The Simultaneous Effect of Linum Usitatissimum Supplementation and Aerobic Training on 6-Methylguanine and ATP in the Endothelial Aorta and Heart Tissues in Rats Poisoned with H2O2
Background and Objective: As a high-energy demanding tissue, the heart is exposed to a high level of ROS molecules such as H2O2, leading to cardiovascular disorders through damaging macromolecules such as DNA and disrupting ATP production. Hence, this study aimed to investigate the simultaneous effe...
| Main Authors: | , , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
Golestan University of Medical Sciences
2021-09-01
|
| Series: | Jorjani Biomedicine Journal |
| Subjects: | |
| Online Access: | http://goums.ac.ir/jorjanijournal/article-1-823-en.html |
| _version_ | 1819005912364351488 |
|---|---|
| author | Seyed Mostafa rezaei Bahram Abedi Hoseyn Fatolahi |
| author_facet | Seyed Mostafa rezaei Bahram Abedi Hoseyn Fatolahi |
| author_sort | Seyed Mostafa rezaei |
| collection | DOAJ |
| description | Background and Objective: As a high-energy demanding tissue, the heart is exposed to a high level of ROS molecules such as H2O2, leading to cardiovascular disorders through damaging macromolecules such as DNA and disrupting ATP production. Hence, this study aimed to investigate the simultaneous effect of aerobic exercise (Ae) and Linum Usitatissimum (Lu) supplementation on DNA damage and ATP synthesis in heart and aorta endothelial tissues in rats poisoned with hydrogen peroxide (H2O2).
Material and Methods: 56 male Wistar Albino rats were randomly divided into 7 groups, including HC (Healthy Control), TC (Toxicated Control), Lu1 (Received 5 mg/kg of Lu), Lu2 (Received 10 mg/kg of Lu), Ae (Received only Aerobic Exercise), Ae Lu1, and Ae Lu2. Then, all groups got poisoned by H2O2 except HC. Next, they received Linum Usitatissimum (Lu) supplementation and Low-Intensity Interval Training (LIIT). Finally, 24h after the last treatment session, the level of 6-methylguanine (6MG) and ATP were measured via the ELISA technique in cardiovascular tissue.
Results: The findings determined that Lu supplementation and Ae significantly diminish the 6-methyl guanine level in endothelial (F=111.3, p=0.0008, ƞ=0.9823) and heart cells (F=147.9, p=0.0005, ƞ=0.9867). Also, the ATP level was increased significantly in endothelial (F=342.6, p=0.0003, ƞ=0.9942) and heart cells (F=135.1, p=0.0013, ƞ=0.9854). However, no considerable changes were found for both factors in groups who received Ae or Lu singularly.
Conclusion: The study showed that concurrent administration of Lu and Ae could exert dynamic cardioprotective properties through their antioxidant effects. |
| first_indexed | 2024-12-21T00:00:19Z |
| format | Article |
| id | doaj.art-8227dec799e94110abef96c704e964f9 |
| institution | Directory Open Access Journal |
| issn | 2645-3509 |
| language | English |
| last_indexed | 2024-12-21T00:00:19Z |
| publishDate | 2021-09-01 |
| publisher | Golestan University of Medical Sciences |
| record_format | Article |
| series | Jorjani Biomedicine Journal |
| spelling | doaj.art-8227dec799e94110abef96c704e964f92022-12-21T19:22:37ZengGolestan University of Medical SciencesJorjani Biomedicine Journal2645-35092021-09-01931222The Simultaneous Effect of Linum Usitatissimum Supplementation and Aerobic Training on 6-Methylguanine and ATP in the Endothelial Aorta and Heart Tissues in Rats Poisoned with H2O2Seyed Mostafa rezaei0Bahram Abedi1Hoseyn Fatolahi2 Ph.D. student, Department of Physical Education and Sport Sciences, Mahallat Branch, Islamic Azad University. Mahallat, Iran Professor, Department of Physical Education and Sport Sciences, Mahallat Branch, Islamic Azad University. Mahallat, Iran Assistant Professor, Department of Physical Education, Pardis Branch, Islamic Azad University, Pardis, Iran Background and Objective: As a high-energy demanding tissue, the heart is exposed to a high level of ROS molecules such as H2O2, leading to cardiovascular disorders through damaging macromolecules such as DNA and disrupting ATP production. Hence, this study aimed to investigate the simultaneous effect of aerobic exercise (Ae) and Linum Usitatissimum (Lu) supplementation on DNA damage and ATP synthesis in heart and aorta endothelial tissues in rats poisoned with hydrogen peroxide (H2O2). Material and Methods: 56 male Wistar Albino rats were randomly divided into 7 groups, including HC (Healthy Control), TC (Toxicated Control), Lu1 (Received 5 mg/kg of Lu), Lu2 (Received 10 mg/kg of Lu), Ae (Received only Aerobic Exercise), Ae Lu1, and Ae Lu2. Then, all groups got poisoned by H2O2 except HC. Next, they received Linum Usitatissimum (Lu) supplementation and Low-Intensity Interval Training (LIIT). Finally, 24h after the last treatment session, the level of 6-methylguanine (6MG) and ATP were measured via the ELISA technique in cardiovascular tissue. Results: The findings determined that Lu supplementation and Ae significantly diminish the 6-methyl guanine level in endothelial (F=111.3, p=0.0008, ƞ=0.9823) and heart cells (F=147.9, p=0.0005, ƞ=0.9867). Also, the ATP level was increased significantly in endothelial (F=342.6, p=0.0003, ƞ=0.9942) and heart cells (F=135.1, p=0.0013, ƞ=0.9854). However, no considerable changes were found for both factors in groups who received Ae or Lu singularly. Conclusion: The study showed that concurrent administration of Lu and Ae could exert dynamic cardioprotective properties through their antioxidant effects.http://goums.ac.ir/jorjanijournal/article-1-823-en.htmlh2o2 toxicitymitochondrialcardiovascular diseasesherbal medicinerehabilitationexercise |
| spellingShingle | Seyed Mostafa rezaei Bahram Abedi Hoseyn Fatolahi The Simultaneous Effect of Linum Usitatissimum Supplementation and Aerobic Training on 6-Methylguanine and ATP in the Endothelial Aorta and Heart Tissues in Rats Poisoned with H2O2 Jorjani Biomedicine Journal h2o2 toxicity mitochondrial cardiovascular diseases herbal medicine rehabilitation exercise |
| title | The Simultaneous Effect of Linum Usitatissimum Supplementation and Aerobic Training on 6-Methylguanine and ATP in the Endothelial Aorta and Heart Tissues in Rats Poisoned with H2O2 |
| title_full | The Simultaneous Effect of Linum Usitatissimum Supplementation and Aerobic Training on 6-Methylguanine and ATP in the Endothelial Aorta and Heart Tissues in Rats Poisoned with H2O2 |
| title_fullStr | The Simultaneous Effect of Linum Usitatissimum Supplementation and Aerobic Training on 6-Methylguanine and ATP in the Endothelial Aorta and Heart Tissues in Rats Poisoned with H2O2 |
| title_full_unstemmed | The Simultaneous Effect of Linum Usitatissimum Supplementation and Aerobic Training on 6-Methylguanine and ATP in the Endothelial Aorta and Heart Tissues in Rats Poisoned with H2O2 |
| title_short | The Simultaneous Effect of Linum Usitatissimum Supplementation and Aerobic Training on 6-Methylguanine and ATP in the Endothelial Aorta and Heart Tissues in Rats Poisoned with H2O2 |
| title_sort | simultaneous effect of linum usitatissimum supplementation and aerobic training on 6 methylguanine and atp in the endothelial aorta and heart tissues in rats poisoned with h2o2 |
| topic | h2o2 toxicity mitochondrial cardiovascular diseases herbal medicine rehabilitation exercise |
| url | http://goums.ac.ir/jorjanijournal/article-1-823-en.html |
| work_keys_str_mv | AT seyedmostafarezaei thesimultaneouseffectoflinumusitatissimumsupplementationandaerobictrainingon6methylguanineandatpintheendothelialaortaandhearttissuesinratspoisonedwithh2o2 AT bahramabedi thesimultaneouseffectoflinumusitatissimumsupplementationandaerobictrainingon6methylguanineandatpintheendothelialaortaandhearttissuesinratspoisonedwithh2o2 AT hoseynfatolahi thesimultaneouseffectoflinumusitatissimumsupplementationandaerobictrainingon6methylguanineandatpintheendothelialaortaandhearttissuesinratspoisonedwithh2o2 AT seyedmostafarezaei simultaneouseffectoflinumusitatissimumsupplementationandaerobictrainingon6methylguanineandatpintheendothelialaortaandhearttissuesinratspoisonedwithh2o2 AT bahramabedi simultaneouseffectoflinumusitatissimumsupplementationandaerobictrainingon6methylguanineandatpintheendothelialaortaandhearttissuesinratspoisonedwithh2o2 AT hoseynfatolahi simultaneouseffectoflinumusitatissimumsupplementationandaerobictrainingon6methylguanineandatpintheendothelialaortaandhearttissuesinratspoisonedwithh2o2 |