Emergence of community-associated methicillin-resistant Staphylococcus aureus ΨUSA300 among Japanese people with HIV, resulted from stepwise mutations in 2010s
Abstract Although infection with the methicillin-resistant Staphylococcus aureus (MRSA) clone USA300 is extremely rare in Japan, the uniquely evolved clone ΨUSA300 has been reported in Japan. An outbreak of a distinct USA300 clone was recently reported in an HIV/AIDS referral hospital in Tokyo. The...
| Main Authors: | , , , , , , , , , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
Nature Portfolio
2023-05-01
|
| Series: | Scientific Reports |
| Online Access: | https://doi.org/10.1038/s41598-023-35171-y |
| _version_ | 1797818073022988288 |
|---|---|
| author | Koh Shinohara Yuki Uehara Katsuji Teruya Takashi Sasaki Tadashi Baba Hidemasa Nakaminami Pegah Kananizadeh Yuh Morimoto Yoshimi Kikuchi Shinichi Oka |
| author_facet | Koh Shinohara Yuki Uehara Katsuji Teruya Takashi Sasaki Tadashi Baba Hidemasa Nakaminami Pegah Kananizadeh Yuh Morimoto Yoshimi Kikuchi Shinichi Oka |
| author_sort | Koh Shinohara |
| collection | DOAJ |
| description | Abstract Although infection with the methicillin-resistant Staphylococcus aureus (MRSA) clone USA300 is extremely rare in Japan, the uniquely evolved clone ΨUSA300 has been reported in Japan. An outbreak of a distinct USA300 clone was recently reported in an HIV/AIDS referral hospital in Tokyo. The present study investigated the evolutionary origin and genetic diversity of USA300-related clones causing regional outbreaks among people living with HIV (PLWHIV) in Tokyo. MRSA isolates collected from PLWHIV in an HIV/AIDS referral center in Tokyo were subjected to whole-genome sequencing and their genetic features were compared with those of previously described USA300 MRSA genomes. Of the 28 MRSAs isolated in 2016–2019, 23 (82.1%) were identified as USA300, with 22 (95.6%) of the latter identified as ΨUSA300. Although the genomic structure of ΨUSA300 was identical to the structures of reference USA300 strains, one clade (cluster A) was found to have acquired 29 previously identified lineage-specific mutations in a stepwise manner. The estimated divergence dates of ΨUSA300 and Cluster A were 2009 and 2012, respectively. These findings suggested that the ΨUSA300 clone had spread among PLWHIVs in Tokyo in the early 2010s, with stepwise acquisition of lineage-specific nonsynonymous mutations. |
| first_indexed | 2024-03-13T09:02:45Z |
| format | Article |
| id | doaj.art-822c8c1565f943de91b19820ef0610f2 |
| institution | Directory Open Access Journal |
| issn | 2045-2322 |
| language | English |
| last_indexed | 2024-03-13T09:02:45Z |
| publishDate | 2023-05-01 |
| publisher | Nature Portfolio |
| record_format | Article |
| series | Scientific Reports |
| spelling | doaj.art-822c8c1565f943de91b19820ef0610f22023-05-28T11:12:45ZengNature PortfolioScientific Reports2045-23222023-05-0113111010.1038/s41598-023-35171-yEmergence of community-associated methicillin-resistant Staphylococcus aureus ΨUSA300 among Japanese people with HIV, resulted from stepwise mutations in 2010sKoh Shinohara0Yuki Uehara1Katsuji Teruya2Takashi Sasaki3Tadashi Baba4Hidemasa Nakaminami5Pegah Kananizadeh6Yuh Morimoto7Yoshimi Kikuchi8Shinichi Oka9AIDS Clinical Center, The National Center for Global Health and MedicineDepartment of Microbiology, Faculty of Medicine, Juntendo UniversityAIDS Clinical Center, The National Center for Global Health and MedicineAnimal Research Center, Sapporo Medical University School of MedicineGraduate School of Nursing, Seisen Jogakuin CollegeDepartment of Microbiology, School of Pharmacy, Tokyo University of Pharmacy and Life SciencesDepartment of Microbiology, Faculty of Medicine, Juntendo UniversityFaculty of Health Science, Juntendo UniversityAIDS Clinical Center, The National Center for Global Health and MedicineAIDS Clinical Center, The National Center for Global Health and MedicineAbstract Although infection with the methicillin-resistant Staphylococcus aureus (MRSA) clone USA300 is extremely rare in Japan, the uniquely evolved clone ΨUSA300 has been reported in Japan. An outbreak of a distinct USA300 clone was recently reported in an HIV/AIDS referral hospital in Tokyo. The present study investigated the evolutionary origin and genetic diversity of USA300-related clones causing regional outbreaks among people living with HIV (PLWHIV) in Tokyo. MRSA isolates collected from PLWHIV in an HIV/AIDS referral center in Tokyo were subjected to whole-genome sequencing and their genetic features were compared with those of previously described USA300 MRSA genomes. Of the 28 MRSAs isolated in 2016–2019, 23 (82.1%) were identified as USA300, with 22 (95.6%) of the latter identified as ΨUSA300. Although the genomic structure of ΨUSA300 was identical to the structures of reference USA300 strains, one clade (cluster A) was found to have acquired 29 previously identified lineage-specific mutations in a stepwise manner. The estimated divergence dates of ΨUSA300 and Cluster A were 2009 and 2012, respectively. These findings suggested that the ΨUSA300 clone had spread among PLWHIVs in Tokyo in the early 2010s, with stepwise acquisition of lineage-specific nonsynonymous mutations.https://doi.org/10.1038/s41598-023-35171-y |
| spellingShingle | Koh Shinohara Yuki Uehara Katsuji Teruya Takashi Sasaki Tadashi Baba Hidemasa Nakaminami Pegah Kananizadeh Yuh Morimoto Yoshimi Kikuchi Shinichi Oka Emergence of community-associated methicillin-resistant Staphylococcus aureus ΨUSA300 among Japanese people with HIV, resulted from stepwise mutations in 2010s Scientific Reports |
| title | Emergence of community-associated methicillin-resistant Staphylococcus aureus ΨUSA300 among Japanese people with HIV, resulted from stepwise mutations in 2010s |
| title_full | Emergence of community-associated methicillin-resistant Staphylococcus aureus ΨUSA300 among Japanese people with HIV, resulted from stepwise mutations in 2010s |
| title_fullStr | Emergence of community-associated methicillin-resistant Staphylococcus aureus ΨUSA300 among Japanese people with HIV, resulted from stepwise mutations in 2010s |
| title_full_unstemmed | Emergence of community-associated methicillin-resistant Staphylococcus aureus ΨUSA300 among Japanese people with HIV, resulted from stepwise mutations in 2010s |
| title_short | Emergence of community-associated methicillin-resistant Staphylococcus aureus ΨUSA300 among Japanese people with HIV, resulted from stepwise mutations in 2010s |
| title_sort | emergence of community associated methicillin resistant staphylococcus aureus ψusa300 among japanese people with hiv resulted from stepwise mutations in 2010s |
| url | https://doi.org/10.1038/s41598-023-35171-y |
| work_keys_str_mv | AT kohshinohara emergenceofcommunityassociatedmethicillinresistantstaphylococcusaureuspsusa300amongjapanesepeoplewithhivresultedfromstepwisemutationsin2010s AT yukiuehara emergenceofcommunityassociatedmethicillinresistantstaphylococcusaureuspsusa300amongjapanesepeoplewithhivresultedfromstepwisemutationsin2010s AT katsujiteruya emergenceofcommunityassociatedmethicillinresistantstaphylococcusaureuspsusa300amongjapanesepeoplewithhivresultedfromstepwisemutationsin2010s AT takashisasaki emergenceofcommunityassociatedmethicillinresistantstaphylococcusaureuspsusa300amongjapanesepeoplewithhivresultedfromstepwisemutationsin2010s AT tadashibaba emergenceofcommunityassociatedmethicillinresistantstaphylococcusaureuspsusa300amongjapanesepeoplewithhivresultedfromstepwisemutationsin2010s AT hidemasanakaminami emergenceofcommunityassociatedmethicillinresistantstaphylococcusaureuspsusa300amongjapanesepeoplewithhivresultedfromstepwisemutationsin2010s AT pegahkananizadeh emergenceofcommunityassociatedmethicillinresistantstaphylococcusaureuspsusa300amongjapanesepeoplewithhivresultedfromstepwisemutationsin2010s AT yuhmorimoto emergenceofcommunityassociatedmethicillinresistantstaphylococcusaureuspsusa300amongjapanesepeoplewithhivresultedfromstepwisemutationsin2010s AT yoshimikikuchi emergenceofcommunityassociatedmethicillinresistantstaphylococcusaureuspsusa300amongjapanesepeoplewithhivresultedfromstepwisemutationsin2010s AT shinichioka emergenceofcommunityassociatedmethicillinresistantstaphylococcusaureuspsusa300amongjapanesepeoplewithhivresultedfromstepwisemutationsin2010s |