbriae of enterotoxigenic Escherichia coli and mediates bacterial attachment to host intestinal epithelial cells. The CFA/I fimbria consists of a tip-localized minor adhesive subunit, CfaE, and thousands of copies of the major subunit CfaB polymerized into an ordered helical rod. Biosynthesis of CFA/I fimbriae requires the assistance of the periplasmic chaperone CfaA and outer membrane usher CfaC. Although the CfaE subunit is proposed to initiate the assembly of CFA/I fimbriae, how it performs this function remains elusive. Here, we report the establishment of an in vitro assay for CFA/I fimbria assembly and show that stabilized CfaA-CfaB and CfaA-CfaE binary complexes together with CfaC are sufficient to drive fimbria formation. The presence of both CfaA-CfaE and CfaC accelerates fimbria formation, while the absence of either component leads to linearized CfaB polymers in vitro. We further report the crystal structure of the stabilized CfaA-CfaE complex, revealing features unique for biogenesis of Class 5 fimbriae. |
| first_indexed | 2024-12-18T00:13:22Z |
| format | Article |
| id | doaj.art-82214539a7c94c32a52c6d9210147c44 |
| institution | Directory Open Access Journal |
| issn | 1553-7366 1553-7374 |
| language | English |
| last_indexed | 2024-12-18T00:13:22Z |
| publishDate | 2020-10-01 |
| publisher | Public Library of Science (PLoS) |
| record_format | Article |
| series | PLoS Pathogens |
| spelling | doaj.art-82214539a7c94c32a52c6d9210147c442022-12-21T21:27:36ZengPublic Library of Science (PLoS)PLoS Pathogens1553-73661553-73742020-10-011610e100884810.1371/journal.ppat.1008848Chaperone-tip adhesin complex is vital for synergistic activation of CFA/I fimbriae biogenesis.Li-Hui HeHao WangYang LiuMei KangTao LiChang-Cheng LiAi-Ping TongYi-Bo ZhuYing-Jie SongStephen J SavarinoMichael G ProutyDi XiaRui BaoColonization factor CFA/I defines the major adhesive fimbriae of enterotoxigenic Escherichia coli and mediates bacterial attachment to host intestinal epithelial cells. The CFA/I fimbria consists of a tip-localized minor adhesive subunit, CfaE, and thousands of copies of the major subunit CfaB polymerized into an ordered helical rod. Biosynthesis of CFA/I fimbriae requires the assistance of the periplasmic chaperone CfaA and outer membrane usher CfaC. Although the CfaE subunit is proposed to initiate the assembly of CFA/I fimbriae, how it performs this function remains elusive. Here, we report the establishment of an in vitro assay for CFA/I fimbria assembly and show that stabilized CfaA-CfaB and CfaA-CfaE binary complexes together with CfaC are sufficient to drive fimbria formation. The presence of both CfaA-CfaE and CfaC accelerates fimbria formation, while the absence of either component leads to linearized CfaB polymers in vitro. We further report the crystal structure of the stabilized CfaA-CfaE complex, revealing features unique for biogenesis of Class 5 fimbriae.https://doi.org/10.1371/journal.ppat.1008848 |
| spellingShingle | Li-Hui He Hao Wang Yang Liu Mei Kang Tao Li Chang-Cheng Li Ai-Ping Tong Yi-Bo Zhu Ying-Jie Song Stephen J Savarino Michael G Prouty Di Xia Rui Bao Chaperone-tip adhesin complex is vital for synergistic activation of CFA/I fimbriae biogenesis. PLoS Pathogens |
| title | Chaperone-tip adhesin complex is vital for synergistic activation of CFA/I fimbriae biogenesis. |
| title_full | Chaperone-tip adhesin complex is vital for synergistic activation of CFA/I fimbriae biogenesis. |
| title_fullStr | Chaperone-tip adhesin complex is vital for synergistic activation of CFA/I fimbriae biogenesis. |
| title_full_unstemmed | Chaperone-tip adhesin complex is vital for synergistic activation of CFA/I fimbriae biogenesis. |
| title_short | Chaperone-tip adhesin complex is vital for synergistic activation of CFA/I fimbriae biogenesis. |
| title_sort | chaperone tip adhesin complex is vital for synergistic activation of cfa i fimbriae biogenesis |
| url | https://doi.org/10.1371/journal.ppat.1008848 |
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