Assessment and Imaging of Intracellular Magnesium in SaOS-2 Osteosarcoma Cells and Its Role in Proliferation
Magnesium is an essential nutrient involved in many important processes in living organisms, including protein synthesis, cellular energy production and storage, cell growth and nucleic acid synthesis. In this study, we analysed the effect of magnesium deficiency on the proliferation of SaOS-2 osteo...
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MDPI AG
2021-04-01
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| Series: | Nutrients |
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| Online Access: | https://www.mdpi.com/2072-6643/13/4/1376 |
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| author | Concettina Cappadone Emil Malucelli Maddalena Zini Giovanna Farruggia Giovanna Picone Alessandra Gianoncelli Andrea Notargiacomo Michela Fratini Carla Pignatti Stefano Iotti Claudio Stefanelli |
| author_facet | Concettina Cappadone Emil Malucelli Maddalena Zini Giovanna Farruggia Giovanna Picone Alessandra Gianoncelli Andrea Notargiacomo Michela Fratini Carla Pignatti Stefano Iotti Claudio Stefanelli |
| author_sort | Concettina Cappadone |
| collection | DOAJ |
| description | Magnesium is an essential nutrient involved in many important processes in living organisms, including protein synthesis, cellular energy production and storage, cell growth and nucleic acid synthesis. In this study, we analysed the effect of magnesium deficiency on the proliferation of SaOS-2 osteosarcoma cells. When quiescent magnesium-starved cells were induced to proliferate by serum addition, the magnesium content was 2–3 times lower in cells maintained in a medium without magnesium compared with cells growing in the presence of the ion. Magnesium depletion inhibited cell cycle progression and caused the inhibition of cell proliferation, which was associated with mTOR hypophosphorylation at Serine 2448. In order to map the intracellular magnesium distribution, an analytical approach using synchrotron-based X-ray techniques was applied. When cell growth was stimulated, magnesium was mainly localized near the plasma membrane in cells maintained in a medium without magnesium. In non-proliferating cells growing in the presence of the ion, high concentration areas inside the cell were observed. These results support the role of magnesium in the control of cell proliferation, suggesting that mTOR may represent an important target for the antiproliferative effect of magnesium. Selective control of magnesium availability could be a useful strategy for inhibiting osteosarcoma cell growth. |
| first_indexed | 2024-03-10T12:10:41Z |
| format | Article |
| id | doaj.art-822d211ae8e94a0e8ad5d7c4b3ebb760 |
| institution | Directory Open Access Journal |
| issn | 2072-6643 |
| language | English |
| last_indexed | 2024-03-10T12:10:41Z |
| publishDate | 2021-04-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Nutrients |
| spelling | doaj.art-822d211ae8e94a0e8ad5d7c4b3ebb7602023-11-21T16:15:55ZengMDPI AGNutrients2072-66432021-04-01134137610.3390/nu13041376Assessment and Imaging of Intracellular Magnesium in SaOS-2 Osteosarcoma Cells and Its Role in ProliferationConcettina Cappadone0Emil Malucelli1Maddalena Zini2Giovanna Farruggia3Giovanna Picone4Alessandra Gianoncelli5Andrea Notargiacomo6Michela Fratini7Carla Pignatti8Stefano Iotti9Claudio Stefanelli10Department of Pharmacy and Biotechnology, University of Bologna, 33, 40127 Bologna, ItalyDepartment of Pharmacy and Biotechnology, University of Bologna, 33, 40127 Bologna, ItalyDepartment of Biomedical and Neuromotor Sciences, Alma Mater Studiorum, University of Bologna, 33, 40126 Bologna, ItalyDepartment of Pharmacy and Biotechnology, University of Bologna, 33, 40127 Bologna, ItalyDepartment of Pharmacy and Biotechnology, University of Bologna, 33, 40127 Bologna, ItalyElettra—Sincrotrone Trieste, 34149 Trieste, ItalyInstitute for Photonics and Nanotechnologies, Consiglio Nazionale delle Ricerche, 00156 Rome, ItalyInstitute of Nanotechnology-CNR c/o Physics Department at ‘Sapienza’ University, 00185 Rome, ItalyDepartment of Biomedical and Neuromotor Sciences, Alma Mater Studiorum, University of Bologna, 33, 40126 Bologna, ItalyDepartment of Pharmacy and Biotechnology, University of Bologna, 33, 40127 Bologna, ItalyDepartment for Life Quality Studies, Alma Mater Studiorum, University of Bologna, 47921 Rimini, ItalyMagnesium is an essential nutrient involved in many important processes in living organisms, including protein synthesis, cellular energy production and storage, cell growth and nucleic acid synthesis. In this study, we analysed the effect of magnesium deficiency on the proliferation of SaOS-2 osteosarcoma cells. When quiescent magnesium-starved cells were induced to proliferate by serum addition, the magnesium content was 2–3 times lower in cells maintained in a medium without magnesium compared with cells growing in the presence of the ion. Magnesium depletion inhibited cell cycle progression and caused the inhibition of cell proliferation, which was associated with mTOR hypophosphorylation at Serine 2448. In order to map the intracellular magnesium distribution, an analytical approach using synchrotron-based X-ray techniques was applied. When cell growth was stimulated, magnesium was mainly localized near the plasma membrane in cells maintained in a medium without magnesium. In non-proliferating cells growing in the presence of the ion, high concentration areas inside the cell were observed. These results support the role of magnesium in the control of cell proliferation, suggesting that mTOR may represent an important target for the antiproliferative effect of magnesium. Selective control of magnesium availability could be a useful strategy for inhibiting osteosarcoma cell growth.https://www.mdpi.com/2072-6643/13/4/1376magnesiumosteosarcomacell cyclemTOR |
| spellingShingle | Concettina Cappadone Emil Malucelli Maddalena Zini Giovanna Farruggia Giovanna Picone Alessandra Gianoncelli Andrea Notargiacomo Michela Fratini Carla Pignatti Stefano Iotti Claudio Stefanelli Assessment and Imaging of Intracellular Magnesium in SaOS-2 Osteosarcoma Cells and Its Role in Proliferation Nutrients magnesium osteosarcoma cell cycle mTOR |
| title | Assessment and Imaging of Intracellular Magnesium in SaOS-2 Osteosarcoma Cells and Its Role in Proliferation |
| title_full | Assessment and Imaging of Intracellular Magnesium in SaOS-2 Osteosarcoma Cells and Its Role in Proliferation |
| title_fullStr | Assessment and Imaging of Intracellular Magnesium in SaOS-2 Osteosarcoma Cells and Its Role in Proliferation |
| title_full_unstemmed | Assessment and Imaging of Intracellular Magnesium in SaOS-2 Osteosarcoma Cells and Its Role in Proliferation |
| title_short | Assessment and Imaging of Intracellular Magnesium in SaOS-2 Osteosarcoma Cells and Its Role in Proliferation |
| title_sort | assessment and imaging of intracellular magnesium in saos 2 osteosarcoma cells and its role in proliferation |
| topic | magnesium osteosarcoma cell cycle mTOR |
| url | https://www.mdpi.com/2072-6643/13/4/1376 |
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